Abstract:
BACKGROUND:More accurate biomarkers in cervical cytology screening could reduce the number of women unnecessarily referred for biopsy. This study investigated the ability of p16/Ki-67 dual staining to predict high-grade cervical intraepithelial neoplasia (CIN) in human papillomavirus (HPV)-positive women from the Norwegian Cervical Cancer Screening Program. METHODS:Automated p16/Ki-67 dual staining was performed on liquid-based cytology samples from 266 women who were HPV-positive at their secondary screening. At a mean of 184 days after p16/Ki-67 staining, 201 women had a valid staining result and a conclusive follow-up diagnosis (histological diagnosis or HPV-negative diagnosis with normal cytology findings). The sensitivity and specificity for predicting the follow-up diagnosis were compared for cytology, p16/Ki-67 dual staining, and their combination. RESULTS:Sixty-seven percent of the study sample was p16/Ki-67-positive. The sensitivity of p16/Ki-67 staining for predicting CIN-2/3 was statistically significantly higher than the sensitivity of cytology (0.88 vs 0.79; P = .008), but this was not true for the prediction of CIN-3 (0.94 vs 0.88; P = .23). The specificity of cytology for predicting CIN-3 was significantly higher than the specificity of p16/Ki-67 staining (0.35 vs 0.28; P = .002), but this was not true for CIN-2/3 (0.35 vs 0.31; P = .063). For predicting CIN-2/3 and CIN-3, combination testing gave potentially better sensitivity (0.95 and 0.96, respectively) and better specificity (0.49 and 0.50, respectively). CONCLUSIONS:In a population of HPV-positive women, p16/Ki-67 dual staining was more sensitive but less specific than cytology for predicting high-grade CIN. The advantage of using both tests in different combinations is the potential for increasing the specificity or sensitivity in comparison with both methods performed individually. Cancer Cytopathol 2017;125:283-291. © 2016 American Cancer Society.
journal_name
Cancer Cytopatholjournal_title
Cancer cytopathologyauthors
Ovestad IT,Dalen I,Hansen E,Loge JL,Dybdahl BM,Dirdal MB,Moltu P,Berland JMdoi
10.1002/cncy.21807subject
Has Abstractpub_date
2017-04-01 00:00:00pages
283-291issue
4eissn
1934-662Xissn
1934-6638journal_volume
125pub_type
杂志文章abstract:BACKGROUND:The Paris System for Reporting Urinary Cytology (TPS) defines clear morphologic criteria to classify urinary specimens into 7 diagnostic categories. According to TPS, a nuclear-to-cytoplasmic ratio (N:C ratio) >0.7 and hyperchromasia must be observed to render a diagnosis of high-grade urothelial carcinoma (...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22218
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry. METHODS:A total of 97 pleural effusions including 54 b...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21191
更新日期:2012-08-25 00:00:00
abstract::BACKGROUND.: Pancreatic ductal adenocarcinoma is rarely detected early enough for patients to be cured. The objective of the authors was to develop a monoclonal antibody to distinguish adenocarcinoma and precancerous intraductal papillary mucinous neoplasia (IPMN) from benign epithelium. METHODS.: Mice were immunized ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21223
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The sensitivity of conventional cytology for the detection of malignant cells in pleural effusion is insufficient. GLUT1 and CAIX are the hallmarks of metabolic change in cancer cells. The aim of this study was to evaluate the usefulness of GLUT1 and CAIX expression to the detection of cancer cells in pleura...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20153
更新日期:2011-10-25 00:00:00
abstract:BACKGROUND:Limited studies have examined the impact of the Bethesda System for Reporting Thyroid Cytopathology (BSRTC) and specifically the category of atypia or follicular lesion of undetermined significance (AUS/FLUS). We studied their effects on reporting rates, subsequent management, and surgical outcome over a 10-...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21468
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Antigen retrieval, a crucial technique for immunostaining, is often carried out on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The role of antigen retrieval in immunostaining of ethanol-fixed smears remains unclear. The authors evaluated the effects of 2 common antigen retrieval procedures, hea...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21192
更新日期:2012-06-25 00:00:00
abstract:BACKGROUND:Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors p...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21928
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The objective of the current study was to investigate the clinical performance of detecting high-grade lesions with the CINtec PLUS p16(INK4a)/Ki-67 dual stain and the APTIMA human papillomavirus (HPV) Assay in a cohort of women with low-grade squamous intraepithelial lesion (LSIL) cytology. The authors also...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21233
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Undifferentiated carcinoma (UC) is a rare, aggressive subtype of endometrial carcinoma. Dedifferentiated carcinomas (DCs) are UCs associated with a component of well differentiated endometrioid carcinoma. The authors sought to describe the morphologic features of UCs and DCs in cytologic specimens. METHODS:...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22351
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:High-risk human papillomavirus (hrHPV) DNA detection is generally accepted for the triage of women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ASC-US). However, no consensus has been reached on the optimal management of low-grade squamous intraepithelial lesions (LSIL)...
