Abstract:
:G-protein-coupled receptors (GPCRs) are cell surface receptors. The dynamic property of receptor-receptor interactions in GPCRs modulates the kinetics of G-protein signaling and stability. In the present work, the structural and dynamic study of A(2A)R-D(2)R interactions was carried to acquire the understanding of the A(2A)R-D(2)R receptor activation and deactivation process, facilitating the design of novel drugs and therapeutic target for Parkinson's disease. The structure-based features (Alpha, Beta, SurfAlpha, and SurfBeta; GapIndex, Leakiness and Gap Volume) and slow mode model (ENM) facilitated the prediction of kinetics (K (off), K (on), and K (d)) of A(2A)R-D(2)R interactions. The results demonstrated the correlation coefficient 0.294 for K (d) and K (on) and the correlation coefficient 0.635 for K (d) and K (off), and indicated stable interfacial contacts in the formation of heterodimer. The coulombic interaction involving the C-terminal tails of the A(2A)R and intracellular loops (ICLs) of D(2)R led to the formation of interfacial contacts between A(2A)R-D(2)R. The properties of structural dynamics, ENM and KFC server-based hot-spot analysis illustrated the stoichiometry of A(2A)R-D(2)R contact interfaces as dimer. The propensity of amino acid residues involved in A(2A)R-D(2)R interaction revealed the presence of positively (R, H and K) and negatively (E and D) charged structural motif of TMs and ICL3 of A(2A)R and D(2)R at interface of dimer contact. Essentially, in silico structural and dynamic study of A(2A)R-D(2)R interactions will provide the basic understanding of the A(2A)R-D(2)R interfacial contact surface for activation and deactivation processes, and could be used as constructive model to recognize the protein-protein interactions in receptor assimilations.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Prakash A,Luthra PMdoi
10.1007/s00726-012-1218-xsubject
Has Abstractpub_date
2012-10-01 00:00:00pages
1451-64issue
4eissn
0939-4451issn
1438-2199journal_volume
43pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::3-Fluorotyrosine fluorescence is quenched effectively by phosphate ions not only by a dynamic but also by a static mechanism owing to H-bond complex formation in ground state. 3-Fluorotyrosine pKa values both in the ground and first excited state (8.3 and 4, respectively) are appreciably lower than those of tyrosine, ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805830
更新日期:1993-10-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (TAT) protein, a member of the protein transduction domain (PTD) superfamily, can deliver heterologous proteins across most biomembranes without losing bioactivity. However, there is no report on whether the TAT core domain containing the ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0534-2
更新日期:2010-08-01 00:00:00
abstract::The putative translation factor eIF5A is essential for cell viability and is highly conserved from archaebacteria to mammals. This factor is the only cellular protein that undergoes an essential posttranslational modification dependent on the polyamine spermidine, called hypusination. This review focuses on the functi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-007-0533-0
更新日期:2007-08-01 00:00:00
abstract::This study investigated the effect of different sampling environments on hemolymph amino acid content of individual Drosophila melanogaster larvae. Hemolymph was collected from individual third instar larvae under cold-anesthetized, awake, and stress conditions. Qualitative and quantitative hemolymph amino acid analys...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0284-1
更新日期:2010-03-01 00:00:00
abstract::Taurine is a β-amino acid found in high concentrations in excitable tissues, including the heart. A significant reduction in myocardial taurine content leads to the development of a unique dilated, atrophic cardiomyopathy. One of the major functions of taurine in the heart is the regulation of the respiratory chain. H...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-2110-2
更新日期:2016-02-01 00:00:00
abstract::Detailed knowledge of the composition of protein complexes is crucial for the understanding of their structure and function; however, appropriate techniques for compositional analyses of complexes largely rely on elaborate tagging, immunoprecipitation, cross-linking and purification strategies. The proteasome is a pro...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1296-9
更新日期:2012-09-01 00:00:00
abstract::The focus has been on the development of methodology for stereoselective preparation of spiroannulated intermediates of the Schöllkopf chiron and further transformations to cyclic alpha-amino acids. The spiroannulations are effected by Ru(II)-catalysed ring-closing metathesis reactions, by Ru(II)- and Pd(0)-catalysed ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-007-0512-5
更新日期:2008-04-01 00:00:00
abstract::A procedure for the synthesis of enantiopure beta3-amino acids from proteinogenic alpha-amino acids, developed by our group a few years ago, has been modified to enable the production of C-2 fully deuterated, C-protected beta3-amino acids and, even more important, the synthesis of valuable deuterium labelled N(Boc)-pr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0384-0
更新日期:2007-01-01 00:00:00
abstract::Recently agmatine, decarboxylated arginine, has been shown to be an important biological compound in several animal tissues. This paper summarizes the known information regarding the transport of arginine, its decarboxylation and the effects of the agmatine formed mainly on NO and polyamine synthesis. ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-003-0030-z
更新日期:2004-02-01 00:00:00
abstract::S-adenosylmethionine (SAM), a major substrate in 1-C metabolism is a common precursor in the biosynthetic pathways of polyamines and ethylene, two important plant growth regulators, which exhibit opposing developmental effects, especially during fruit ripening. However, the flux of various substrates including SAM int...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1624-8
更新日期:2014-03-01 00:00:00
abstract::Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective analysis considering the amount of samples present in hospital archives, combined with pathological, clinical, and outcome information available for every sample. Although unlocking the proteome of these tissues is still a challenge,...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1494-0
更新日期:2013-08-01 00:00:00
abstract::Predicting the three-dimensional structure of proteins is a long-standing challenge of computational biology, as the structure (or lack of a rigid structure) is well known to determine a protein's function. Predicting relative solvent accessibility (RSA) of amino acids within a protein is a significant step towards re...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02767-6
