Abstract:
BACKGROUND:Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors previously described that IHC expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM tissues. In the current study, the authors investigated whether a combination of MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in cell blocks obtained from pleural effusions. METHODS:The authors examined IHC expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS:MTAP and BAP1 IHC differentiated MPM from RMC with 100% specificity for both and sensitivities of 42.2% and 60.0%, respectively. The combination of MTAP and BAP1 IHC yielded a sensitivity of 77.8%, which was higher than that of BAP1 IHC alone or 9p21 FISH alone (62.2%). Moreover, a high degree of concordance was observed between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS:A combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to be a reliable and useful method for differentiating MPM cells from RMC and can be used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54-63. © 2017 American Cancer Society.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Kinoshita Y,Hida T,Hamasaki M,Matsumoto S,Sato A,Tsujimura T,Kawahara K,Hiroshima K,Oda Y,Nabeshima Kdoi
10.1002/cncy.21928subject
Has Abstractpub_date
2018-01-01 00:00:00pages
54-63issue
1eissn
1934-662Xissn
1934-6638journal_volume
126pub_type
杂志文章abstract::The 8th Annual National Molecular Cytopathology Meeting, held in Naples, Italy, on December 2 to 3, 2019, addressed updates in diagnostic cytopathology and molecular classifications and specifically focused on lung cancer biomarker testing in cytology samples. Lung cancer continues to be the most commonly diagnosed no...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22291
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology category of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) includes fine-needle aspiration (FNA) specimens that cannot straightforwardly be classified as benign or malignant. To determine whether morphologi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21984
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry. METHODS:A total of 97 pleural effusions including 54 b...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21191
更新日期:2012-08-25 00:00:00
abstract::BACKGROUND.: Pancreatic ductal adenocarcinoma is rarely detected early enough for patients to be cured. The objective of the authors was to develop a monoclonal antibody to distinguish adenocarcinoma and precancerous intraductal papillary mucinous neoplasia (IPMN) from benign epithelium. METHODS.: Mice were immunized ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21223
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The workup of a malignant effusion usually requires immunostaining with a panel of markers. Although nuclear Wilms tumor 1 (WT1) expression is widely used to detect tumors of ovarian and mesothelial origin, it is less well known that WT1 is also expressed in the cytoplasm of melanomas and mesenchymal tumors....
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21439
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:In pediatric patients, a cutaneous nodule is usually diagnosed by performing an excisional biopsy, but fine-needle cytology (FNC) is a safer and noninvasive diagnostic method widely used to obtain diagnostic specimens with little stress to the patient. The authors compared the ability of FNC and biopsy to di...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20132
更新日期:2011-04-25 00:00:00
abstract::Follicular cell-derived carcinomas of the thyroid gland comprise a heterogeneous group of malignant neoplasms of the thyroid gland with varied histologic appearance and molecular profiles. In most patients, these tumors represent relatively indolent neoplasms; however, certain subtypes/variants behave in an aggressive...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21417
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:The sensitivity of conventional cytology for the detection of malignant cells in pleural effusion is insufficient. GLUT1 and CAIX are the hallmarks of metabolic change in cancer cells. The aim of this study was to evaluate the usefulness of GLUT1 and CAIX expression to the detection of cancer cells in pleura...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20153
更新日期:2011-10-25 00:00:00
abstract:BACKGROUND:The current study was conducted to compare DNA yield, including normalization to nuclear area, DNA amplification functionality, and detection of KRAS mutations between matched fine-needle aspiration (FNA) specimens and pancreatic resections diagnostic of pancreatic ductal adenocarcinoma. METHODS:A retrospec...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21935
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:The diagnosis of breast lesions is usually confirmed by fine-needle aspiration cytology (FNAC) or histological biopsy. Although there is increasing literature regarding the advantages and limitations of both modalities, there is no literature regarding the accuracy of these modalities for diagnosing breast l...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21308
更新日期:2013-10-01 00:00:00
abstract::In diagnostic cytology, the known site-specific false positive rates at various anatomical sites for the risk malignancy (ROM) when a confirmed malignant diagnosis is made are comparatively well documented. ROM figures for diagnostic cytology specimens may vary according to the anatomical site of the specimen, the exa...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22322
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Fine-needle aspiration of the thyroid is a common procedure, with an established role in reducing unnecessary thyroid surgery and identifying neoplasms and malignancies. METHODS:The study evaluated 1558 responses in the American Society for Clinical Pathology (ASCP) Non-GYN Assessment program of aspirates o...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21440
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Undifferentiated carcinoma (UC) is a rare, aggressive subtype of endometrial carcinoma. Dedifferentiated carcinomas (DCs) are UCs associated with a component of well differentiated endometrioid carcinoma. The authors sought to describe the morphologic features of UCs and DCs in cytologic specimens. METHODS:...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22351
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:The Paris System for Reporting Urinary Cytology (TPS) defines clear morphologic criteria to classify urinary specimens into 7 diagnostic categories. According to TPS, a nuclear-to-cytoplasmic ratio (N:C ratio) >0.7 and hyperchromasia must be observed to render a diagnosis of high-grade urothelial carcinoma (...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22218
