Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic Application.

Abstract:

:We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates.

journal_name

Bull Exp Biol Med

authors

Chentsova EV,Konyushko OI,Makarov MS,Egorova NS,Zinov'ev MY,Borovkova NV

doi

10.1007/s10517-015-2915-8

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

168-72

issue

1

eissn

0007-4888

issn

1573-8221

journal_volume

159

pub_type

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