Abstract:
:D-Asp-containing proteins have been implicated in many aging-related diseases. To clarify the role of D-Asp-containing proteins in such diseases, we developed a screening system for these proteins using a D-aspartyl endopeptidase that specifically cleaves the proteins at the C-terminus. The digested proteins were detected by means of two-dimensional gel electrophoresis and identified using nano-liquid chromatography/tandem mass spectrometry. We were able to detect myelin basic protein, a known D-Asp-containing protein, in the brain tissues of mice; this indicates that our system is effective for screening D-Asp-containing proteins.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Sakai-Kato K,Kinouchi T,Fujii N,Imai K,Utsunomiya-Tate Ndoi
10.1007/s00726-008-0040-ysubject
Has Abstractpub_date
2009-01-01 00:00:00pages
125-9issue
1eissn
0939-4451issn
1438-2199journal_volume
36pub_type
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