Abstract：
:Fibroblasts isolated from skin biopsy specimens from patients with genetic forms of Parkinson's disease, carriers of mutations in LRRK2 and PARK2 genes, and from a healthy volunteer were reprogrammed using lentiviral vectors into induced pluripotent stem cells (iPSC). iPSC were differentiated into neuron-like cells using a cocktail of differentiation factors (N2, B27, and Noggin). The iPSC lines derived from patients with different mutations and from a healthy volunteer cultured under the same conditions were characterized by different proportion of neuronal precursors and differentiated neurons. Control Po2 line contained 56% precursors, while B15 line with LRRK2 gene mutation (G2019S) contained 35% precursor cells. Similar regularities were characteristic of Tr5 culture carrying compound heterozygous mutations in PARK2 gene (del202-203AG and IVS1+1G/A) and containing 4% neuronal precursors. Further comparative studies of iPSC carrying various mutations and comparison with normal human cells will help to understand the molecular pathogenesis of some genetic variants of Parkinson's disease.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Konovalova EV,Novosadova EV,Grivennikov IA,Illarioshkin SNdoi
10.1007/s10517-015-3072-9subject
Has Abstractpub_date
2015-10-01 00:00:00pages
772-5issue
6eissn
0007-4888issn
1573-8221pii
10.1007/s10517-015-3072-9journal_volume
159pub_type
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