Mechanistic details of amino acid catalyzed two-component Mannich reaction: experimental study backed by density functional calculations.

abstract：:The unusual basic amino acid, hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)-lysine], is a modified lysine with the addition of the 4-aminobutyl moiety from the polyamine spermidine. This naturally occurring amino acid is a product of a unique posttranslational modification that occurs in only one cellular protein, euk...

journal_title：Amino acids

authors： Park MH,Nishimura K,Zanelli CF,Valentini SR

更新日期：2010-02-01 00:00:00

abstract：:The relationship between taurine concentrations of plasma, whole blood, serum and skeletal muscle during taurine depletion and repletion was investigated in cats, to identify the most useful indicators of taurine status. Sixteen cats were fed a purified diet containing either 0 or 0.15 g/kg taurine for 5 months. Treat...

journal_title：Amino acids

authors： Pacioretty L,Hickman MA,Morris JG,Rogers QR

更新日期：2001-12-01 00:00:00

abstract：:Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. 3.1.2.26, protein BlsacA), which has no cr...

journal_title：Amino acids

authors： Mera A,de Lima MZT,Bernardes A,Garcia W,Muniz JRC

更新日期：2019-04-01 00:00:00

abstract：:The expression of wild type CFTR facilitates the efflux of neutral amino acids (Rotoli et al., Biochem. Biophys. Res. Commun. 204: 653-658, 1994); as a result, after an extensive depletion of intracellular amino acid pool obtained through an incubation in saline solution, the intracellular leucine levels were lower in...

journal_title：Amino acids

authors： Rotoli BM,Bussolati O,Cabrini G,Gazzola GC

更新日期：1996-06-01 00:00:00

abstract：:Reaction between glutathione and ribose was carried out to study the volatiles formed via Maillard reaction and their antioxidant activity as well as their role in inhibition of LDL oxidation. The simultaneous distillation-extraction technique was used for trapping the volatile components followed by GC-MS analysis. T...

journal_title：Amino acids

authors： El-massry K,Farouk A,El-Ghorab A

更新日期：2003-01-01 00:00:00

abstract：:D-Asp-containing proteins have been implicated in many aging-related diseases. To clarify the role of D-Asp-containing proteins in such diseases, we developed a screening system for these proteins using a D-aspartyl endopeptidase that specifically cleaves the proteins at the C-terminus. The digested proteins were dete...

journal_title：Amino acids

authors： Sakai-Kato K,Kinouchi T,Fujii N,Imai K,Utsunomiya-Tate N

更新日期：2009-01-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) are cell surface receptors. The dynamic property of receptor-receptor interactions in GPCRs modulates the kinetics of G-protein signaling and stability. In the present work, the structural and dynamic study of A(2A)R-D(2)R interactions was carried to acquire the understanding of the...

journal_title：Amino acids

authors： Prakash A,Luthra PM

更新日期：2012-10-01 00:00:00

abstract：:The aim of our research was to analyze the antioxidant role and efficacy of thermal or salus per aquam (spa) therapy with chlorine-sulphur-bicarbonate mineral water. The study has been performed on 30 rats. The animals were randomized in three groups, each of them composed by ten animals, denominated A, B and C. The A...

journal_title：Amino acids

authors： Costantino M,Giuberti G,Caraglia M,Lombardi A,Misso G,Abbruzzese A,Ciani F,Lampa E

更新日期：2009-02-01 00:00:00

abstract：:Hyperargininemia is caused by deficiency of arginase 1, which catalyzes the hydrolysis of L-arginine to urea as the final enzyme in the urea cycle. In contrast to other urea cycle defects, arginase 1 deficiency usually does not cause catastrophic neonatal hyperammonemia but rather presents with progressive neurologica...

journal_title：Amino acids

authors： Schlune A,Vom Dahl S,Häussinger D,Ensenauer R,Mayatepek E

更新日期：2015-09-01 00:00:00

abstract：:The enzymes Akt, mTOR, p70(S6K), rpS6, GSK3, and glycogen synthase interact in the control of protein and/or glycogen synthesis in skeletal muscle, and each has been found to respond to exercise and nutrient supplementation. In the present study, we tested the hypothesis that nutrient supplementation post exercise, in...

