Abstract:
BACKGROUND:Morphologic and genetic analysis of thyroid nodules may be performed from a single vial. Preanalytic variables that affect nucleic acid extracted from a single vial are evaluated. METHODS:Thyroid fine-needle aspiration (FNA) specimens collected in CytoLyt were evaluated. A ThinPrep slide was prepared. Extracted nucleic acids were analyzed using Oncomine Comprehensive Panel, version 2, after Ion AmpliSeq library preparation. A pathologist and a cytotechnologist enumerated specimen cellularity. RESULTS:Fifty-six samples were collected from 55 nodules in 53 patients. Bethesda category correlated with cellularity (P = .01), and storage time (median, 43 days; range, 7-77 days) was longer for specimens in categories II and III than for those in categories IV and VI (P = .01). The mean specimen DNA concentration was 4.5 ng/µL (range, 0-23.8 ng/µL), and 25 (45%) had concentrations >3.3 ng/µL. The mean specimen RNA concentration was 4.8 ng/µL (range, 0-42.4 ng/µL), and 31 (55%) had concentrations >1.4 ng/µL. Nucleic acid quantity increased with epithelial cellularity. Storage time weakly correlated with the quantity of extracted DNA, independent of cellularity, but not extracted RNA. Greater proportions of cell-free DNA and lesser proportions of long, intact RNA fragments were extracted from a subset of samples with longer storage time. Among 15 single nucleotide variants, the median mutant allelic fraction was 15.1%. One false-negative result was identified. Five specimens subsequently determined to harbor a genetic alteration failed quality metrics. CONCLUSIONS:Cellularity and storage time affect the quantity and quality of nucleic acid extracted from thyroid FNA specimens collected in CytoLyt. Further investigation will serve to quantify the magnitude of such effects and to elucidate other contributing factors.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Heymann JJ,Yoxtheimer LM,Park HJ,Fernandez EM,Facey KE,Alperstein SA,Tran HV,Baek I,Scognamiglio T,Rennert H,Siddiqui MT,Song Wdoi
10.1002/cncy.22270subject
Has Abstractpub_date
2020-09-01 00:00:00pages
656-672issue
9eissn
1934-662Xissn
1934-6638journal_volume
128pub_type
杂志文章abstract:BACKGROUND:The use of topically applied hemostatic substances during surgery has become a common practice. In some cases, the material is not absorbed or induces a granulomatous reaction resulting in a pseudotumoral lesion. With imaging studies, it is not possible to differentiate this from a tumor recurrence or absces...
journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Although thyroid fine-needle aspiration (TFNA) is an excellent test in evaluating thyroid nodules, there are occasionally false negatives (FN). The clinical impact and pathologic features of FN TFNA is understudied in the peer-reviewed literature. METHODS:A cohort of patients with thyroid cancer was separat...
journal_title:Cancer cytopathology
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abstract:BACKGROUND:Fluorescence in situ hybridization (FISH) to identify specific DNA target sequences in the nuclei of nondividing cells of numerous solid neoplasms has contributed to the introduction of molecular cytogenetics as a useful adjunct to cytology, leading recently to the "marriage" of the 2 disciplines. Numerous c...
journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21951
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21389
更新日期:2014-06-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22322
更新日期:2021-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20145
更新日期:2011-08-25 00:00:00
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pub_type: 杂志文章,评审
doi:10.1002/cncy.20126
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journal_title:Cancer cytopathology
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pub_type: 杂志文章,多中心研究
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2016-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章,meta分析,评审
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更新日期:2013-12-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2012-08-25 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
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