Abstract:
:Biologically active heart valves (tissue engineered and recellularized tissue-derived heart valves) have the potential to offer enhanced function when compared to current replacement value therapies since they can possibly remodel, and grow to meet the needs of the patient, and not require chronic medication. However, this technology is still in its infancy and many fundamental questions remain as to how these valves will function in vivo. It has been shown that exposing biologically active tissue constructs to pulsatile pressures and flows during in vitro culture produces enhanced extracellular matrix protein expression and cellularity, although the ideal hydrodynamic conditioning regime is as yet unknown. Moreover, in vitro organ-level studies of living heart valves aimed at studying the remodeling processes require environments that can accurately reproduce in vivo hemodynamics under sterile conditions. To this end, we have developed a system to study the effects of subjecting biologically active heart valves to highly controlled pulsatile pressure and flow waveforms under sterile conditions. The device fits inside a standard incubator and utilizes a computer-controlled closed loop feedback system to provide a high degree of control. The mean pressure, mean flow rate, driving frequency, and shape of the pulsatile pressure waveform can be changed automatically in order to simulate both physiologic and nonphysiologic hemodynamic conditions. Extensive testing and evaluation demonstrated the device's ability to subject a biologically active heart valve to highly controlled pulsatile waveforms that can be modulated during the course of sterile incubation.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Hildebrand DK,Wu ZJ,Mayer JE Jr,Sacks MSdoi
10.1114/b:abme.0000036640.11387.4bsubject
Has Abstractpub_date
2004-08-01 00:00:00pages
1039-49issue
8eissn
0090-6964issn
1573-9686journal_volume
32pub_type
杂志文章abstract::This paper presents an in vitro temperature mapping study of bovine cardiac tissue during radiofrequency ablation. The objectives were to: (i) develop a technique for measuring the spatial and temporal temperature distribution in the tissue and in the blood during ablation, and (ii) use the temperature measurements to...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1310218
更新日期:2000-09-01 00:00:00
abstract::It would often be desirable to obtain the respiratory rate during everyday conditions without obtaining an additional respiratory trace. This study investigates the agreement between respiratory rate assessed from the electrocardiogram (ECG) and the reference respiratory rate derived from a nasal/oral airflow (AF). Na...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-008-9580-2
更新日期:2008-12-01 00:00:00
abstract::Further understanding of the mechanical deformation and tear behavior of kidney during suturing helps to enhance surgical task execution that requires fine suture manipulation. This paper aims to develop a model to describe the relationship between the tensile force acting on suture line and the resulted kidney deform...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-012-0722-1
更新日期:2013-04-01 00:00:00
abstract::This study presents a new steady-state visual evoked potential (SSVEP)-based brain computer interface (BCI). SSVEPs, induced by phase-tagged flashes in eight light emitting diodes (LEDs), were used to control four cursor movements (up, right, down, and left) and four button functions (on, off, right-, and left-clicks)...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-9964-y
更新日期:2010-07-01 00:00:00
abstract::The responses of photoreceptor cells to moving stimuli are crucial to understanding motion detection in visual systems. However, these responses are not well characterized quantitatively because they result from a combination of spatial optical behavior in the lens systems with temporal behavior in the phototransducti...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.58
更新日期:1998-03-01 00:00:00
abstract::Induced pluripotent stem cell derived cardiomyocytes (iPSC-CM) provide a powerful platform for disease modeling and drug development in vitro. Traditionally, electrophysiological methods or fluorescent dyes (e.g. calcium) have been used in their functional characterization. Recently, video microscopy has enabled non-i...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-017-1933-2
更新日期:2018-01-01 00:00:00
abstract::In this paper, a novel cell stretcher design that mimics the real-time stretch of the heart wall is introduced. By culturing cells under stretched conditions that mimics the mechanical aspects of the native cardiac environment, better understanding on the role of biomechanical signaling on cell development can be achi...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-014-0976-x
