Abstract:
BACKGROUND:Ewing sarcoma (ES) is a round cell sarcoma that can be challenging to diagnose on cytologic material given its significant overlap with numerous mesenchymal, epithelial, and lymphoid cytomorphologic mimics. The objective of this study was to assess the utility of a novel marker, NKX2.2, in the diagnosis of ES in cytologic material and its ability to distinguish ES from its mimics. METHODS:NKX2.2 immunohistochemistry was performed on cell blocks from 107 fine-needle aspirations, and nuclear expression was scored semiquantitatively for extent and intensity. The study cohort included ES (n = 10), well differentiated neuroendocrine tumor (n = 20), melanoma (n = 11), Merkel cell carcinoma (n = 10), small cell carcinoma (n = 10), alveolar rhabdomyosarcoma (n = 2), spindle cell/sclerosing rhabdomyosarcoma (n = 2), synovial sarcoma (n = 12), solitary fibrous tumor (n = 2), chronic lymphocytic leukemia (n = 10), lymphoblastic lymphoma (n = 11), adenoid cystic carcinoma (n = 6), and CIC-rearranged sarcoma (n = 1). RESULTS:NKX2.2 had high sensitivity (100%) and moderate specificity (85%) for the diagnosis of ES in cytologic material. NKX2.2 expression also was present in a subset of mesenchymal and epithelial mimics, and staining was most commonly observed in small cell carcinoma (80%) and well differentiated neuroendocrine tumor (45%). Among mesenchymal mimics, 42% exhibited NKX2.2 expression. NKX2.2 staining was absent in melanoma, adenoid cystic carcinoma, and lymphoproliferative neoplasms. CONCLUSIONS:NKX2.2 is a highly sensitive but only moderately specific marker for ES. Neuroendocrine neoplasms exhibit variable NKX2.2 expression and remain a significant potential diagnostic pitfall. Thus, NKX2.2 expression should be interpreted in the context of an appropriate immunohistochemical panel (and often with confirmatory molecular testing) for the accurate diagnosis of ES.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Russell-Goldman E,Hornick JL,Qian X,Jo VYdoi
10.1002/cncy.22056subject
Has Abstractpub_date
2018-11-01 00:00:00pages
942-949issue
11eissn
1934-662Xissn
1934-6638journal_volume
126pub_type
杂志文章abstract:BACKGROUND:The aim of this study was to validate clinical utilization of routinely prepared cytology specimens for molecular testing to detect EGFR or KRAS mutations in lung cancer. METHODS:From September 2009 to April 2010, the authors collected 209 cytology specimens from patients with lung cancer at the MD Anderson...
journal_title:Cancer cytopathology
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journal_title:Cancer cytopathology
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pub_type: 杂志文章,随机对照试验
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更新日期:2012-04-25 00:00:00
abstract:BACKGROUND:The Bethesda System for Reporting Thyroid Cytopathology category of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) includes fine-needle aspiration (FNA) specimens that cannot straightforwardly be classified as benign or malignant. To determine whether morphologi...
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更新日期:2018-05-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1002/cncy.21483
更新日期:2014-12-01 00:00:00
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doi:10.1002/cncy.21800
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journal_title:Cancer cytopathology
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更新日期:2014-07-01 00:00:00
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journal_title:Cancer cytopathology
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更新日期:2019-12-01 00:00:00
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journal_title:Cancer cytopathology
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更新日期:2014-06-01 00:00:00
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journal_title:Cancer cytopathology
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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