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:Current immunohistochemical and in situ hybridization (ISH) assays are generally inconclusive for clonality unless plasmacytic differentiation is present. This study examined a series of cytology specimens and explored the ability of a branched-chain RNA (bRNA) ISH assay for immunoglobulin κ constant (IGKC) ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21629
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Although thyroid fine-needle aspiration (TFNA) is an excellent test in evaluating thyroid nodules, there are occasionally false negatives (FN). The clinical impact and pathologic features of FN TFNA is understudied in the peer-reviewed literature. METHODS:A cohort of patients with thyroid cancer was separat...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21196
更新日期:2012-10-25 00:00:00
abstract:BACKGROUND:The separation of benign from malignant mesothelial proliferations on effusion cytology can be difficult. Loss of methylthioadenosine phosphorylase (MTAP) by immunohistochemistry is an established marker of malignancy in mesothelial proliferations, but to the authors' knowledge largely has been applied only ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22221
更新日期:2020-02-01 00:00:00
abstract::Two-thirds of neuroendocrine neoplasms arising in the human body originate from the gastrointestinal system or pancreas. Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous, comprising both well differentiated neuroendocrine tumors (NETs) and poorly differentiated neuroendocrine carcinomas (NECs). The cl...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22073
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:Limited studies have examined the impact of the Bethesda System for Reporting Thyroid Cytopathology (BSRTC) and specifically the category of atypia or follicular lesion of undetermined significance (AUS/FLUS). We studied their effects on reporting rates, subsequent management, and surgical outcome over a 10-...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21468
更新日期:2014-12-01 00:00:00
abstract::Cervical cancer and anal cancer share many similarities including causation by oncogenic human papillomaviruses; however, significant differences exist in their epidemiology, risk factors, biologic behavior, management, and treatment. Although rare, the incidence of anal cancer is alarmingly high and continues to incr...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.20126
更新日期:2011-02-25 00:00:00
abstract:BACKGROUND:The aim of this study was to validate clinical utilization of routinely prepared cytology specimens for molecular testing to detect EGFR or KRAS mutations in lung cancer. METHODS:From September 2009 to April 2010, the authors collected 209 cytology specimens from patients with lung cancer at the MD Anderson...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20151
更新日期:2011-04-25 00:00:00
abstract:BACKGROUND:Mantle cell lymphoma (MCL) demonstrates cytologic features that overlap with those of other types of B-cell non-Hodgkin lymphomas (B-cell NHLs) containing small to medium-sized cells. The accurate diagnosis of MCL is important because MCL has relatively more aggressive biologic behavior and thus requires spe...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21465
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:The use of topically applied hemostatic substances during surgery has become a common practice. In some cases, the material is not absorbed or induces a granulomatous reaction resulting in a pseudotumoral lesion. With imaging studies, it is not possible to differentiate this from a tumor recurrence or absces...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22194
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Angiomyolipoma (AML), typically composed of smooth muscle, vessels, and fat, is generally a benign tumor in the kidneys. However, it occasionally occurs in extrarenal sites and behaves like a malignant tumor. AML is uncommonly encountered in fine-needle aspiration (FNA) samples and can cause diagnostic diffi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21824
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:The objective of this study was to evaluate the diagnostic accuracy of the Bladder EpiCheck test in the follow-up of patients with non-muscle-invasive bladder cancer (NMIBC) and to compare it with the accuracy of urinary cytology, cystoscopy, and/or histology. METHODS:In total, 243 patients were enrolled in...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22152
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:Subtyping non-small cell lung carcinomas (NSCLC) into adenocarcinoma (ACA) or squamous cell carcinoma (SQCC) is important for treatment and specimen triage for molecular studies. To preserve tissue for molecular studies in cytology/small biopsy specimens, a 2-antibody cocktail for NSCLC subtyping was develop...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21707
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Transbronchial needle aspiration (TBNA) of peripheral lung nodules can be difficult with conventional devices due to their limited flexibility. A promising new technology for accessing these lesions is the PeriView FLEX TBNA device, which has a flexible spiral-grooved needle. The present study reports the un...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22240
更新日期:2020-05-01 00:00:00
abstract::An increasing number of renal cell carcinomas (RCCs) require ancillary studies for diagnosis. The majority of renal fine needle aspirates do not require ancillary studies. Among the most common useful stains are cytokeratin 7 (separating clear cell RCC [negative] from papillary RCC, clear cell papillary RCC, and multi...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22029
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Germline and somatic mutations of DICER1 have been identified in various types of neoplastic lesions, with germline DICER1 mutation being linked to autosomal dominant hereditary pleiotropic tumor syndrome (DICER1 syndrome). Patients with DICER1 syndrome are at increased risk of developing thyroid disease, in...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22329
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Fluorescent in situ hybridization (FISH) analysis of urine samples has proven to be a valuable adjunctive test to urine cytology for both diagnosis and monitoring recurrence of urothelial carcinoma. Automated FISH analysis has the potential to improve laboratory efficiency and to reduce interobserver and int...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20145
更新日期:2011-08-25 00:00:00