更新日期:2014-05-01 00:00:00
abstract::To study the effects of compressive straining on muscle cell deformation and damage an in vitro model system was developed. Myoblasts were seeded in agarose constructs and cultured in growth medium for 4 days. Subsequently, the cells were allowed to fuse into multinucleated myotubes for 8 days in differentiation mediu...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1349698
更新日期:2001-02-01 00:00:00
abstract::Phenotype transformation of vascular smooth muscle cells (VSMCs) has been reported to be directly influenced by the frequency of mechanical strain. This study explored the effects of different frequencies of mechanical strain on expression of phenotype marker h1-calponin and the possible mechanism. VSMCs were subjecte...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-008-9521-0
更新日期:2008-09-01 00:00:00
abstract::Abnormal scapular movement is widely believed to be an important factor in clinical pathology of the shoulder joint complex. Validated non-invasive techniques for measuring scapular movement have been developed, but the effect of marker placement on accuracy is unknown. The objective of this study was to determine the...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-0185-1
更新日期:2011-02-01 00:00:00
abstract::This paper presents the permeability analysis of neuroactive drugs and correlation with in vivo brain/plasma ratios in a dynamic microfluidic blood-brain barrier (BBB) model. Permeability of seven neuroactive drugs (Ethosuximide, Gabapentin, Sertraline, Sunitinib, Traxoprodil, Varenicline, PF-304014) and trans-endothe...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-014-1086-5
更新日期:2014-12-01 00:00:00
abstract::Experimental and diagnostic procedures like distension of a balloon catheter, bougie, and esophagogastroduodenoscopy can induce shear deformation in the esophageal wall. However, the shear modulus of the esophagus is yet unknown. The aim of this study was to determine the esophageal shear modulus and its dependence on...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/b:abme.0000039356.24821.6c
更新日期:2004-09-01 00:00:00
abstract::Baseball players swung very light and very heavy bats through our instrument and the speed of the bat was recorded. These data were used to make mathematical models for each person. Then these models were coupled with equations of physics for bat-ball collisions to compute the Ideal Bat Weight for each individual. How...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584443
更新日期:1995-07-01 00:00:00
abstract::To better understand the relationship between kinetic processes of contraction and the dynamic features of an isometric twitch, studies were conducted using a mathematical model that included: (1) kinetics of cross bridge (XB) cycling; (2) kinetics of thin filament regulatory processes; (3) serial and feedback interac...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1366669
更新日期:2001-05-01 00:00:00
abstract::We compare the influence of time-frequency methods on analysis of time-varying renal autoregulation properties. Particularly, we examine if detection probabilities are similar for amplitude and frequency modulation for a modulated simulation signal among five time-frequency approaches, and if time-varying changes in s...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-012-0625-1
更新日期:2013-01-01 00:00:00
abstract::Previous research in cellular adhesion has focused primarily on studying isolated cells under conditions where cells do not interact with each other. However, in vivo cells form sheets where both cell-substratum and cell-cell interactions contribute to the overall adhesive behavior. Our understanding of how cell-cell ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.178
更新日期:1999-03-01 00:00:00
abstract::Measurement of cell shortening is an important technique for assessment of physiology and pathophysiology of cardiac myocytes. Many types of heart disease are associated with decreased myocyte shortening, which is commonly caused by structural and functional remodeling. Here, we present a new approach for local measur...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-016-1593-7
更新日期:2016-10-01 00:00:00
abstract::Recent studies suggest that vortex ring formation during left ventricular (LV) rapid filling is an optimized mechanism for blood transport, and that the volume of the vortex ring is an important measure. However, due to lack of quantitative methods, the volume of the vortex ring has not previously been studied. Lagran...
