Abstract:
:The aim of this study was to develop a vitrification procedure for human ovarian tissue cryopreservation in order to better preserve the ovarian tissue. Large size samples of ovarian tissue retrieved from 15 female-to-male transgender subjects (18-38 years) were vitrified using two solutions (containing propylene glycol, ethylene glycol, and sucrose at different concentrations) in an open system. Light microscopy, transmission electron microscopy, and TUNEL assay were applied to evaluate the efficiency of the vitrification protocol. After vitrification/warming, light microscopy showed oocyte nucleus with slightly thickened chromatin and irregular shape, while granulosa and stromal cells appeared well preserved. Transmission electron microscopy showed oocytes with slightly irregular nuclear shape and finely dispersed chromatin. Clear vacuoles and alterations in cellular organelles were seen in the oocyte cytoplasm. Stromal cells had a moderately dispersed chromatin and homogeneous cytoplasm with slight vacuolization. TUNEL assay revealed the lack of apoptosis induction by vitrification in all ovarian cell types. In conclusion after vitrification/warming the stromal compartment maintained morphological and ultrastructural features similar to fresh tissue, while the oocyte cytoplasm was slightly damaged. Although these data are encouraging, further studies are necessary and essential to optimize vitrification procedure.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Fabbri R,Vicenti R,Macciocca M,Pasquinelli G,Paradisi R,Battaglia C,Martino NA,Venturoli Sdoi
10.1155/2014/673537subject
Has Abstractpub_date
2014-01-01 00:00:00pages
673537eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::D-Ribose is a five-carbon sugar and generally used as an energy source to improve athletic performance and the ability. The culture conditions for maximum D-ribose production performance from cheap raw material corn starch hydrolysate were improved by using one-factor-at-a-time experiments and a three-level Box-Behnke...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/535097
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To estimate localization and the period up to the appearance of small hyperreflective round-shaped droplets using spectral-domain optical coherence tomography (SD-OCT) after pars plana vitrectomy with silicone oil tamponade. METHODS:A retrospective observational study included 24 patients who had undergone par...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/372045
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To assess whether high-molecular-weight proteins excretion predicts outcome and therapy-responsiveness in patients with FSGS and nephrotic syndrome. RESEARCH DESIGN AND METHODS:Thirty-eight patients measured at biopsy fractional excretion of IgG (FEIgG) and urinary α2-macroglobulin/creatinine ratio ( α m/C)....
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/941831
更新日期:2013-01-01 00:00:00
abstract::Angiogenesis and lymphangiogenesis are important in the proliferation and survival of the malignant hematopoietic neoplasms, including non-Hodgkin's lymphomas (NHLs). Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) play an important role in the initiation of angiogenesis. Both VEGF ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/159813
更新日期:2013-01-01 00:00:00
abstract:Background:Pakistan is a country with one of the highest burden of tuberculosis (TB) in the world, and therefore, it is imperative to revisit the design of behaviour change interventions in the program. This study was designed to understand and assess the knowledge, awareness, perceptions, and health-seeking behaviour ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1850541
更新日期:2020-04-21 00:00:00
abstract::Rheumatoid arthritis (RA) is a systemic multifactorial autoimmune disorder. The interactions between diverse environmental and genetic factors lead to the onset of this complex autoimmune disorder. Serum levels of vitamin D (VD) are involved in the regulation of various immune responses. Vitamin D is a key signaling m...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/3494937
更新日期:2019-05-07 00:00:00
abstract:BACKGROUND:Histones play important roles in both host defenses and inflammation related to microbial infection. A peptide mimotope (SSV) was identified from a novel histone H1 monoclonal antibody (16G9 mAb) that was shown to inhibit the mixed lymphocyte reaction. In the present study, an anti-SSV producing hybridoma wa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/491649
更新日期:2015-01-01 00:00:00
abstract::Energy dispersive X-ray spectroscopy microanalysis (EDX), scanning electron microscopy (SEM), and Archimedes' Principle were used to determine the characteristics of inorganic filler particles in five dental alginates, including Cavex ColorChange (C), Hydrogum 5 (H5), Hydrogum (H), Orthoprint (O), and Jeltrate Plus (J...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/178064
更新日期:2014-01-01 00:00:00
abstract::Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/538786
更新日期:2015-01-01 00:00:00
abstract:AIM:To create a predictive score for the discrimination between benign and malignant parotid tumors using elastographic parameters and to compare its sensitivity and specificity with standard ultrasound. METHODS:A total of 124 patients with parotid gland lesions for whom surgery was planned were examined using convent...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9234672
更新日期:2017-01-01 00:00:00
abstract::This research assessed the seasonal variation of the chemical composition and antibacterial and anticholinesterase activities of essential oils extracted from M. longifolia leaves. The leaves organic fractions were also investigated for their biological activities and pharmacological functions. The essential oil highe...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/7856517
更新日期:2018-12-09 00:00:00
abstract::Besides the pinpointing of individual disease-related genes, associating protein complexes to human inherited diseases is also of great importance, because a biological function usually arises from the cooperative behaviour of multiple proteins in a protein complex. Moreover, knowledge about disease-related protein co...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/732650
更新日期:2013-01-01 00:00:00
abstract::In order to understand disease pathogenesis, improve medical diagnosis, or discover effective drug targets, it is important to identify significant genes deeply involved in human disease. For this purpose, many earlier approaches attempted to prioritize candidate genes using gene expression profiles or SNP genotype da...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2015/576349
