Molecular fingerprints to identify Candida species.

Abstract:

:A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes, while MLP identifies microsatellite instability. Both methods generate unambiguous and highly reproducible data. Here, we review the results achieved by using these two techniques and also provide a brief overview of a new method based on high-resolution DNA melting (HRM). This method identifies sequence differences by subtle deviations in sample melting profiles in the presence of saturating fluorescent DNA binding dyes.

journal_name

Biomed Res Int

authors

Spampinato C,Leonardi D

doi

10.1155/2013/923742

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

923742

eissn

2314-6133

issn

2314-6141

journal_volume

2013

pub_type

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