journal_title：Amino acids

authors： Ivy JL,Ding Z,Hwang H,Cialdella-Kam LC,Morrison PJ

更新日期：2008-06-01 00:00:00

abstract：:Antimicrobial peptides (AMPs) constitute a diverse class of naturally occurring or synthetic antimicrobial molecules that have potential for use in the treatment of drug-resistant infections. Several undesirable properties of AMPs, however, may ultimately hinder their development as antimicrobial agents. Thus, new syn...

journal_title：Amino acids

authors： Dong N,Zhu X,Lv YF,Ma QQ,Jiang JG,Shan AS

更新日期：2014-09-01 00:00:00

abstract：:Polyamines are essential for proliferation of endothelial cells (EC) and angiogenesis. This study was conducted to identify the metabolic source(s) of ornithine for polyamine synthesis in EC, using N(ω)-hydroxy-nor-L-arginine (Nor-NOHA, an inhibitor of arginase) and gabaculine (an inhibitor of ornithine aminotransfera...

journal_title：Amino acids

authors： Li H,Meininger CJ,Bazer FW,Wu G

更新日期：2016-10-01 00:00:00

abstract：:The putative translation factor eIF5A is essential for cell viability and is highly conserved from archaebacteria to mammals. This factor is the only cellular protein that undergoes an essential posttranslational modification dependent on the polyamine spermidine, called hypusination. This review focuses on the functi...

journal_title：Amino acids

authors： Zanelli CF,Valentini SR

更新日期：2007-08-01 00:00:00

abstract：:Carnosine is found in high concentrations in skeletal muscles, where it is involved in several physiological functions. The muscle carnosine content measured within a population can vary by a factor 4. The aim of this study was to further characterize suggested determinants of the muscle carnosine content (diet, gende...

journal_title：Amino acids

authors： Everaert I,Mooyaart A,Baguet A,Zutinic A,Baelde H,Achten E,Taes Y,De Heer E,Derave W

更新日期：2011-04-01 00:00:00

abstract：:Nitric oxide (NO) is a free radical gas synthesised from arginine and oxygen by enzymes of the family of the nitric oxide synthase. In particular, the neuronal nitric oxide synthase (nNOS) is highly expressed by cells of the hypothalamic paraventricular nucleus, where the sympatho-adrenal system, the hypothalamic-pitu...

journal_title：Amino acids

authors： Orlando GF,Wolf G,Engelmann M

更新日期：2008-06-01 00:00:00

abstract：:Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria....

journal_title：Amino acids

authors： Saavedra-Molina A,Ramírez-Emiliano J,Clemente-Guerrero M,Pérez-Vázquez V,Aguilera-Aguirre L,González-Hernández JC

更新日期：2003-01-01 00:00:00

abstract：:One pathogenic mechanism of ethanol-induced liver injury is the excessive production of reactive oxygen species (ROS), which may result in alcoholic liver disease (ALD) characterized by cell death due to necrosis and apoptosis. Taurine was proved to protect against liver damage. However, whether taurine attenuates eth...

journal_title：Amino acids

authors： Wu G,Yang J,Lv H,Jing W,Zhou J,Feng Y,Lin S,Yang Q,Hu J

更新日期：2018-07-01 00:00:00

abstract：:Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met i...

journal_title：Amino acids

authors： Kusano M,Fukushima A,Redestig H,Kobayashi M,Otsuki H,Onouchi H,Naito S,Hirai MY,Saito K

更新日期：2010-10-01 00:00:00

abstract：:This study tested the hypothesis that L-arginine (Arg) may stimulate cell proliferation and prevent lipopolysaccharide (LPS)-induced death of intestinal cells. Intestinal porcine epithelial cells (IPEC-1) were cultured for 4 days in Arg-free Dulbecco's modified Eagle's-F12 Ham medium (DMEM-F12) containing 10, 100 or 3...

journal_title：Amino acids

authors： Tan B,Yin Y,Kong X,Li P,Li X,Gao H,Li X,Huang R,Wu G

更新日期：2010-04-01 00:00:00

abstract：:Spore coat-associated alanine racemase of Bacillus subtilis, which converts L-alanine to D-alanine, that is, the germinant to the competitive inhibitor, to regulate spore germination for survival of the organism under unfavorable growth conditions, was examined. The dormant spores, L-alanine-initiated germination of w...

