Abstract:
BACKGROUND:The Paris System for Reporting Urinary Cytology (TPS) defines clear morphologic criteria to classify urinary specimens into 7 diagnostic categories. According to TPS, a nuclear-to-cytoplasmic ratio (N:C ratio) >0.7 and hyperchromasia must be observed to render a diagnosis of high-grade urothelial carcinoma (HGUC). TPS was established using only liquid-based preparation techniques, and to the authors' knowledge it is unknown whether TPS can be applied using other preparation methods. METHODS:In the current prospective study, voided urine samples from patients with HGUC and negative for HGUC (NHGUC) were prepared using both ThinPrep and cytospin methods. ImageJ image processing software was used to measure the N:C ratio and hyperchromasia. For each patient, the N:C ratio and degree of hyperchromasia of urothelial cells present in both cytopreparations were compared. RESULTS:A total of 10 HGUC cases and 9 NHGUC cases, represented by a total of 688 cells (mean, 36.7 cells in HGUC cases; and mean, 35.8 cells in NHGUC cases), were evaluated in the current study. An overall comparison of HGUC cells with NHGUC cells demonstrated that HGUC cells had a higher average N:C ratio (0.5465 vs 0.2846) and greater hyperchromasia as measured by the average nuclear pixel gray value (100.8 vs 120.7). The N:C ratio was statistically different in 4 NHGUC cases, demonstrating higher N:C ratios in the ThinPrep preparations. Hyperchromasia was found to be statistically different in 6 cases, 5 of which demonstrated increased hyperchromasia in the ThinPrep specimens. CONCLUSIONS:The morphologic features of HGUC cells appear to be similar in samples prepared using the ThinPrep and cytospin methods, and therefore TPS criteria may be applied successfully in laboratories that use these methods.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Richardson CJ,Pambuccian SE,Barkan GAdoi
10.1002/cncy.22218subject
Has Abstractpub_date
2020-02-01 00:00:00pages
119-125issue
2eissn
1934-662Xissn
1934-6638journal_volume
128pub_type
杂志文章abstract:BACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry. METHODS:A total of 97 pleural effusions including 54 b...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21191
更新日期:2012-08-25 00:00:00
abstract::A pediatric cytopathology symposium was held at the recent 20th International Congress of Cytology, which convened in Sydney, Australia, in May 2019. This educational event brought together cytopathologists from different countries and different institutions to discuss some of the practical considerations when applyin...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22166
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Although thyroid fine-needle aspiration (TFNA) is an excellent test in evaluating thyroid nodules, there are occasionally false negatives (FN). The clinical impact and pathologic features of FN TFNA is understudied in the peer-reviewed literature. METHODS:A cohort of patients with thyroid cancer was separat...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21196
更新日期:2012-10-25 00:00:00
abstract:BACKGROUND:Mutations of AT-rich interactive domain 1 (ARID1A) have been associated with a worse outcome after intravesical treatment with bacille Calmette-Guérin in patients with non-muscle-invasive bladder cancer (NMIBC). Loss of ARID1A protein expression in urine cytology may serve as an indication of an ARID1A mutat...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22167
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Urine cytology results that are suspicious for urothelial carcinoma (UC) are challenging. The objective of this study was to elucidate the clinical significance of such results in patients who have a negative cystoscopy. METHODS:In this prospective study, 83 patients who had urine cytology that was suspicio...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22252
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors p...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21928
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The primary goal of this study was to compare the clinical performance of an optimized and rigorously controlled immunocytochemical (ICC) assay for p16(INK4a) to high-risk (HR) human papillomavirus (HPV) detection by polymerase chain reaction (PCR) as diagnostic adjuncts for cytology specimens from colposcop...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20078
更新日期:2010-06-25 00:00:00
abstract:BACKGROUND:Germline and somatic mutations of DICER1 have been identified in various types of neoplastic lesions, with germline DICER1 mutation being linked to autosomal dominant hereditary pleiotropic tumor syndrome (DICER1 syndrome). Patients with DICER1 syndrome are at increased risk of developing thyroid disease, in...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22329
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:High-risk human papillomavirus (hrHPV) DNA detection is generally accepted for the triage of women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ASC-US). However, no consensus has been reached on the optimal management of low-grade squamous intraepithelial lesions (LSIL)...
