Abstract：
BACKGROUND:Fluorescence in situ hybridization (FISH) is routinely used to help clarify atypical urinary cytology. However, to the authors' knowledge, little is known regarding the frequency of chromosomal aberrations in non-neoplastic conditions that could potentially lead to false-positive FISH results. The objective of the current study was to evaluate the frequency of chromosomal aberrations in benign cells of the urinary tract using the UroVysion FISH test. METHODS:The authors analyzed 77 Papanicolaou-stained benign urine cytology specimens with reactive epithelial atypia using a FISH assay detecting the chromosomes 3, 7, and 17 and the gene locus 9p21. A positive test result was defined as an increased copy number of at least 2 chromosomes in ≥ 4 of 25 cells, or > 10 cells with a tetraploid or octaploid pattern, or homozygous or heterozygous deletion of 9p21 (≥ 12cells). RESULTS:FISH was positive in 27 of 77 bladder washings (35.1%) including 25 of 65 bladder washings (40.5%) and 2 of 15 voided urines (13.5%) from patients with irritative bladder (15 of 36 patients), a history of radiotherapy (7 of 12 patients), nonspecific cystitis (3 of 11 patients), hematuria (3 of 8 patients), and lithiasis (1 of 4 patients) . In 7 of 27 FISH-positive urothelial specimens, the positivity was solely due a polyploid pattern (tetraploid/octaploid pattern) in > 10 of the cells. CONCLUSIONS:Chromosomal aberrations can occur in reactive urothelial cells, with a tetraploid pattern being the most common. Even an aneuploid pattern of FISH signals does not always prove malignancy because it rarely occurs in reactive urothelial cells. Correlation of FISH results with cytomorphology and patient history is crucial to avoid false-positive diagnoses.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Tapia C,Glatz K,Obermann EC,Grilli B,Barascud A,Herzog M,Schönegg R,Savic S,Bubendorf Ldoi
10.1002/cncy.20171subject
Has Abstractpub_date
2011-12-25 00:00:00pages
404-10issue
6eissn
1934-662Xissn
1934-6638journal_volume
119pub_type
杂志文章abstract：BACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry. METHODS:A total of 97 pleural effusions including 54 b...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.21191
更新日期：2012-08-25 00:00:00
abstract：BACKGROUND:More accurate biomarkers in cervical cytology screening could reduce the number of women unnecessarily referred for biopsy. This study investigated the ability of p16/Ki-67 dual staining to predict high-grade cervical intraepithelial neoplasia (CIN) in human papillomavirus (HPV)-positive women from the Norwe...
journal_title：Cancer cytopathology
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更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:Second-opinion diagnosis (SOD) on pathological material is an accepted practice before definitive therapy is considered for referred patients. The thyroid gland is an anatomical site prone to diagnostic disagreement between pathologists. We performed a review of the literature that addressed the role of inte...
journal_title：Cancer cytopathology
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更新日期：2014-08-01 00:00:00
abstract：:Ocular cytology specimens are small, with limited options for a repeat biopsy. Appropriate handling of these specimens and triaging for ancillary testing can be taxing. In this article, the author reviews a selection of potentially challenging diagnoses and current common practices and methods used in diagnosing ocula...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.22384
更新日期：2020-11-02 00:00:00
abstract：BACKGROUND:The recent revision in terminology, with noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) replacing noninvasive follicular variant of papillary thyroid carcinoma, has reclassified the clinically indolent tumor as nonmalignant. The objective of this study was to evaluate th...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.21981
更新日期：2018-05-01 00:00:00
abstract：:Two-thirds of neuroendocrine neoplasms arising in the human body originate from the gastrointestinal system or pancreas. Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous, comprising both well differentiated neuroendocrine tumors (NETs) and poorly differentiated neuroendocrine carcinomas (NECs). The cl...
