Abstract:
BACKGROUND:Fluorescence in situ hybridization (FISH) to identify specific DNA target sequences in the nuclei of nondividing cells of numerous solid neoplasms has contributed to the introduction of molecular cytogenetics as a useful adjunct to cytology, leading recently to the "marriage" of the 2 disciplines. Numerous cancer molecular markers can now be investigated using different technical approaches, at both the gene and expression levels, in biopsies of various suspected cancers, including differentiated thyroid carcinoma. The limited amount of bioptic material is often insufficient to carry out multiple tests, and optimizing handling of the biopsy is desirable. METHODS:We have developed a home-brew tetracolor break-apart probe able to simultaneously identify the 2 most common genetic alterations in differentiated thyroid carcinoma: RET/PTC variants in papillary thyroid carcinoma and PAX8/PPARg fusion and variants in follicular thyroid carcinoma. RESULTS:The probe had 100% specificity, 99.5% sensitivity, and ≥ 3% cutoff. The probe was tested on RET/PTC and PAX8/PPARg RT-PCR positive controls, and feasibility was assessed in 368 thyroid nodule fine-needle aspirations (FNA). In the latter analysis, 24 FNAs had split RET signal, and 9 had split PPARg signal. FISH analysis of available surgically removed nodules confirmed the sensitivity of FISH in detecting abnormal clones and oligoclones. CONCLUSIONS:The home-brew tetracolor probe showed high feasibility, optimizing the use of the biological material in relation to the available molecular tests and maximizing the FISH experimental and slide-scoring times. This probe may be considered an alternative to RT-PCR when recovery and quality of RNA amplification from FNA are insufficient.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Caria P,Frau DV,Dettori T,Boi F,Lai ML,Mariotti S,Vanni Rdoi
10.1002/cncy.21397subject
Has Abstractpub_date
2014-05-01 00:00:00pages
377-85issue
5eissn
1934-662Xissn
1934-6638journal_volume
122pub_type
杂志文章abstract:BACKGROUND:Tumor spread through air spaces (STAS), a significant prognostic indicator, has been described recently as a pattern of invasion in pulmonary carcinomas. However, questions remain regarding preoperative identification of STAS and whether it represents an in vivo phenomenon versus an ex vivo artifact. METHOD...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22243
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Although patients found to have atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesions (LSILs) on Papanicolaou (Pap) testing are treated conservatively, 5.2% to 18.8% of them progress to high-grade squamous intraepithelial lesions (HSILs). The objective o...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21615
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:The diagnosis of breast lesions is usually confirmed by fine-needle aspiration cytology (FNAC) or histological biopsy. Although there is increasing literature regarding the advantages and limitations of both modalities, there is no literature regarding the accuracy of these modalities for diagnosing breast l...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21308
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Much of the reluctance about using cytology specimens rather than histology specimens to assess programmed death ligand 1 (PD-L1) expression for guiding the use of immune modulating drugs in the management of non-small cell lung cancer (NSCLC) is based on the belief that the alcohol-based fixatives favored b...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22216
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:The use of topically applied hemostatic substances during surgery has become a common practice. In some cases, the material is not absorbed or induces a granulomatous reaction resulting in a pseudotumoral lesion. With imaging studies, it is not possible to differentiate this from a tumor recurrence or absces...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22194
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Fine-needle aspiration (FNA) is widely accepted in the preoperative management of salivary gland lesions. The proposed Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) aims to standardize reporting terminology. Studies regarding the risk of malignancy (ROM) for the proposed categories continu...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22016
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The Milan System for Reporting Salivary Gland Cytopathology is a tiered classification scheme that includes 6 diagnostic categories. Neoplasm, which is 1 of the 6 proposed categories, consists of benign neoplasm and neoplasm of uncertain malignant potential (NUMP). NUMP is reserved for a salivary gland neopl...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22002
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology category of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) includes fine-needle aspiration (FNA) specimens that cannot straightforwardly be classified as benign or malignant. To determine whether morphologi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21984
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:p16(INK4a) Is overexpressed in almost all precancerous and carcinomatous lesions of the uterine cervix, secondary to interference between high-risk human papillomaviruses (hr-HPVs) and the retinoblastoma gene product. Overexpression of p16(INK4a) has also been identified in patients with high-grade urothelia...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21462
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Adequacy assessment is one of the most controversial and overlooked components in the daily practice of cytopathology, because it is generally determined from limited samples. Because voided urine varies widely in terms of its volume and cellularity, there is little consensus about the proper role for these ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21634
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The workup of a malignant effusion usually requires immunostaining with a panel of markers. Although nuclear Wilms tumor 1 (WT1) expression is widely used to detect tumors of ovarian and mesothelial origin, it is less well known that WT1 is also expressed in the cytoplasm of melanomas and mesenchymal tumors....