journal_title:Cancer cytopathology
pub_type: 杂志文章,meta分析,评审
doi:10.1002/cncy.21325
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Current immunohistochemical and in situ hybridization (ISH) assays are generally inconclusive for clonality unless plasmacytic differentiation is present. This study examined a series of cytology specimens and explored the ability of a branched-chain RNA (bRNA) ISH assay for immunoglobulin κ constant (IGKC) ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21629
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Ewing sarcoma (ES) is a round cell sarcoma that can be challenging to diagnose on cytologic material given its significant overlap with numerous mesenchymal, epithelial, and lymphoid cytomorphologic mimics. The objective of this study was to assess the utility of a novel marker, NKX2.2, in the diagnosis of E...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22056
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Liquid-based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lun...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22069
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:The Papanicolaou Society of Cytopathology has recently published a new proposal for pancreaticobiliary cytology terminology. For the current study, the authors applied this new classification for pancreaticobiliary cytology to a set of previously diagnosed endoscopic ultrasound (EUS)-guided fine-needle aspir...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21559
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Fine-needle aspiration biopsy of spindled mesenchymal lesions, including schwannomas, are challenging because of overlapping cytomorphological features. The objective of the current study was to identify key diagnostic cytological criteria for the diagnosis of schwannoma and to distinguish it from its common...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21506
更新日期:2015-03-01 00:00:00
abstract::Cervical cancer and anal cancer share many similarities including causation by oncogenic human papillomaviruses; however, significant differences exist in their epidemiology, risk factors, biologic behavior, management, and treatment. Although rare, the incidence of anal cancer is alarmingly high and continues to incr...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.20126
更新日期:2011-02-25 00:00:00
abstract::Two-thirds of neuroendocrine neoplasms arising in the human body originate from the gastrointestinal system or pancreas. Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous, comprising both well differentiated neuroendocrine tumors (NETs) and poorly differentiated neuroendocrine carcinomas (NECs). The cl...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22073
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:The objective of this study was to evaluate the diagnostic accuracy of the Bladder EpiCheck test in the follow-up of patients with non-muscle-invasive bladder cancer (NMIBC) and to compare it with the accuracy of urinary cytology, cystoscopy, and/or histology. METHODS:In total, 243 patients were enrolled in...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22152
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:The authors conducted an analysis of 2 telepathology systems with different resolutions to determine how resolution affects the pathologists' ability to provide preliminary diagnoses for fine-needle aspirations (FNA). METHODS:FNA cases evaluated by telepathology between February 1, 2011 and January 18, 2012...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21404
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:The separation of benign from malignant mesothelial proliferations on effusion cytology can be difficult. Loss of methylthioadenosine phosphorylase (MTAP) by immunohistochemistry is an established marker of malignancy in mesothelial proliferations, but to the authors' knowledge largely has been applied only ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22221
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:The availability of suitable biospecimens is critical to the success of advanced genomic analyses. The objective of this study was to assess the sensitivity of fine-needle aspiration (FNA) compared with gross surgical sampling (GSS) from surgical specimens for the detection of somatic mutations in breast can...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21599
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:The sensitivity of conventional cytology for the detection of malignant cells in pleural effusion is insufficient. GLUT1 and CAIX are the hallmarks of metabolic change in cancer cells. The aim of this study was to evaluate the usefulness of GLUT1 and CAIX expression to the detection of cancer cells in pleura...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20153
更新日期:2011-10-25 00:00:00
abstract:BACKGROUND:Morphologic and genetic analysis of thyroid nodules may be performed from a single vial. Preanalytic variables that affect nucleic acid extracted from a single vial are evaluated. METHODS:Thyroid fine-needle aspiration (FNA) specimens collected in CytoLyt were evaluated. A ThinPrep slide was prepared. Extra...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22270
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Second-opinion diagnosis (SOD) on pathological material is an accepted practice before definitive therapy is considered for referred patients. The thyroid gland is an anatomical site prone to diagnostic disagreement between pathologists. We performed a review of the literature that addressed the role of inte...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21436
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Molecular testing to determine gene mutation status is now the recommended standard of care for patients with advanced or metastatic Non-small cell lung cancer (NSCLC). Because the majority of patients with NSCLC present with metastatic disease, minimally invasive procedures are necessary for diagnosis, stag...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21513
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:The usefulness of GATA3 (GATA-binding protein 3 to DNA sequence [A/T]GATA[A/G]) as a marker for metastatic breast carcinoma in serous effusion specimens was investigated. METHODS:Cell block sections from 74 serous effusion specimens (32 ascitic, 2 pericardial, and 40 pleural fluids) were stained with an ant...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21393
更新日期:2014-04-01 00:00:00
abstract::The American Society for Colposcopy and Cervical Pathology (ASCCP) guidelines for the management of women with abnormal cervical screening test results is a powerful, evidence-based document representing an advance in the consistent treatment of women with cervical abnormalities. Nevertheless, the complexity of the gu...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20097
更新日期:2010-10-25 00:00:00
abstract:BACKGROUND:Diagnosing giant cell-rich bone tumors can be challenging on limited biopsies. H3 histone family member 3A (H3F3A) (G34W/V/R/L) mutations are present in the majority of giant cell tumors (GCTs) of bone and H3 histone family member 3B (H3F3B) (K36M) mutations are present in nearly all chondroblastomas, but ar...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22000
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The newly unveiled Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) has proposed salivary gland neoplasm of uncertain malignant potential (SUMP) as an indeterminate category. The category is reserved for fine-needle aspiration (FNA) cases that are diagnostic of a salivary gland neoplasm but c...
journal_title:Cancer cytopathology
pub_type: 杂志文章,多中心研究
doi:10.1002/cncy.22066
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Personalized oncology requires molecular analysis of tumor cells. Several studies have demonstrated that cytological material is suitable for DNA analysis, but to the authors' knowledge there are no systematic studies comparing how the yield and quality of extracted DNA is affected by the various techniques ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21276
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:The Milan System for Reporting Salivary Gland Cytopathology is a tiered classification scheme that includes 6 diagnostic categories. Neoplasm, which is 1 of the 6 proposed categories, consists of benign neoplasm and neoplasm of uncertain malignant potential (NUMP). NUMP is reserved for a salivary gland neopl...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22002
更新日期:2018-07-01 00:00:00