Abstract：
OBJECTIVES:We sought to describe the genetic complexity of 14 UM-SCC oral cavity cancer cell lines that have remained uncharacterized despite being used as model systems for decades. MATERIALS AND METHODS:We performed exome sequencing on 14 oral cavity UM-SCC cell lines and denote the mutational profile of each line. We used a SNP array to profile the multiple copy number variations of each cell line and use immunoblotting to compare alterations to protein expression of commonly amplified genes (EGFR, PIK3CA, etc.). RNA sequencing was performed to characterize the expression of genes with copy number alterations. RESULTS:The cell lines displayed a highly complex network of genetic aberrations that was consistent with alterations identified in the HNSCC TCGA project including PIK3CA amplification, CDKN2A deletion, as well as TP53 and CASP8 mutations, enabling genetic stratification of each cell line in the panel. Copy number FISH and spectral karyotyping analysis demonstrate that cell lines retain chromosomal heterogeneity. CONCLUSIONS:Collectively, we developed an important resource for future oral cavity HNSCC cell line studies and highlight the complexity of genomic aberrations in cell lines.
journal_name
Oral Oncoljournal_title
Oral oncologyauthors
Ludwig ML,Kulkarni A,Birkeland AC,Michmerhuizen NL,Foltin SK,Mann JE,Hoesli RC,Devenport SN,Jewell BM,Shuman AG,Spector ME,Carey TE,Jiang H,Brenner JCdoi
10.1016/j.oraloncology.2018.10.031subject
Has Abstractpub_date
2018-12-01 00:00:00pages
144-151eissn
1368-8375issn
1879-0593pii
S1368-8375(18)30399-3journal_volume
87pub_type
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