Abstract:
BACKGROUND:Fluorescent in situ hybridization (FISH) analysis of urine samples has proven to be a valuable adjunctive test to urine cytology for both diagnosis and monitoring recurrence of urothelial carcinoma. Automated FISH analysis has the potential to improve laboratory efficiency and to reduce interobserver and intraobserver variability, resulting in more accurate, reproducible, assay performance. METHODS:A total of 3200 slides containing urine specimens, hybridized with the UroVysion Bladder Cancer Kit (Abbott Molecular, Des Plaines, Illinois), a 4-probe set for chromosomes 3, 7, 17, and 9p21, was evaluated at Acupath Laboratories. The slides were analyzed over a 7-month period, using the Ikoniscope - oncoFISH bladder Test System (Ikonisys, New Haven, Connecticut). RESULTS:Analysis included the incorporation of a "flagging" system developed by Acupath Laboratories to identify cases, based on specific criteria, likely to benefit from further manual review. By using US Food and Drug Administration (FDA)-cleared scoring criteria, 96.3% of the slides could be reported directly from the automated scan, requiring no manual review of the slide. For the remaining 3.7% of the samples (all of which were very hypocellular), a manual review of each slide subsequently allowed diagnoses to be successfully reported. The average scan time was 31.7 minutes, and the average slide scan review time was 8.3 minutes. CONCLUSIONS:This study demonstrated the value of an automated approach to the analysis of FISH slides, affording the benefit of high-throughput while providing the user with the necessary images and tools to quickly and accurately report a case.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Marganski WA,El-Sirgany Costa V,Kilpatrick MW,Tafas T,Yim J,Matthews Mdoi
10.1002/cncy.20145subject
Has Abstractpub_date
2011-08-25 00:00:00pages
279-89issue
4eissn
1934-662Xissn
1934-6638journal_volume
119pub_type
杂志文章abstract:BACKGROUND:The objective of this study was to assess how atypical diagnostic category (ADC) is followed up, its outcomes, and the predictors that are associated with subsequent diagnosis of neoplasm/malignancy. METHODS:We reviewed pancreatic endoscopic ultrasound fine-needle aspiration (EUS-FNA) with ADC and compared ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21389
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:The current study was conducted to compare DNA yield, including normalization to nuclear area, DNA amplification functionality, and detection of KRAS mutations between matched fine-needle aspiration (FNA) specimens and pancreatic resections diagnostic of pancreatic ductal adenocarcinoma. METHODS:A retrospec...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21935
更新日期:2017-11-01 00:00:00
abstract::In diagnostic cytology, the known site-specific false positive rates at various anatomical sites for the risk malignancy (ROM) when a confirmed malignant diagnosis is made are comparatively well documented. ROM figures for diagnostic cytology specimens may vary according to the anatomical site of the specimen, the exa...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22322
更新日期:2021-01-01 00:00:00
abstract::Ocular cytology specimens are small, with limited options for a repeat biopsy. Appropriate handling of these specimens and triaging for ancillary testing can be taxing. In this article, the author reviews a selection of potentially challenging diagnoses and current common practices and methods used in diagnosing ocula...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22384
更新日期:2020-11-02 00:00:00
abstract::An increasing number of renal cell carcinomas (RCCs) require ancillary studies for diagnosis. The majority of renal fine needle aspirates do not require ancillary studies. Among the most common useful stains are cytokeratin 7 (separating clear cell RCC [negative] from papillary RCC, clear cell papillary RCC, and multi...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22029
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:New developments in the treatment of lung cancer have necessitated the further histologic and cytologic subtyping of nonsmall cell lung carcinomas. Thyroid transcription factor-1 (TTF-1) long has served as the predominant marker for demonstrating lung origin. However, it is also expressed in a variety of oth...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20108
更新日期:2010-12-25 00:00:00
abstract:BACKGROUND:The separation of benign from malignant mesothelial proliferations on effusion cytology can be difficult. Loss of methylthioadenosine phosphorylase (MTAP) by immunohistochemistry is an established marker of malignancy in mesothelial proliferations, but to the authors' knowledge largely has been applied only ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22221
更新日期:2020-02-01 00:00:00
abstract::Follicular cell-derived carcinomas of the thyroid gland comprise a heterogeneous group of malignant neoplasms of the thyroid gland with varied histologic appearance and molecular profiles. In most patients, these tumors represent relatively indolent neoplasms; however, certain subtypes/variants behave in an aggressive...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21417
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:To the authors' knowledge, the diagnostic value of cell block (CB) as an adjunct to ThinPrep liquid-based cytology (LBC) of bronchial washing specimens in the detection and subclassification of pulmonary neoplasms has not been well evaluated. The objective of the current study was to evaluate the diagnostic ...
journal_title:Cancer cytopathology
pub_type: 杂志文章,随机对照试验
doi:10.1002/cncy.20181
更新日期:2012-04-25 00:00:00
abstract:BACKGROUND:Antigen retrieval, a crucial technique for immunostaining, is often carried out on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The role of antigen retrieval in immunostaining of ethanol-fixed smears remains unclear. The authors evaluated the effects of 2 common antigen retrieval procedures, hea...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21192
更新日期:2012-06-25 00:00:00
abstract:BACKGROUND:Subtyping non-small cell lung carcinomas (NSCLC) into adenocarcinoma (ACA) or squamous cell carcinoma (SQCC) is important for treatment and specimen triage for molecular studies. To preserve tissue for molecular studies in cytology/small biopsy specimens, a 2-antibody cocktail for NSCLC subtyping was develop...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21707
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Current immunohistochemical and in situ hybridization (ISH) assays are generally inconclusive for clonality unless plasmacytic differentiation is present. This study examined a series of cytology specimens and explored the ability of a branched-chain RNA (bRNA) ISH assay for immunoglobulin κ constant (IGKC) ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21629
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The workup of a malignant effusion usually requires immunostaining with a panel of markers. Although nuclear Wilms tumor 1 (WT1) expression is widely used to detect tumors of ovarian and mesothelial origin, it is less well known that WT1 is also expressed in the cytoplasm of melanomas and mesenchymal tumors....
