Effect of Ionophores on Activity of Na+,Cl-(HCO 3- )-ATPase.

Abstract:

:We studied the effects of ionophores on activity of Na+,Cl-(HCO 3- )-ATPase. The most significant effect on the activity of this enzyme was produced by protonophore 2,4-dinitrophenol. The effect of this drug largely depended on the cation and anion composition and pH of the incubation medium and its pH. Activity of Na+,Cl-(HCO 3- )-ATPase increased at neutral and weakly alkaline pH and decreased at pH below 6.5-6.7. In control animals (without histamine injection) with very weak or absent Na+,Cl-(HCO 3- )-ATPase activity, the observed effect of the protonophore on ATPase activity was also virtually absent. The stimulatory effect of other ionophores (monensine, valinomycin, and A23187) was significantly weaker and depended on pH of the incubation medium, its cationic and anionic composition, and concentration of these ionophores.

journal_name

Bull Exp Biol Med

authors

Yurkiv VA,Melikhov VI,Shubin VS

doi

10.1007/s10517-016-3504-1

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

763-766

issue

6

eissn

0007-4888

issn

1573-8221

pii

10.1007/s10517-016-3504-1

journal_volume

161

pub_type

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