journal_title:Annals of biomedical engineering
pub_type: 临床试验,杂志文章
doi:10.1007/s10439-012-0615-3
更新日期:2012-12-01 00:00:00
abstract::Linear compartmental models are useful, explanatory tools, that have been widely used to represent the dynamic behavior of complex biological systems. This paper addresses the problem of the numerical identification of such models, i.e., the estimation of the parameter values that will generate predictions closest to ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-009-9651-z
更新日期:2009-05-01 00:00:00
abstract::Magnetic nanoparticle (mNP) based thermal therapies have demonstrated relevance in the clinic, but effective application requires an understanding of both its strengths and limitations. This study explores two critical limitations for clinical use: (1) maximizing localized mNP heating, while avoiding bulk heating due ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-012-0633-1
更新日期:2013-01-01 00:00:00
abstract::Parameters of physiological models are commonly associated in an input-output experiment with a specific pattern of the system response. This association is often made on an intuitive basis by traditional sensitivity analysis, i.e., by inspecting the variations of model output trajectories with respect to parameter va...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.207
更新日期:1999-09-01 00:00:00
abstract::The objectives were as follows: (1) estimating mean value of the overall hemoglobin glycation rate constant (k); (2) analyzing inter-individual variability of k; (3) verifying ability of the hemoglobin A1c (HbA1c) formation model to predict changes of HbA1c during red blood cells cultivation in vitro and to reproduce ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0366-6
更新日期:2011-11-01 00:00:00
abstract::Rapid technological advances have prompted the development of a wide range of telemonitoring systems to enable the prevention, early diagnosis and management, of chronic conditions. Remote monitoring can reduce the amount of recurring admissions to hospital, facilitate more efficient clinical visits with objective res...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
doi:10.1007/s10439-005-9068-2
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:We present a fundamental theoretical framework for analysis of energy dissipation in any component of the circulatory system and formulate the full energy budget for both venous and arterial circulations. New indices allowing disease-specific subject-to-subject comparisons and disease-to-disease hemodynamic ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-009-9650-0
更新日期:2009-04-01 00:00:00
abstract::The bioinspired, microscale tissue engineering approach has emerged in recent years as a promising alternative to preformed scaffolds. Using this approach, complex tissues and organs can be efficiently engineered from microscale modules to replicate the intricate architecture and physiology of vascularized organs and ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-018-02192-y
更新日期:2019-05-01 00:00:00
abstract::Polycrystalline silicon (poly-Si) micromechanical structures can be made by selectively etching an underlying sacrificial oxide layer. Advantages of this micromachining technique are its simplicity and compatibility with conventional integrated-circuit processing. Compressive internal stress in poly-Si films constrain...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584269
更新日期:1986-01-01 00:00:00
abstract::Phospholipids have been identified by thin-layer chromatography in appreciable quantities in pericardial fluid taken from 12 dogs and found to include sphingomyelin and phosphatidylcholines, -ethanolamines, -inositols and -serines--the cholines predominating. The extracts, the synthetic surfactants and a mixture of sy...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584258
更新日期:1985-01-01 00:00:00
abstract::The pia-arachnoid complex (PAC) covering the brain plays an important role in the mechanical response of the brain during impact or inertial loading. Recent studies have revealed the complicated material behavior of the PAC. In this study, the nonlinear viscoelastic, transversely isotropic material properties of the P...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-013-0948-6
更新日期:2014-04-01 00:00:00
abstract::It is well known that neutrophils take much longer to traverse the pulmonary capillary bed than erythrocytes, and this is likely due to differences in the structure and rheology of the cells. In this study, we simulate the transit of a neutrophil in a capillary using a Newtonian drop model and a viscoelastic drop mode...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-007-9286-x
更新日期:2007-05-01 00:00:00
abstract::Conventional nanoporous hydrogels often lead to slow cartilage deposition by MSCs in 3D due to physical constraints and requirement for degradation. Our group has recently reported macroporous gelatin microribbon (μRB) hydrogels, which substantially accelerate MSC-based cartilage formation in vitro compared to convent...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-020-02522-z
更新日期:2020-07-01 00:00:00