当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > A predominant form of C-terminally end-cleaved AQP0 functions as an open water channel and an adhesion protein in AQP0ΔC/ΔC mouse lens.

A predominant form of C-terminally end-cleaved AQP0 functions as an open water channel and an adhesion protein in AQP0ΔC/ΔC mouse lens.

Abstract：

:The purpose of this investigation was to find out whether C-terminally end-cleaved aquaporin 0 (AQP0), that is present predominantly in the lens mature fiber cells of the WT, functions as a water channel and a cell-to-cell adhesion (CTCA) protein in a knockin (KI) mouse model (AQP0ΔC/ΔC) that does not express intact AQP0. A genetically engineered KI mouse model, AQP0ΔC/ΔC, expressing only end-cleaved AQP0 was developed. This model expresses 1-246 amino acids of AQP0, instead of the full length 1-263 amino acids. Lens transparency of postnatal day 10 (P10) was analyzed qualitatively by dark field imaging. WT, AQP0+/- and AQP0+/ΔC lenses were transparent; AQP0-/- and AQP0ΔC/ΔC mouse lenses displayed loss of transparency. Lens fiber cell membrane vesicles (FCMVs) were prepared from wild type (WT), AQP0 heterozygous (AQP0+/-), AQP0 knockout (AQP0-/-), AQP0+/ΔC and AQP0ΔC/ΔC; water permeability (Pf) was measured using the osmotic shrinking method. CTCA assay was performed using adhesion-deficient L-cells and FCMVs prepared from the abovementioned genotypes. FCMVs of AQP0+/- and AQP0-/- showed a statistically significant reduction (P < 0.001) in Pf and CTCA compared to those of WT. AQP0+/ΔC and AQP0ΔC/ΔC FCMVs exhibited no statistically significant alteration (P > 0.05) in Pf compared to those of WT. However, CTCA of AQP0+/ΔC AQP0ΔC/ΔC FCMVs was significantly higher (P < 0.001) than that of WT FCMVs. Our experiments clearly show that C-terminally end-cleaved AQP0 can function both as a water channel and a CTCA molecule in the lens fiber cell membranes. Also, end-truncation plays an important role in increasing the CTCA between fiber cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kumari SS,Varadaraj K

doi

10.1016/j.bbrc.2019.02.098

subject

Has Abstract

pub_date

2019-04-09 00:00:00

pages

626-630

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(19)30299-2

journal_volume

511

pub_type

杂志文章

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