更新日期:2019-09-01 00:00:00
abstract::Ornithine decarboxylase antizyme 1 (AZ1) is a major regulatory protein responsible for the regulation and degradation of ornithine decarboxylase (ODC). To better understand the role of AZ1 in polyamine metabolism and in modulating the response to anticancer polyamine analogues, a small interfering RNA strategy was use...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1030-z
更新日期:2012-02-01 00:00:00
abstract::Fibrosis, closely related to chronic various diseases, is a pathological process characterised by the accumulation of collagen (largely collagen type I). Non-invasive methods are necessary for the diagnosis and follow-up of fibrosis. This study aimed to develop a collagen-targeted probe for the molecular imaging of fi...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2328-7
更新日期:2017-01-01 00:00:00
abstract::The effect of dietary amino acid taurine on the liver function of chronic hepatitis patients was investigated. The 24 chronic hepatitis patients with 2-5 times over normal activities of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) were selected and equally divided into taurine treatment and contr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0565-5
更新日期:2008-08-01 00:00:00
abstract::Based on ubiquitous presence of large ionic motifs and clusters in proteins involved in gene transcription and protein synthesis, we analyzed the distribution of ionizable sidechains in a broad selection of proteins with regulatory, metabolic, structural and adhesive functions, in agonist, antagonist, toxin and antimi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-012-1418-4
更新日期:2012-12-01 00:00:00
abstract::α-Pharmacological conotoxins are among the most selective ligands of nicotinic acetylcholine receptors with typical cysteine frameworks. They are characterized by the intercysteine loop and classified into various subfamilies, such as α3/5 and α4/7 conotoxins. A novel α-conotoxin, Pu14a (DCPPHPVPGMHKCVCLKTC), with a d...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1093-x
更新日期:2012-07-01 00:00:00
abstract::Obesity, type 2 diabetes (T2DM) and cardiovascular disease (CVD) are the most common preventable causes of morbidity and mortality worldwide. They represent major public health threat to our society. Increasing prevalence of obesity and T2DM contributes to escalating morbidity and mortality from CVD and stroke. Carnos...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-016-2208-1
更新日期:2016-05-01 00:00:00
abstract::Glycyl-L-proline (gly-pro) is an end product of collagen metabolism that is further cleaved by prolidase (EC 3.4.13.9); the resulting proline molecules are recycled into collagen or other proteins. We postulated a relationship between defective gly-pro hydrolysis, increased collagen degradation and skin destruction. T...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01366930
更新日期:1999-01-01 00:00:00
abstract::The major goal of this review is a critical discussion of the literature data on asymmetric synthesis of α-amino acids via Michael addition reactions involving Ni(II)-complexes of amino acids. The material covered is divided into two conceptually different groups dealing with applications of: (a) Ni(II)-complexes of g...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1764-5
更新日期:2014-09-01 00:00:00
abstract::The kidney plays a key role in arginine metabolism. Arginine production is controlled by argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) which metabolize citrulline and aspartate to arginine and fumarate whereas arginine consumption is dependent on arginine:glycine amidinotransferase (GAT), which ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0897-z
更新日期:2012-04-01 00:00:00
abstract::Protein-DNA interaction plays an important role in many biological processes. The classical methods and the novel technologies advanced have been developed for the interaction of protein-DNA. Recent developments of these methods and research achievements have been reviewed in this paper. ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-012-1377-9
更新日期:2012-09-01 00:00:00
abstract::Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-D: - and all-L: -cupiennin 1a towards pro- and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0471-0
更新日期:2011-01-01 00:00:00
abstract::Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time exposure of UV irradiation decreases procollagen mRNA expression in dermis and that chronic UV irradiation decreases collagen amounts and induces wrinkle formation. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1059-z
更新日期:2012-06-01 00:00:00
abstract::The G protein-coupled receptor kinase (GRK) family consists of seven cytosolic serine/threonine (Ser/Thr) protein kinases, and among them, GRK2 is involved in the regulation of an enormous range of both G protein-coupled receptors (GPCRs) and non-GPCR substrates that participate in or regulate many critical cellular p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-020-02864-x
更新日期:2020-07-01 00:00:00
abstract::We proposed that the combination of conjugated linoleic acid (CLA) and arginine would decrease adiposity by depressing lipid synthesis in liver and adipose tissues of growing pigs. Pigs were allotted to treatments in a 2 × 2 factorial design with two lipids (CLA or canola oil) and two amino acids [L-arginine or L-alan...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1255-5
更新日期:2012-10-01 00:00:00
abstract::Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria....
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0331-7
更新日期:2003-01-01 00:00:00
abstract::Anticancer peptides (ACPs) are biologically anticancer active molecules that are produced by mammals, plants, insects and microorganisms. Here, a new peptide (TC22) with the amino acid sequence MTVVLLLIVLPLLGGVHSSGIL was identified and characterized from the beetle Tribolium castaneum. We found it inhibited the growth...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2666-8
更新日期:2019-02-01 00:00:00
abstract::Addition of poly(A) to hnRNA in the cell nucleus is a post-transcriptional event and is presumed to be brought about by a specific poly(A)polymerase. Since it is known that tryptophan rapidly increases the cytoplasmic levels of polyadenylated mRNA, it was of interest to investigate whether the essential amino acid, tr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805989
更新日期:1993-06-01 00:00:00
abstract::Starting from a chiral 4-hydroxymethyl pyrrolidin-2-one, an isostere of α-methyl homoserine tethered on a γ-lactam ring was prepared exploiting a stereoselective acylation-methylation sequence, followed by Curtius rearrangement, and structural assignment was confirmed by n.O.e. experiments. By reverting the sequence, ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1671-9
更新日期:2014-04-01 00:00:00