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21439
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Liquid-based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lun...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22069
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:The recent revision in terminology, with noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) replacing noninvasive follicular variant of papillary thyroid carcinoma, has reclassified the clinically indolent tumor as nonmalignant. The objective of this study was to evaluate th...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21981
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Epithelioid sarcoma (ES) is a rare mesenchymal tumor that is divided into 2 types: classic and proximal. To the authors' knowledge, ES has been poorly studied in cytology, with fewer than 50 cases reported to date. The objective of the current study was to analyze the cytological and immunohistochemical info...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22052
更新日期:2018-11-01 00:00:00
abstract::The American Society of Cytopathology has provided guidelines for goals and objectives for cytopathology fellows. There are 90 Accreditation Council for Graduate Medical Education-accredited cytopathology fellowship training programs in the United States, each with its own unique curriculum designed to achieve these g...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21483
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Fluorescence in situ hybridization (FISH) is routinely used to help clarify atypical urinary cytology. However, to the authors' knowledge, little is known regarding the frequency of chromosomal aberrations in non-neoplastic conditions that could potentially lead to false-positive FISH results. The objective ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20171
更新日期:2011-12-25 00:00:00
abstract:BACKGROUND:New developments in the treatment of lung cancer have necessitated the further histologic and cytologic subtyping of nonsmall cell lung carcinomas. Thyroid transcription factor-1 (TTF-1) long has served as the predominant marker for demonstrating lung origin. However, it is also expressed in a variety of oth...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20108
更新日期:2010-12-25 00:00:00
abstract:BACKGROUND:The availability of suitable biospecimens is critical to the success of advanced genomic analyses. The objective of this study was to assess the sensitivity of fine-needle aspiration (FNA) compared with gross surgical sampling (GSS) from surgical specimens for the detection of somatic mutations in breast can...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21599
更新日期:2015-11-01 00:00:00
abstract::The practice of cytopathology has dramatically changed due to advances in genomics and information technology. Cytology laboratories have accordingly become increasingly dependent on pathology informatics support to meet the emerging demands of precision medicine. Pathology informatics deals with information technolog...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21833
更新日期:2017-06-01 00:00:00
abstract::In diagnostic cytology, the known site-specific false positive rates at various anatomical sites for the risk malignancy (ROM) when a confirmed malignant diagnosis is made are comparatively well documented. ROM figures for diagnostic cytology specimens may vary according to the anatomical site of the specimen, the exa...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22322
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Antigen retrieval, a crucial technique for immunostaining, is often carried out on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The role of antigen retrieval in immunostaining of ethanol-fixed smears remains unclear. The authors evaluated the effects of 2 common antigen retrieval procedures, hea...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21192
更新日期:2012-06-25 00:00:00
abstract:BACKGROUND:Mantle cell lymphoma (MCL) demonstrates cytologic features that overlap with those of other types of B-cell non-Hodgkin lymphomas (B-cell NHLs) containing small to medium-sized cells. The accurate diagnosis of MCL is important because MCL has relatively more aggressive biologic behavior and thus requires spe...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21465
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Patients with low-grade urothelial carcinoma (LGUC) are at risk of recurrence and must undergo lifelong surveillance. To date, cytology and cystoscopy are the gold standard for the detection of de novo and recurrent LGUC. The objective of the current study was is to further characterize the role of cytology ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21272
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:To the authors' knowledge, the diagnostic value of cell block (CB) as an adjunct to ThinPrep liquid-based cytology (LBC) of bronchial washing specimens in the detection and subclassification of pulmonary neoplasms has not been well evaluated. The objective of the current study was to evaluate the diagnostic ...
journal_title:Cancer cytopathology
pub_type: 杂志文章,随机对照试验
doi:10.1002/cncy.20181
更新日期:2012-04-25 00:00:00
abstract::Ocular cytology specimens are small, with limited options for a repeat biopsy. Appropriate handling of these specimens and triaging for ancillary testing can be taxing. In this article, the author reviews a selection of potentially challenging diagnoses and current common practices and methods used in diagnosing ocula...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22384
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Although thyroid fine-needle aspiration (TFNA) is an excellent test in evaluating thyroid nodules, there are occasionally false negatives (FN). The clinical impact and pathologic features of FN TFNA is understudied in the peer-reviewed literature. METHODS:A cohort of patients with thyroid cancer was separat...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21196
更新日期:2012-10-25 00:00:00
abstract:BACKGROUND:Angiomyolipoma (AML), typically composed of smooth muscle, vessels, and fat, is generally a benign tumor in the kidneys. However, it occasionally occurs in extrarenal sites and behaves like a malignant tumor. AML is uncommonly encountered in fine-needle aspiration (FNA) samples and can cause diagnostic diffi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21824
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:The newly unveiled Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) has proposed salivary gland neoplasm of uncertain malignant potential (SUMP) as an indeterminate category. The category is reserved for fine-needle aspiration (FNA) cases that are diagnostic of a salivary gland neoplasm but c...
journal_title:Cancer cytopathology
pub_type: 杂志文章,多中心研究
doi:10.1002/cncy.22066
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:The Paris System (TPS) for Reporting Urinary Cytology aims to standardize urine cytology reporting. Per TPS, the diagnosis of "suspicious for high-grade urothelial carcinoma (SHGUC)" is applied in cases that have few urothelial cells with severe atypia but are quantitatively insufficient for a diagnosis of "...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22357
更新日期:2021-02-01 00:00:00
abstract::Cervical cancer and anal cancer share many similarities including causation by oncogenic human papillomaviruses; however, significant differences exist in their epidemiology, risk factors, biologic behavior, management, and treatment. Although rare, the incidence of anal cancer is alarmingly high and continues to incr...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.20126
更新日期:2011-02-25 00:00:00