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21439
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Diagnosing giant cell-rich bone tumors can be challenging on limited biopsies. H3 histone family member 3A (H3F3A) (G34W/V/R/L) mutations are present in the majority of giant cell tumors (GCTs) of bone and H3 histone family member 3B (H3F3B) (K36M) mutations are present in nearly all chondroblastomas, but ar...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22000
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology category of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) includes fine-needle aspiration (FNA) specimens that cannot straightforwardly be classified as benign or malignant. To determine whether morphologi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21984
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:In pediatric patients, a cutaneous nodule is usually diagnosed by performing an excisional biopsy, but fine-needle cytology (FNC) is a safer and noninvasive diagnostic method widely used to obtain diagnostic specimens with little stress to the patient. The authors compared the ability of FNC and biopsy to di...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20132
更新日期:2011-04-25 00:00:00
abstract:BACKGROUND:Mantle cell lymphoma (MCL) demonstrates cytologic features that overlap with those of other types of B-cell non-Hodgkin lymphomas (B-cell NHLs) containing small to medium-sized cells. The accurate diagnosis of MCL is important because MCL has relatively more aggressive biologic behavior and thus requires spe...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21465
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Urine cytology has been used for screening of bladder cancer but has been limited by its low sensitivity. UroVysion is a multiprobe fluorescence in situ hybridization (FISH) assay that detects common chromosome abnormalities in bladder cancers. For this study, the authors evaluated the effectiveness of multi...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21327
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:The recent revision in terminology, with noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) replacing noninvasive follicular variant of papillary thyroid carcinoma, has reclassified the clinically indolent tumor as nonmalignant. The objective of this study was to evaluate th...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21981
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Fine-needle aspiration (FNA) is widely accepted in the preoperative management of salivary gland lesions. The proposed Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) aims to standardize reporting terminology. Studies regarding the risk of malignancy (ROM) for the proposed categories continu...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22016
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Accurate fine-needle aspiration (FNA) diagnosis of metastatic melanoma is of therapeutic and prognostic significance and often requires ancillary studies. To the authors' knowledge, the reliability of immunostaining using a pan-melanoma cocktail, Sry-related HMG-BOX gene 10 (SOX10), and microphthalmia transc...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21458
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:p16(INK4a) Is overexpressed in almost all precancerous and carcinomatous lesions of the uterine cervix, secondary to interference between high-risk human papillomaviruses (hr-HPVs) and the retinoblastoma gene product. Overexpression of p16(INK4a) has also been identified in patients with high-grade urothelia...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21462
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:The accumulation of cyclin-dependent kinase inhibitor 2A (p16ink4a ) protein in a cell is associated with neoplastic progression in precancerous cervical lesions. Dual staining for p16ink4a and Ki-67 has been proposed as a triage test in cervical cancer screening for women who test positive for human papillo...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21800
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors p...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21928
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Fluorescence in situ hybridization (FISH) to identify specific DNA target sequences in the nuclei of nondividing cells of numerous solid neoplasms has contributed to the introduction of molecular cytogenetics as a useful adjunct to cytology, leading recently to the "marriage" of the 2 disciplines. Numerous c...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21397
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:Mutations of AT-rich interactive domain 1 (ARID1A) have been associated with a worse outcome after intravesical treatment with bacille Calmette-Guérin in patients with non-muscle-invasive bladder cancer (NMIBC). Loss of ARID1A protein expression in urine cytology may serve as an indication of an ARID1A mutat...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22167
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:The use of topically applied hemostatic substances during surgery has become a common practice. In some cases, the material is not absorbed or induces a granulomatous reaction resulting in a pseudotumoral lesion. With imaging studies, it is not possible to differentiate this from a tumor recurrence or absces...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22194
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:The 2007 Bethesda classification for thyroid cytology defines follicular neoplasm as a category of cases with cellular specimens demonstrating abundant follicular cells arranged in a microfollicular pattern with little or no colloid. The current recommendation for the management of these cases is diagnostic ...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21425
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Much of the reluctance about using cytology specimens rather than histology specimens to assess programmed death ligand 1 (PD-L1) expression for guiding the use of immune modulating drugs in the management of non-small cell lung cancer (NSCLC) is based on the belief that the alcohol-based fixatives favored b...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22216
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:The usefulness of GATA3 (GATA-binding protein 3 to DNA sequence [A/T]GATA[A/G]) as a marker for metastatic breast carcinoma in serous effusion specimens was investigated. METHODS:Cell block sections from 74 serous effusion specimens (32 ascitic, 2 pericardial, and 40 pleural fluids) were stained with an ant...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21393
更新日期:2014-04-01 00:00:00