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To evaluate the effect of applied suction during microkeratome-assisted laser in situ keratomileusis (LASIK) procedure on peripapillary retinal nerve fiber layer (RNFL) thickness as well as macular ganglion cell-inner plexiform layer (GC-IPL) thickness. METHODS:89 patients (124 eyes) with established myopia ra...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/8923819
更新日期:2017-01-01 00:00:00
abstract:Background:Neonatal jaundice is common a clinical problem worldwide. Globally, every year, about 1.1 million babies develop severe hyperbilirubinemia with or without bilirubin encephalopathy and the vast majority reside in sub-Saharan Africa and South Asia. Strategies and information on determinants of neonatal jaundic...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4743974
更新日期:2020-10-21 00:00:00
abstract:Background:Sympathetic sprouting in the dorsal root ganglion (DRG) following nerve injuries had been proved to induce adult neuropathic pain. However, it is unclear whether the abnormal sprouting occurs in infant nerve injury. Methods:L5 spinal nerve ligation (SNL) or sham surgery was performed on adult rats and 10-da...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9165475
更新日期:2020-06-16 00:00:00
abstract::A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/m...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/743812
更新日期:2014-01-01 00:00:00
abstract:Purpose of Review:Transversus abdominis plane (TAP) block is a regional technique for analgesia of the anterolateral abdominal wall. This review highlights the nomenclature system and recent advances in TAP block techniques and proposes directions for future research. Recent Findings:Ultrasound guidance is now conside...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/8284363
更新日期:2017-01-01 00:00:00
abstract::This work aimed to explore the cardiovascular effects induced by freeze-dried juice from Syzygium jambolanum (Lam.) DC fruits (JSJ). JSJ presented high polyphenol content and steroids. HPLC analysis revealed that 2,5-dihydroxybenzoic and caffeic acid were present in higher amounts in the JSJ extract. In rat, JSJ induc...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/4827461
更新日期:2018-10-08 00:00:00
abstract:OBJECTIVE:Greater skeletal muscle fat infiltration occurs with age and contributes to numerous negative health outcomes. The primary purpose was to determine whether intermuscular adipose tissue (IMAT) can be influenced by an exercise intervention and if a greater reduction in IMAT occurs with eccentric versus traditio...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2014/398960
更新日期:2014-01-01 00:00:00
abstract:Background:Anion gap (AG) has been proved to be associated with prognosis of many cardiovascular diseases. This study is aimed at exploring the association of AG with inhospital all-cause mortality and adverse clinical outcomes in coronary care unit (CCU) patients. Method:All data of this study was extracted from Medi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4598462
更新日期:2020-08-27 00:00:00
abstract::This study aimed to analyse the diversity of the Campylobacter jejuni population in broilers and to evaluate the major source of contamination in poultry meat. Eight rearing cycles over one year provided samples from three different broiler farms processed at the same slaughterhouse. A total of 707 C. jejuni were isol...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/859845
更新日期:2015-01-01 00:00:00
abstract::The recombinant catalase-peroxidase HPI from E. coli was used as an alternative enzyme in polymerization reactions for the production of (-) epicatechin oligomers and their biological activity was characterized. The enzyme was prepared in two forms: a purified and an immobilized form. Both were tested for their activi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/3828627
更新日期:2018-04-23 00:00:00
abstract::Neonatal hyperbilirubinemia is a common problem with potentiality to cause irreversible brain damage. Reduction of serum bilirubin level is essential to minimize such damage. Compact fluorescent tubes, halogen bulbs, fiber optic blankets, and LEDs are commonly used light sources for phototherapy with varying efficacie...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2019/6274719
更新日期:2019-04-11 00:00:00
abstract:Background:Musculoskeletal disorders (MSDs) are considered one of the most common health issues in working population and have a high social and economic impact. This study is aimed at determining the MSD patterns and associated risk factors among higher education academicians in Saudi Arabia. Methods:A cross-sectiona...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8930968
更新日期:2020-08-15 00:00:00
abstract::Hepatitis B virus (HBV) is an important public health problem that poses a serious threat to human health. HBV reactivation generally occurs in overt or occult HBV infection patients who suffered DDAs, chemotherapy, or immunosuppressive therapy, especially when some solid tumors and leukemia patients are using hormone...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/2931402
更新日期:2018-12-26 00:00:00
abstract:Objectives:The purpose of the study was to evaluate the correlation between dynamic test results obtained on a stabilometric platform and the results achieved on the Y-balance test (Y-BT). Method:The study group consisted of 52 adolescent athletes, aged 14 to 17 years. Each participant was evaluated in the scope of th...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6968473
更新日期:2020-11-12 00:00:00
abstract::With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compar...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/408683
更新日期:2014-01-01 00:00:00
abstract::Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and a major cause of nonrelapse mortality after allo-HSCT. A conditioning regimen plays a pivotal role in the development of aGVHD. To provide a platform for studying aGVHD and evaluating t...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3071214
更新日期:2016-01-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is the most frequent cause of chronic liver disease. NAFLD encompasses a wide range of pathologies, from simple steatosis to steatosis with inflammation to fibrosis. The pathogenesis of NAFLD progression has not been completely elucidated, and different liver cells could be imp...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/768071
更新日期:2015-01-01 00:00:00