journal_title：Cancer cytopathology
pub_type： 杂志文章,评审
doi：10.1002/cncy.22073
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Morphologic and genetic analysis of thyroid nodules may be performed from a single vial. Preanalytic variables that affect nucleic acid extracted from a single vial are evaluated. METHODS:Thyroid fine-needle aspiration (FNA) specimens collected in CytoLyt were evaluated. A ThinPrep slide was prepared. Extra...
journal_title：Cancer cytopathology
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abstract：:BACKGROUND.: Pancreatic ductal adenocarcinoma is rarely detected early enough for patients to be cured. The objective of the authors was to develop a monoclonal antibody to distinguish adenocarcinoma and precancerous intraductal papillary mucinous neoplasia (IPMN) from benign epithelium. METHODS.: Mice were immunized ...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.21223
更新日期：2013-01-01 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2013-03-01 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2019-07-01 00:00:00
abstract：BACKGROUND:Fine-needle aspiration biopsy of spindled mesenchymal lesions, including schwannomas, are challenging because of overlapping cytomorphological features. The objective of the current study was to identify key diagnostic cytological criteria for the diagnosis of schwannoma and to distinguish it from its common...
journal_title：Cancer cytopathology
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更新日期：2015-03-01 00:00:00
abstract：BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology category of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) includes fine-needle aspiration (FNA) specimens that cannot straightforwardly be classified as benign or malignant. To determine whether morphologi...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.21984
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Liquid-based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lun...
journal_title：Cancer cytopathology
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更新日期：2018-12-01 00:00:00
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journal_title：Cancer cytopathology
pub_type： 杂志文章
doi：10.1002/cncy.20151
更新日期：2011-04-25 00:00:00
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journal_title：Cancer cytopathology
pub_type： 杂志文章
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更新日期：2021-02-01 00:00:00
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journal_title：Cancer cytopathology
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doi：10.1002/cncy.20097
更新日期：2010-10-25 00:00:00
abstract：:Cervical cancer and anal cancer share many similarities including causation by oncogenic human papillomaviruses; however, significant differences exist in their epidemiology, risk factors, biologic behavior, management, and treatment. Although rare, the incidence of anal cancer is alarmingly high and continues to incr...
journal_title：Cancer cytopathology
pub_type： 杂志文章,评审
doi：10.1002/cncy.20126
更新日期：2011-02-25 00:00:00
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journal_title：Cancer cytopathology
pub_type： 杂志文章
doi：10.1002/cncy.20166
更新日期：2011-12-25 00:00:00
abstract：BACKGROUND:The separation of benign from malignant mesothelial proliferations on effusion cytology can be difficult. Loss of methylthioadenosine phosphorylase (MTAP) by immunohistochemistry is an established marker of malignancy in mesothelial proliferations, but to the authors' knowledge largely has been applied only ...
journal_title：Cancer cytopathology
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doi：10.1002/cncy.22221
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:Fluorescent in situ hybridization (FISH) analysis of urine samples has proven to be a valuable adjunctive test to urine cytology for both diagnosis and monitoring recurrence of urothelial carcinoma. Automated FISH analysis has the potential to improve laboratory efficiency and to reduce interobserver and int...
journal_title：Cancer cytopathology
pub_type： 杂志文章
doi：10.1002/cncy.20145
更新日期：2011-08-25 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2021-01-01 00:00:00
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journal_title：Cancer cytopathology
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doi：10.1002/cncy.21272
更新日期：2013-07-01 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2015-08-01 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2014-12-01 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2020-05-01 00:00:00
abstract：BACKGROUND:The sensitivity of conventional cytology for the detection of malignant cells in pleural effusion is insufficient. GLUT1 and CAIX are the hallmarks of metabolic change in cancer cells. The aim of this study was to evaluate the usefulness of GLUT1 and CAIX expression to the detection of cancer cells in pleura...
journal_title：Cancer cytopathology
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更新日期：2011-10-25 00:00:00
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journal_title：Cancer cytopathology
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更新日期：2014-12-01 00:00:00
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更新日期：2010-06-25 00:00:00
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更新日期：2020-04-01 00:00:00
