Abstract:
:About 55% of the acyl groups of dog and human vitreous are unsaturated fatty acids. The major components are oleate (18:1, n-9) and arachidonate (20:4, n-6) with moderate amounts of linoleate (18:2, n-6) and docosahexaenoate (22:6, n-3). Palmitate (16:0) and stearate (18:0) are the major saturated fatty acids. There are no significant changes between ages 37-82 years in the fatty acyl group content and composition of human vitreous. In vitreous from Irish setters with hereditary rod-cone dysplasia (RCD) the levels of oleate are decreased with a concomitant increase in arachidonate. [1-14C]Arachidonic acid was actively incorporated into canine vitreous glycerolipids both in vitro and in vivo. The incorporation was mainly into phosphatidylinositol, triacylglycerol, phosphatidylcholine and phosphatidylethanolamine. There were some differences in the pattern of incorporation between human and dog and between in vivo and in vitro incubations of canine vitreous. Glycerolipid acylation was significantly increased in phosphatidylinositol and phosphatidylcholine in RCD canine vitreous. The pattern of incorporation of [U-14C]docosahexaenoic acid into vitreous glycerolipids was different from arachidonic acid incorporation. Although vitreous did not produce any measurable enzymatic synthesis of cyclooxygenase and lipoxygenase products from [1-14C]-arachidonic acid in vitro, there was significant generation of autooxidation products. These results suggest an active lipid metabolism in vitreous.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Reddy TS,Birkle DL,Packer AJ,Dobard P,Bazan NGdoi
10.3109/02713688609015113subject
Has Abstractpub_date
1986-06-01 00:00:00pages
441-7issue
6eissn
0271-3683issn
1460-2202journal_volume
5pub_type
杂志文章abstract::Corneas and tendons dissected from 17-day-old chick embryos were labeled with [35S]methionine in the presence of 0.3 mM alpha,alpha'-dipyridyl. The unhydroxylated, 35S-labeled pro alpha chains and alpha chains were isolated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The pro alpha and alpha chains...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688508999972
更新日期:1985-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490516198
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journal_title:Current eye research
pub_type: 杂志文章
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journal_title:Current eye research
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更新日期:2008-10-01 00:00:00
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journal_title:Current eye research
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doi:10.1076/ceyr.19.1.1.5345
更新日期:1999-07-01 00:00:00
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doi:10.3109/02713683.2011.599105
更新日期:2011-10-01 00:00:00
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journal_title:Current eye research
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doi:10.1076/ceyr.16.5.503.7049
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journal_title:Current eye research
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pub_type: 杂志文章
doi:10.1076/ceyr.28.1.17.23488
更新日期:2004-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809015147
更新日期:1988-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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更新日期:1998-02-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713683.2011.556300
更新日期:2011-05-01 00:00:00
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doi:10.1076/ceyr.25.2.69.10156
更新日期:2002-08-01 00:00:00
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journal_title:Current eye research
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journal_title:Current eye research
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更新日期:1988-07-01 00:00:00
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更新日期:2020-06-01 00:00:00
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journal_title:Current eye research
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更新日期:1994-07-01 00:00:00
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journal_title:Current eye research
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更新日期:1990-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713689409167301
更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2020-08-30 00:00:00
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更新日期:2020-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209011629
更新日期:1982-01-01 00:00:00