Abstract:
:In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the regeneration of pre-purification tools could be money-saving. We applied an affinity chromatography kit to remove albumin and the immunoglobulin chains from plasma and propose a simple and effective technical procedure for the regeneration of these affinity columns.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Millioni R,Puricelli L,Iori E,Tessari Pdoi
10.1007/s00726-007-0556-6subject
Has Abstractpub_date
2008-04-01 00:00:00pages
507-9issue
3eissn
0939-4451issn
1438-2199journal_volume
34pub_type
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