journal_title:Cancer cytopathology
pub_type: 杂志文章,meta分析,评审
doi:10.1002/cncy.21325
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:The separation of benign from malignant mesothelial proliferations on effusion cytology can be difficult. Loss of methylthioadenosine phosphorylase (MTAP) by immunohistochemistry is an established marker of malignancy in mesothelial proliferations, but to the authors' knowledge largely has been applied only ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22221
更新日期:2020-02-01 00:00:00
abstract::Ocular cytology specimens are small, with limited options for a repeat biopsy. Appropriate handling of these specimens and triaging for ancillary testing can be taxing. In this article, the author reviews a selection of potentially challenging diagnoses and current common practices and methods used in diagnosing ocula...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22384
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:In the United States, the rate of cervical cancer is disproportionally higher in Hispanic and Black women compared with White women. In the current study, the authors compared human papillomavirus (HPV) testing and cytology results among Black and White women over a 24-month period. They then assessed the ra...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22316
更新日期:2020-11-01 00:00:00
abstract::The evaluation of mediastinal and hilar lymph nodes for tissue diagnosis and staging of lung cancer is now commonly performed by minimally invasive, nonsurgical procedures such as computed tomography-guided fine-needle aspiration and endobronchial ultrasound-guided transbronchial needle aspiration. Ensuring that a suf...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20166
更新日期:2011-12-25 00:00:00
abstract:BACKGROUND:The current study was conducted to compare DNA yield, including normalization to nuclear area, DNA amplification functionality, and detection of KRAS mutations between matched fine-needle aspiration (FNA) specimens and pancreatic resections diagnostic of pancreatic ductal adenocarcinoma. METHODS:A retrospec...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21935
更新日期:2017-11-01 00:00:00
abstract::A pediatric cytopathology symposium was held at the recent 20th International Congress of Cytology, which convened in Sydney, Australia, in May 2019. This educational event brought together cytopathologists from different countries and different institutions to discuss some of the practical considerations when applyin...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22166
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Epithelioid sarcoma (ES) is a rare mesenchymal tumor that is divided into 2 types: classic and proximal. To the authors' knowledge, ES has been poorly studied in cytology, with fewer than 50 cases reported to date. The objective of the current study was to analyze the cytological and immunohistochemical info...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.22052
更新日期:2018-11-01 00:00:00
abstract::The American Society of Cytopathology has provided guidelines for goals and objectives for cytopathology fellows. There are 90 Accreditation Council for Graduate Medical Education-accredited cytopathology fellowship training programs in the United States, each with its own unique curriculum designed to achieve these g...
journal_title:Cancer cytopathology
pub_type: 杂志文章,评审
doi:10.1002/cncy.21483
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:The workup of a malignant effusion usually requires immunostaining with a panel of markers. Although nuclear Wilms tumor 1 (WT1) expression is widely used to detect tumors of ovarian and mesothelial origin, it is less well known that WT1 is also expressed in the cytoplasm of melanomas and mesenchymal tumors....
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21439
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Morphologic and genetic analysis of thyroid nodules may be performed from a single vial. Preanalytic variables that affect nucleic acid extracted from a single vial are evaluated. METHODS:Thyroid fine-needle aspiration (FNA) specimens collected in CytoLyt were evaluated. A ThinPrep slide was prepared. Extra...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22270
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:The Papanicolaou Society of Cytopathology has recently published a new proposal for pancreaticobiliary cytology terminology. For the current study, the authors applied this new classification for pancreaticobiliary cytology to a set of previously diagnosed endoscopic ultrasound (EUS)-guided fine-needle aspir...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21559
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Tumor spread through air spaces (STAS), a significant prognostic indicator, has been described recently as a pattern of invasion in pulmonary carcinomas. However, questions remain regarding preoperative identification of STAS and whether it represents an in vivo phenomenon versus an ex vivo artifact. METHOD...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22243
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:The accumulation of cyclin-dependent kinase inhibitor 2A (p16ink4a ) protein in a cell is associated with neoplastic progression in precancerous cervical lesions. Dual staining for p16ink4a and Ki-67 has been proposed as a triage test in cervical cancer screening for women who test positive for human papillo...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21800
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Liquid-based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lun...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22069
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:Mantle cell lymphoma (MCL) demonstrates cytologic features that overlap with those of other types of B-cell non-Hodgkin lymphomas (B-cell NHLs) containing small to medium-sized cells. The accurate diagnosis of MCL is important because MCL has relatively more aggressive biologic behavior and thus requires spe...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21465
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:BRAF mutations are highly specific for papillary thyroid carcinoma (PTC) and many cytology specimens with BRAF mutations are expected to demonstrate cytologic features typical of PTC. However, indeterminate thyroid cytology cases are inevitable and understanding the significance of the BRAF mutation within t...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21229
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Fine-needle aspiration (FNA) is widely accepted in the preoperative management of salivary gland lesions. The proposed Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) aims to standardize reporting terminology. Studies regarding the risk of malignancy (ROM) for the proposed categories continu...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22016
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:New developments in the treatment of lung cancer have necessitated the further histologic and cytologic subtyping of nonsmall cell lung carcinomas. Thyroid transcription factor-1 (TTF-1) long has served as the predominant marker for demonstrating lung origin. However, it is also expressed in a variety of oth...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.20108
更新日期:2010-12-25 00:00:00
abstract:BACKGROUND:The Milan System for Reporting Salivary Gland Cytopathology is a tiered classification scheme that includes 6 diagnostic categories. Neoplasm, which is 1 of the 6 proposed categories, consists of benign neoplasm and neoplasm of uncertain malignant potential (NUMP). NUMP is reserved for a salivary gland neopl...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.22002
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:The objective of this study was to assess how atypical diagnostic category (ADC) is followed up, its outcomes, and the predictors that are associated with subsequent diagnosis of neoplasm/malignancy. METHODS:We reviewed pancreatic endoscopic ultrasound fine-needle aspiration (EUS-FNA) with ADC and compared ...
journal_title:Cancer cytopathology
pub_type: 杂志文章
doi:10.1002/cncy.21389
更新日期:2014-06-01 00:00:00