Abstract：
:Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate. The dispersion of values for 1 and 48 h exposure, obtained by MTT method, was greater than of the values obtained by neutral red absorption test.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Rusanov AL,Luzgina NG,Lisitsa AVdoi
10.1007/s10517-017-3785-zsubject
Has Abstractpub_date
2017-06-01 00:00:00pages
284-288issue
2eissn
0007-4888issn
1573-8221pii
10.1007/s10517-017-3785-zjournal_volume
163pub_type
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