Abstract：
:The study examined maturation of preovulatory germinal vesicles oocytes (GV oocytes) induced by gonadotropic hormone PMSG in the inbred C57Bl/6J mice (viewed as a gold standard for diverse biomedical studies) as well as in the first generation hybrid C57Bl/6J×СВА/lac and СВА/lac×C57Bl/6J mice at various ages. The most effective donors of GV oocytes were СВА/lac×C57Bl/6J mice (F1 hybrids) yielding 25±2 oocyte/mouse. In contrast, a significantly smaller number of GV oocytes can be isolated from the ovaries of female C57Bl/6J or C57Bl/6J×СВА/lac mice under the same conditions. At this, the greatest number of GV oocytes (42±4) can be retrieved from the ovaries of immature hybrid СВА/lac×C57Bl/6J mice aged 4 weeks. These mice demonstrated the largest share of GV oocytes, which attained MII stage during in vitro culturing. The data conclude that F1 hybrid СВА/lac×C57Bl/6J mice can be viewed as a handy experimental source yielding a large number of GV oocytes capable of meiotic maturation in a culture.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Riabukha YA,Petrova DA,Zatsepina OVdoi
10.1007/s10517-019-04603-zsubject
Has Abstractpub_date
2019-09-01 00:00:00pages
702-705issue
5eissn
0007-4888issn
1573-8221pii
10.1007/s10517-019-04603-zjournal_volume
167pub_type
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