Enzyme promiscuity in earthworm serine protease: substrate versatility and therapeutic potential.

abstract：:Cell-penetrating peptides, a group of short peptides, can traverse cell membranes to enter cells and thus facilitate the uptake of various molecular cargoes. Thus, they have the potential to become powerful drug delivery systems. The correct identification of peptides as cell-penetrating or non-cell-penetrating would ...

journal_title：Amino acids

authors： Chen L,Chu C,Huang T,Kong X,Cai YD

更新日期：2015-07-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) are cell surface receptors. The dynamic property of receptor-receptor interactions in GPCRs modulates the kinetics of G-protein signaling and stability. In the present work, the structural and dynamic study of A(2A)R-D(2)R interactions was carried to acquire the understanding of the...

journal_title：Amino acids

authors： Prakash A,Luthra PM

更新日期：2012-10-01 00:00:00

abstract：:The presence of an enzyme activity which hydrolyzes glycyl-D-aspartate was found in the homogenates of pig kidney cortex. The activity was inhibited by metal chelating agents and cilastatin, suggesting that the enzyme was a cilastatin-sensitive metallo-peptidase. Of the two hydrolysis products,D-aspartate was found to...

journal_title：Amino acids

authors： Kera Y,Funabashi K,Matsumoto T,Watanabe T,Nagasaki H,Yamada R

更新日期：1996-06-01 00:00:00

abstract：:The present research has been carried out to elicit the mechanism of antiangiogenic activity of a fusion peptide P2. Peptide P2 was designed by the connection of a heptapeptide MMP inhibitor to ES-2, a fragment of Endostatin. In a previous study, P2 demonstrated strong antiangiogenic and antitumor effect, and the curr...

journal_title：Amino acids

authors： Xu Y,Qiang X,Xing L,Wang H,Zhang J,Zhang F,Caliskan B,Wang M,Qiu Z

更新日期：2018-08-01 00:00:00

abstract：:This work deals with the Dakin-West synthesis, starting from the nucleoamino acid 1-thyminyl acetic acid, as well the NMR, ESI MS, and X-ray characterization of a heteroaromatic compound denominated by us T(2)CO, comprising two thymine moieties anchored to a 2-propanonic unit, the spectroscopic properties of which wer...

journal_title：Amino acids

authors： Roviello GN,Roviello G,Musumeci D,Bucci EM,Pedone C

更新日期：2012-10-01 00:00:00

abstract：:Glutamine is the most abundant amino acid in the body. It is an important fuel for some key cells of the immune system. Both the plasma concentration of glutamine and the functional ability of immune cells in the blood are decreased after prolonged, exhaustive exercise. Glutamine feeding has had beneficial effects in ...

journal_title：Amino acids

authors： Castell LM,Newsholme EA

更新日期：2001-01-01 00:00:00

abstract：:Molecular imaging of human epidermal growth factor receptor type 2 (HER2) expression has drawn significant attention because of the unique role of the HER2 gene in diagnosis, therapy and prognosis of human breast cancer. In our previous research, a novel cyclic 2-helix small protein, MUT-DS, was discovered as an anti-...

journal_title：Amino acids

authors： Ren G,Webster JM,Liu Z,Zhang R,Miao Z,Liu H,Gambhir SS,Syud FA,Cheng Z

更新日期：2012-07-01 00:00:00

abstract：:Glucosinolates are amino acid-derived natural plant products found throughout the Capparales order. Glucosinolates and their degradation products have a wide range of biological activities, e.g. in plant defense as deterrents against insect and fungi. The conversion of amino acids to aldoximes is a key step in glucosi...

journal_title：Amino acids

authors： Mikkelsen MD,Petersen BL,Olsen CE,Halkier BA

更新日期：2002-01-01 00:00:00

abstract：:The cyclization of N-Boc-α-alkylserines to corresponding β-lactones under Mitsunobu reaction conditions and the ring opening with heterocyclic amines (pyrrolidine, piperidine, morpholine and thiomorpholine) produced N-Boc-α-alkyl-β-(sec-amino)alanines. The removal of the Boc group gives di-hydrochlorides of non-protei...

journal_title：Amino acids

authors： Olma A,Lasota A,Kudaj A

更新日期：2012-06-01 00:00:00

abstract：:Nitric oxide synthase (NOS) activities are responsible for the enzymatic conversion of L-arginine into NO and L-citrulline. Relatively low amounts of NO are produced in intestinal epithelial cells or are released from nerve endings. The effects of NO production are related to the maintenance of epithelial integrity an...

journal_title：Amino acids

authors： Guihot G,Guimbaud R,Bertrand V,Narcy-Lambare B,Couturier D,Duée PH,Chaussade S,Blachier F

