Abstract:
:Purpose: The purpose of this study is to explore the effects of dihydrotestosterone (DHT) on lipopolysaccharide (LPS)-induced proinflammatory cytokine release in human ocular surface epithelial cells exposed to LPS and LPS-binding protein (LBP).Methods: Immortalized human corneal, conjunctival, and meibomian gland epithelial cells were cultured in keratinocyte-free medium. After confluency, they were exposed to a stratification medium Dulbecco's modified Eagle medium (DMEM)/F12 in the presence of fetal bovine serum and were exposed to vehicle, LPS + LBP, or DHT. Culture media were processed for multiplex-bead analysis of specific proinflammatory cytokines including interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, IL-4, IL-8, IL-6, IL-10, IL-1β, vascular endothelial growth factor (VEGF)-A. Cytokine concentrations were compared by analysis of variance with Tukey post hoc testing. p < 0.05 was considered statistically significant.Results: The results are LPS + LBP-induced the secretion of IFN-γ, IL-6, IL-10, IL-1β, VEGF-A cytokines in corneal epithelial cells; TNF-α, IL-2, IL-8, IL-6, IL-1β, VEGF-A cytokines in conjunctival epithelial cells; and IL-8, IL-6, IL-1β, VEGF-A cytokines in meibomian gland epithelial cells. When these LPS + LBP-stimulated cells were exposed to DHT for 2 days, it was found that DHT suppressed the secretion of IL-6, IL-10, IL-1β, VEGF-A cytokines in corneal epithelial cells; TNF-α, IL-6, IL-1β, VEGF-A cytokines in conjunctival epithelial cells; and IL-6, IL-1β, VEGF-A cytokines in meibomian gland epithelial cells.Conclusion: LPS + LBP is shown to induce the secretion of certain proinflammatory cytokines from ocular surface and adnexal epithelial cells. DHT showed anti-inflammatory activity by suppressing some of those cytokines in these cell lines.
journal_name
Ocul Immunol Inflammjournal_title
Ocular immunology and inflammationauthors
Marangoz D,Oner C,Schicht M,Turgut Cosan D,Paulsen F,Yildiz E,Zibandeh N,Sahin Adoi
10.1080/09273948.2019.1686155subject
Has Abstractpub_date
2019-11-18 00:00:00pages
1-9eissn
0927-3948issn
1744-5078pub_type
杂志文章abstract:PURPOSE:To report the clinical profile and management of patients diagnosed to have ampiginous choroiditis in a tertiary care referral centre in India. METHODS:Retrospective cohort study. Twenty-six eyes of 16 patients were included in the study, which was diagnosed as choroiditis, serpiginous choroiditis, and acute p...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273940903402637
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To evaluate the patterns of acute and recurrent acute anterior uveitis (AAU) in a metropolitan tertiary referral center. METHODS:A retrospective medical record review of a total of 241 patients with AAU presenting to Sydney Eye Hospital between June 2009 and June 2011 was performed. Patients who underwent typi...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2012.745882
更新日期:2013-04-01 00:00:00
abstract::Background Uveal effusion is a rare disease that is characterized by exudative detachment of the ciliary body and choroid. Herein, we report a rare case of uveal effusion associated with viral encephalitis, which resolved following the treatment of the viral encephalitis and administration of corticosteroids. Case pre...
