Abstract:
BACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry. METHODS:A total of 97 pleural effusions including 54 benign effusions, 10 atypical effusions, and 33 malignant effusions, classified based on cytological diagnosis and etiology, were subjected to ELISA to measure protein level and to immunocytochemistry in cell blocks to determine CAIX expression. RESULTS:CAIX levels were significantly higher in the malignant group compared with the benign group. Receiver operating characteristic curve analysis of benign and malignant pleural effusion for CAIX indicated an area under the curve of 0.82 with a value of 1882 pg/dL as the best threshold for distinguishing benign from malignant effusions, which yields a sensitivity, specificity, and accuracy of 63.6%, 94.4%, and 82.8%, respectively. None of the benign effusion expressed CAIX by immunocytochemistry. Malignant effusion cell blocks expressed CAIX in 63.6% of cases with 100% specificity. Upon combination of CAIX level and immunocytochemistry to detect malignant pleural effusions, the sensitivity and accuracy increased to 81.8% and 89.7%, respectively. The CAIX level determined by ELISA and the CAIX expression detected by immunocytochemistry were positively correlated. In atypical effusions, 5 cases (50%) exhibited CAIX levels higher than the cutoff value or were highlighted by CAIX immunocytochemistry. CONCLUSIONS:ELISA and immunocytochemistry methods for determination of CAIX protein are potential additional tests for detection of malignant pleural effusions.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Liao ND,Lee WYdoi
10.1002/cncy.21191subject
Has Abstractpub_date
2012-08-25 00:00:00pages
269-75issue
4eissn
1934-662Xissn
1934-6638journal_volume
120pub_type
杂志文章abstract:BACKGROUND:The authors conducted an analysis of 2 telepathology systems with different resolutions to determine how resolution affects the pathologists' ability to provide preliminary diagnoses for fine-needle aspirations (FNA). METHODS:FNA cases evaluated by telepathology between February 1, 2011 and January 18, 2012...
journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:The sensitivity of conventional cytology for the detection of malignant cells in pleural effusion is insufficient. GLUT1 and CAIX are the hallmarks of metabolic change in cancer cells. The aim of this study was to evaluate the usefulness of GLUT1 and CAIX expression to the detection of cancer cells in pleura...
journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
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更新日期:2013-07-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,多中心研究
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更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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更新日期:2016-03-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Cancer cytopathology
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更新日期:2021-02-01 00:00:00
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
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