更新日期：2000-01-01 00:00:00

abstract：:A growing body of evidence implicates endoplasmic reticulum (ER)-induced cellular dysfunction and apoptosis as important factors to a variety of diseases. In endothelial cells (ECs), the sulfur-containing amino acid homocysteine (Hcy) causes EC apoptosis and reactive oxygen species (ROS) generation through induction o...

journal_title：Amino acids

authors： Kil JS,Jeong SO,Chung HT,Pae HO

更新日期：2017-04-01 00:00:00

abstract：:The human organism is exposed to numerous processes that generate reactive oxygen species (ROS). ROS may directly or indirectly cause oxidative modification and damage of proteins. Protein oxidation is regarded as a crucial event in the pathogenesis of various diseases ranging from rheumatoid arthritis to Alzheimer's ...

journal_title：Amino acids

authors： Pietzsch J,Bergmann R,Wuest F,Pawelke B,Hultsch C,van den Hoff J

更新日期：2005-12-01 00:00:00

abstract：:Glutaryl-CoA dehydrogenase deficiency (GDD), which is one of the most frequent organic acid disorders, is characterized by a specific age- and regional-dependent neuropathology. We hypothesized that the distinct brain damage in GDD could be caused by the main pathologic metabolites, the organic acids glutaric (GA) and...

journal_title：Amino acids

authors： Kölker S,Ahlemeyer B,Krieglstein J,Hoffmann GF

更新日期：2000-01-01 00:00:00

abstract：:The article traces the development of research on the naturally occurring amino acid L-3,4-dihydroxyphenylalanine (L-dopa), from the first synthesis of its D,L racemate in 1911, and the isolation of its L-isomer from seedling of Vicia faba beans to the amino acid's successful application, from 1961 onward, as the most...

journal_title：Amino acids

authors： Hornykiewicz O

更新日期：2002-01-01 00:00:00

abstract：:Glutamate dehydrogenase (GDH) plays a crucial role in amino acid deamination and has been used as an inductor of nutrients metabolism. In this study, we cloned and analyzed the GDH cDNAs in diploids (red crucian carp), triploids and tetraploids and characterized their expression profiles upon dietary treatments. Resul...

journal_title：Amino acids

authors： Liu Z,Zhou Y,Liu S,Zhong H,Zhang C,kang X,Liu Y

更新日期：2012-12-01 00:00:00

abstract：:Vigabatrin is a GABA derivative (gamma-vinyl GABA) which inhibits irreversibly the enzyme activity of GABA transaminase and thus increased indirectly brain GABA concentrations. We have used body temperature assay to examine the effects of Vigabatrin on thermoregulation in intact rats. In order to understand the mechan...

journal_title：Amino acids

authors： Nikolov RP,Yakimova KS

更新日期：2011-05-01 00:00:00

abstract：:The activity of lysine decarboxylase was studied in 3-day-old soybean (Glycine max (L.) Meer cv. Sakai) seedlings also in relation to light conditions. Lysine decarboxylase activity was mainly localized in the roots and to a lesser extent in the hypocotyls and was detectable in both the soluble and particulate fractio...

journal_title：Amino acids

authors： Ohe M,Scoccianti V,Bagni N,Tassoni A,Matsuzaki S

更新日期：2009-01-01 00:00:00

abstract：:Amino acid networks (AANs) are undirected networks consisting of amino acid residues and their interactions in three-dimensional protein structures. The analysis of AANs provides novel insight into protein science, and several common amino acid network properties have revealed diverse classes of proteins. In this revi...

journal_title：Amino acids

authors： Yan W,Zhou J,Sun M,Chen J,Hu G,Shen B

更新日期：2014-06-01 00:00:00

abstract：:Dietary polyamines and amino acids (AAs) are crucial for human growth, development, reproduction, and health. However, the scientific literature shows large variations in polyamine and AA concentrations among major staple foods of plant origin, and there is a scarcity of information regarding their complete compositio...

journal_title：Amino acids

authors： Hou Y,He W,Hu S,Wu G

更新日期：2019-08-01 00:00:00

abstract：:Pseudomonas aeruginosa is particularly difficult to treat because it possesses a variety of resistance mechanisms and because it often forms biofilms. Antimicrobial peptides represent promising candidates for future templates of antibiotic-resistant bacterial infections due to their unique mechanism of antimicrobial a...