journal_title:Ocular immunology and inflammation
pub_type: 信件
doi:10.1080/09273948.2020.1797110
更新日期:2020-08-20 00:00:00
abstract:BACKGROUND:To examine the effectiveness of prolonged oral acyclovir treatment in patients with acute retinal necrosis (ARN). METHODS:Fifty-five ARN patients (62 eyes) who had been treated with short-term or long-term oral acyclovir were identified in this retrospective study: one group treated with prolonged oral acyc...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2012.689073
更新日期:2012-08-01 00:00:00
abstract::The authors present a polymerase chain reaction method for rapid and direct diagnosis of herpetic intraocular infections using small volume samples of intraocular fluid from 29 patients with various intraocular inflammatory diseases and 24 controls with senile cataract. Of six patients with early acute retinal necrosi...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273949309086545
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:To investigate demographic, clinical features and complications of Italian patients with ocular Behçet's disease (BD) over time. METHODS:Retrospective study of 385 patients examined from 1968 to 2011, 265 of whom had follow-up ≥ 12 months. RESULTS:A significant increase in the proportion of females (p = 0.03)...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2015.1094095
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:To evaluate the 10-year drug retention rate of infliximab (IFX) in Behçet's disease (BD)-related uveitis, the effect of a concomitant use of disease modifying anti-rheumatic drugs (DMARDs) on drug survival and differences according to the lines of biologic treatment. METHODS:Cumulative survival rates were stud...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2017.1391297
更新日期:2019-01-01 00:00:00
abstract::Onchocercal keratitis (river blindness) is one of the leading worldwide causes of blindness. Light microscopic analysis of human specimens and corneal tissue from experimental models has implicated the eosinophil as an important cell in the inflammatory response. Our previous studies in experimental murine onchocercal...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273949709116890
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:To evaluate the effects of fluorescein fundus angiography (FFA) on semiautomated aqueous flare measurements. METHODS:Laser flare photometer (LFP) measurements was performed at baseline, 30 min, and 4 h after the intravenous administration of sodium fluorescein dye. FFA was performed immediately after the basel...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2020.1799036
更新日期:2020-09-23 00:00:00
abstract:PURPOSE:ICON is a fusion protein composed of factor VII, the natural ligand for tissue factor, conjugated to the Fc domain of a human IgG1 immunoglobulin. It binds to the tissue factor expressed on neovascular endothelia and initiates a cytolytic immune attack that destroys the neovascular tissue. We previously showed ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273940601147760
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:To report a case of subretinal fibrosis and uveitis syndrome in association with ectodermal dysplasia. METHODS:Chart review of a patient with subretinal fibrosis and uveitis syndrome and ectodermal dysplasia. RESULTS:The authors identified one case of a patient with the diagnoses of subretinal fibrosis and uv...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273940903168670
更新日期:2009-09-01 00:00:00
abstract:PURPOSE:Investigate the efficacy of intravitreal adalimumab in breakthrough panuveitis in patients on systemic adalimumab for more than 3 months. METHODS:Retrospective study of patients on systemic adalimumab with breakthrough panuveitis requiring intravitreal adalimumab therapy. RESULTS:Seven eyes of four patients w...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2017.1335756
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:To analyze the diagnostic predictive ability of clinical variables. METHODS:Demographic and clinical variables of 172 serologically proven leptospiral uveitis patients were compared with 200 controls of non-leptospiral uveitis. Multiple logistic regression analysis identified diagnostic predictors. A receiver ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2016.1217341
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:To characterize nodular lesions of the retina and optic nerve with spectral-domain optical coherence tomography (SD-OCT) in patients with sarcoidosis. METHODS:This is a retrospective series of 6 eyes from 5 patients with an established diagnosis of sarcoidosis, with clinically detected nodules of the optic ner...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2014.971972
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:An HLA-DQ1 association has been suspected in patients who develop Propionibacterium acnes endophthalmitis following cataract surgery. New techniques exist which may help to define this association. MATERIALS AND METHODS:Patients with culture-proven P. acnes endophthalmitis following cataract surgery were HL...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273949609069124
更新日期:1996-01-01 00:00:00
abstract::We determined the referral pattern and visual prognosis for patients with uveitis at a tertiary university eye center in the Midwestern U.S. over a five-year period. A retrospective chart review was conducted on 853 patients seen in the Department of Ophthalmology and Visual Sciences (DOVS) at Washington University an...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1076/ocii.11.4.287.18270
更新日期:2003-12-01 00:00:00
abstract::Aim: Ocular sarcoidosis presents a diagnostic challenge because of its varied clinical presentations. It is important to distinguish sarcoidosis from other uveitis diseases. Multimodal imaging provides useful data to be introduced into clinical practice. Methods: This is a review article. Conclusions: This review arti...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2020.1751210
更新日期:2020-11-16 00:00:00
abstract::Purpose: The role of HIV infection in exogenous and endogenous endophthalmitis has not been clarified. We aim to assess the potential role of HIV as a risk factor or a poor prognostic feature in this sight-threatening condition. Methods: Literature review. Review of evidence: Evidence for endophthalmitis in HIV patien...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2019.1699580
更新日期:2020-10-02 00:00:00
abstract:PURPOSE:Ipilimumab, a monoclonal antibody directed against the immune protein cytotoxic T-lymphocyte antigen-4 (CTLA-4), characteristically induces side effects called "immune-related adverse events" (IRAE). Although ophthalmic involvement is rare, we report 7 cases of eye and orbit complications related to ipilimumab ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章,评审
doi:10.3109/09273948.2014.1001858
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:To present a population of patients sharing atypical manifestations of vitreoretinal lymphoma (VRL). METHODS:Institutional case series in a single tertiary center. Patients with cytologically proven VRL, referred between November 2009 and May 2010, were retrospectively reviewed. Diagnosis of VRL was based on c...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2013.835427
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:To evaluate the safety and efficacy of intravitreal triamcinolone acetonide (TA) for treating macular edema secondary to non-infectious uveitis. METHODS:Retrospective review of sixteen patients (20 eyes) with chronic cystoid macular edema (CME) as a consequence of controlled intermediate uveitis, posterior ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273940590933511
更新日期:2005-04-01 00:00:00
abstract::Purpose: To emphasize the different manifestations of ocular involvement in Whipple disease with challenge in establishing the diagnosis as clinical, laboratory, and histological features could mimic other uveitis entities. Methods: Case reports of three patients. Results: The first patient was an African male sufferi...