journal_title：Amino acids

authors： Kanda-Nambu K,Yasuda Y,Tochikubo K

更新日期：2000-01-01 00:00:00

abstract：:Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time exposure of UV irradiation decreases procollagen mRNA expression in dermis and that chronic UV irradiation decreases collagen amounts and induces wrinkle formation. ...

journal_title：Amino acids

authors： Murakami H,Shimbo K,Inoue Y,Takino Y,Kobayashi H

更新日期：2012-06-01 00:00:00

abstract：:On a biochemical level, conventional antidepressants have been shown to modulate synaptic levels of biogenic amines (i.e., serotonin, norepinephrine, and dopamine), most often by interfering with reuptake processes or inhibiting metabolism. Strategies directed at modulating glutamatergic transmission may overcome the ...

journal_title：Amino acids

authors： Skolnick P

更新日期：2002-01-01 00:00:00

abstract：:Breast cancer epidemic in the early twenty-first century results in around two million new cases and half-a-million of the disease-related deaths registered annually worldwide. A particularly dramatic situation is attributed to some specific patient subgroups such as the triple-negative breast cancer (TNBC). TNBC is a...

journal_title：Amino acids

authors： Golubnitschaja O,Filep N,Yeghiazaryan K,Blom HJ,Hofmann-Apitius M,Kuhn W

更新日期：2018-04-01 00:00:00

abstract：:Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...

journal_title：Amino acids

authors： Chang ML,Cheng ML,Chang SW,Tang HY,Chiu CT,Yeh CT,Shiao MS

更新日期：2017-02-01 00:00:00

abstract：:Creatine is an amino acid that has a pivotal role in energy metabolism of cells. Creatine acts as an "ATP shuttle", carrying ATP to the sites where it is utilized, through its reversible phosphorylation by creatine kinase. Moreover, the creatine-phosphocreatine system delays ATP depletion during anoxia or ischemia, th...

journal_title：Amino acids

authors： Garbati P,Salis A,Adriano E,Galatini A,Damonte G,Balestrino M,Millo E

更新日期：2013-10-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) plays a vital role in the progress of malignant disease and elevated Hsp90 expression has been reported in pancreatic cancer. In this study, we radiolabeled a dimeric Sansalvamide A derivative (Di-San A1) with 64Cu, and evaluated the feasibility of using 64Cu-Di-San A1 for PET imaging of ...

journal_title：Amino acids

authors： Wang X,Zhang J,Wu H,Li Y,Conti PS,Chen K

更新日期：2018-07-01 00:00:00

abstract：:Dopa and related catecholamines and their degradation products have been demonstrated to have neurotoxic potential in a number of cellular and in vivo experiments. Several mechanisms have been hypothesized to be involved including generation of prooxidant products that subsequently oxidize membrane lipids and exposed ...

journal_title：Amino acids

authors： VonVoigtlander PF,Fici GJ,Althaus JS

更新日期：1998-01-01 00:00:00

abstract：:The high efficiency of protein deposition during the neonatal period is driven by high rates of protein synthesis, which are maximally stimulated after feeding. In the current study, we examined the individual roles of amino acids and insulin in the regulation of protein synthesis in peripheral and visceral tissues of...

journal_title：Amino acids

authors： Suryawan A,O'Connor PM,Bush JA,Nguyen HV,Davis TA

更新日期：2009-05-01 00:00:00

abstract：:In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the...

journal_title：Amino acids

authors： Millioni R,Puricelli L,Iori E,Tessari P

更新日期：2008-04-01 00:00:00

abstract：:We examined the effects of DON [glutamine-analogue and inhibitor of glutamine-requiring enzymes], alanyl-glutamine (regarding its role in neutrophil immunonutrition) and alanyl-glutamine combined with L-NAME, SNAP, DON, beta-alanine and DFMO on neutrophil amino and alpha-keto acid concentrations or important neutrophi...

journal_title：Amino acids

authors： Mühling J,Burchert D,Langefeld TW,Matejec R,Harbach H,Engel J,Wolff M,Welters ID,Fuchs M,Menges T,Krüll M,Hempelmann G

更新日期：2007-09-01 00:00:00