Abstract：
:We analyzed the concentration of extracellular DNA and its fractions in the dynamics of uncomplicated pregnancy Thirty women with singleton pregnancy were examined. The concentration of total, maternal, and fetal cell-free DNA in maternal blood was measured at gestation weeks 11-14, 24-26, and 30-32. The level of total cell-free DNA was evaluated by measuring the concentration of RASSF1A gene using quantitative PCR analysis, the level of cell-free fetal DNA was assessed by determining the hypermethylated part of RASSF1A gene. The concentration of total cell-free DNA and cell-free maternal DNA did not change during the first half of pregnancy, but increased after 24-26 weeks. The level of cell-free fetal DNA increased from the first to the second and third trimester: 14.15 (2.32-36.25), 24.87 (6.29-129.32), and 32.62 (8.97-133.52) GE/ml (p<0.05), respectively. Our results characterize the dynamics of the content of cell-free DNA and its fractions during pregnancy, which should be taken into account when using cell-free DNA for prediction of placenta-associated complications.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Karapetyan АО,Baev ОR,Krasnyi АМ,Sadekova АА,Мullabaeva SМdoi
10.1007/s10517-018-4295-3subject
Has Abstractpub_date
2018-11-01 00:00:00pages
92-95issue
1eissn
0007-4888issn
1573-8221pii
10.1007/s10517-018-4295-3journal_volume
166pub_type
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doi：
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