journal_title:Ocular immunology and inflammation
pub_type: 信件
doi:10.1080/09273948.2018.1518461
更新日期:2019-01-01 00:00:00
abstract::Purpose: The purpose of the study is to quantify aqueous flare using laser flare photometry (LFM) in patients undergoing 25-G pars plana vitrectomy (PPV) and assess the need for postoperative topical corticosteroid administration . Methods: Prospective evaluation of 50 eyes (50 patients) was performed using LFM until ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2018.1449867
更新日期:2019-01-01 00:00:00
abstract::Purpose: To determine the anti-inflammatory effects of dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor-κB (NF-κB) inhibitor, on endotoxin-induced uveitis (EIU) in rats.Methods: EIU was induced by a subcutaneous injection of lipopolysaccharide (LPS) in Lewis rats. DHMEQ was injected intraperitoneally concurren...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2019.1568502
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:To investigate serum inflammatory factors in patients with idiopathic choroidal vascularization (CNV), including vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα), Interleukin1-beta (IL1-β), IgG, IgA, IgM, IgE, 50% hemolytic unit of complement (CH50), complement C3, complement C4 a...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273948.2010.483315
更新日期:2010-10-01 00:00:00
abstract:PURPOSE:To investigate the safety and efficacy of daclizumab (Zenapax, humanized anti-Tac, HAT) in controlling the ocular manifestations of Behçet's disease. DESIGN:Randomized, placebo-controlled, double-masked clinical trial. PARTICIPANTS:Seventeen participants with Behçet's disease experiencing at least two prior o...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章,随机对照试验
doi:10.1080/09273940701299370
更新日期:2007-03-01 00:00:00
abstract::Purpose: To evaluate retinal and choroidal microvascular features of VKH patients in acute and convalescent phases after treatment using OCTA.Methods: A prospective, observational study was conducted in patients with initial VKH at the acute stage (n = 15) and healthy participants (n = 15) served as controls. After 3-...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2019.1695856
更新日期:2020-01-03 00:00:00
abstract::Purpose: To describe tuberculous uveitis (TU) presenting as a bullous retinal detachment (RD) and to perform a comprehensive literature review on TU with similar features. Methods: Observational case report and systematic literature review. Results: An 84-year-old woman presented with bilateral granulomatous uveitis a...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.1080/09273948.2018.1485958
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:To describe four cases of familial Juvenile Idiopathic Arthritis (JIA) associated uveitis. DESIGN:Retrospective observational case series. METHODS:Clinical data from patients who present familial JIA-associated uveitis are described and possible genetic influence in disease etiology is reviewed. RESULTS:Two ...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273941003678837
更新日期:2010-06-01 00:00:00
abstract::Behçet's disease is the most frequently encountered form of endogenous uveitis in Japan. The authors examined the clinical manifestations in cases with Behçet's disease over six years (1987-1992), and compared the results with those of a previous study (1978-1983). In the more recent study the number of new patients w...
journal_title:Ocular immunology and inflammation
pub_type: 杂志文章
doi:10.3109/09273949509069117
更新日期:1995-01-01 00:00:00