journal_title：Amino acids

authors： Xie J,Li Y,Li J,Yan Z,Wang D,Guo X,Zhang J,Zhang B,Mou L,Yang W,Jiang X

更新日期：2018-10-01 00:00:00

abstract：:D-Aspartate (D-Asp) is an endogenous substance in mammals. Degradation of D-Asp is carried out only by D-aspartate oxidase (DDO). We measured DDO activity in porcine tissues, and produced an anti-porcine DDO antibody to examine the cellular localization of DDO. All the tissues examined showed DDO activities, whereas t...

journal_title：Amino acids

authors： Yamamoto A,Tanaka H,Ishida T,Horiike K

更新日期：2011-07-01 00:00:00

abstract：:The hepatitis B core (HBc) protein has been used successfully in numerous experiments as a carrier for heterologous peptides. Folding and capsid formation of the chimeric proteins is not always achieved easily. In silico analyses were performed to provide further comprehension of the feasibility for predicting success...

journal_title：Amino acids

authors： Janssens ME,Geysen D,Broos K,De Goeyse I,Robbens J,Van Petegem F,Timmermans JP,Guisez Y

更新日期：2010-05-01 00:00:00

abstract：:Starting from a chiral 4-hydroxymethyl pyrrolidin-2-one, an isostere of α-methyl homoserine tethered on a γ-lactam ring was prepared exploiting a stereoselective acylation-methylation sequence, followed by Curtius rearrangement, and structural assignment was confirmed by n.O.e. experiments. By reverting the sequence, ...

journal_title：Amino acids

authors： Civitavecchia A,Martelli G,Orena M,Rinaldi S

更新日期：2014-04-01 00:00:00

abstract：:Taking a minimalistic approach in efforts to lower the cost for the development of new synthetic antimicrobial peptides, ultrashort cationic lipopeptides were designed to mimic the amphiphilic nature crucial for their activity but with only a very short peptide sequence ligated to a lipidic acid. Nine ultrashort catio...

journal_title：Amino acids

authors： Domalaon R,Yang X,O'Neil J,Zhanel GG,Mookherjee N,Schweizer F

更新日期：2014-11-01 00:00:00

abstract：:In this paper we report the complete separation of amino acids as DABS-derivatives using a 3µm Supelcosil LC-18 (25 cm × 2.1 mm I.D.) narrowbore column. The system described makes it possible to perform the analysis of DABS-amino acids with a sensitivity to the femtomole level. We have also studied the conditions nece...

journal_title：Amino acids

authors： Stocchi V,Palma F,Piccoli G,Biagiarelli B,Magnani M,Masat L,Cucchiarini L

更新日期：1992-10-01 00:00:00

abstract：:Functionalized collagen-mimetic peptides (CMPs) have been widely used in the preparation of collagen-related biomaterials. Among the reported results, the induced noncovalent interactions between the implanted functional groups or moieties were frequently the key elements to promote the self-assembly of small CMPs. In...

journal_title：Amino acids

authors： Huang PW,Chang JM,Horng JC

更新日期：2016-12-01 00:00:00

abstract：:We proposed that the combination of conjugated linoleic acid (CLA) and arginine would decrease adiposity by depressing lipid synthesis in liver and adipose tissues of growing pigs. Pigs were allotted to treatments in a 2 × 2 factorial design with two lipids (CLA or canola oil) and two amino acids [L-arginine or L-alan...

journal_title：Amino acids

authors： Go G,Wu G,Silvey DT,Choi S,Li X,Smith SB

更新日期：2012-10-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (TAT) protein, a member of the protein transduction domain (PTD) superfamily, can deliver heterologous proteins across most biomembranes without losing bioactivity. However, there is no report on whether the TAT core domain containing the ...

journal_title：Amino acids

authors： Wu Y,Ren C,Gao Y,Hou B,Chen T,Zhang C

更新日期：2010-08-01 00:00:00

abstract：:This study investigated the effect of agmatine (Agm) in proliferation of ovine trophecdoderm cells (oTr1) as well as the importance of the arginine decarboxylase (ADC) and agmatinase (AGMAT) alternative pathway for synthesis of polyamines in ovine conceptuses during the peri-implantation period of pregnancy. Morpholin...

journal_title：Amino acids

authors： Lenis YY,Elmetwally MA,Tang W,Satterfield C,Dunlap K,Wu G,Bazer FW

更新日期：2018-02-01 00:00:00

abstract：:The unusual basic amino acid, hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)-lysine], is a modified lysine with the addition of the 4-aminobutyl moiety from the polyamine spermidine. This naturally occurring amino acid is a product of a unique posttranslational modification that occurs in only one cellular protein, euk...

journal_title：Amino acids

authors： Park MH,Nishimura K,Zanelli CF,Valentini SR

更新日期：2010-02-01 00:00:00