当前位置： SCI文献检索 > ORAL ONCOLOGY期刊下所有文献 > Molecular cloning, isolation and characterisation of ERK3 gene from chewing-tobacco induced oral squamous cell carcinoma.

Molecular cloning, isolation and characterisation of ERK3 gene from chewing-tobacco induced oral squamous cell carcinoma.

Abstract：

:The mitogen activated serine/threonine kinases (MAPKs) constitute extracellular signal-regulated protein kinases (ERKs), c-Jun N-terminal kinases (JNKs) and p38 MAPK, with an important role in cell proliferation and transformation. Earlier studies from our laboratory had indicated a role for MAPK pathway in oral cancer. Our current study was aimed at examining the role of a MAPK-ERK3, in chewing-tobacco associated oral squamous cell carcinoma. We constructed a cDNA library from primary oral cancer tissue, cloned and isolated the ERK3 gene. The gene was sequenced and the sequence submitted to GenBank (Accession number AF420474). The oral cancer ERK3 clone demonstrated 100% homology to human ERK3 isolated from fetal skeletal muscle, with four specific nucleotide alterations in the non-coding region of the gene, comprising deletion of 'TTT' between 2701 and 2705 nt; 'G' to 'T' substitution at 188 nt; insertion of 'A' between 121 and 122 nt, and insertion of 'CTTTA' between 3391 and 3392 nt. Southern analysis of EcoRI genomic digests indicated ERK3 specific fragments of 11, 8.6, 6.5 and 3.2 kb sizes. The mRNA transcript analysis defined a single transcript of 4.5 kb. RT-PCR analysis revealed a three- to eight-fold increase in ERK3 expression in a majority (90%) of oral cancer tissues and peripheral blood cells (61.5%) of the patients, whereas absence or low levels of expression was observed in peripheral blood cells of 74% clinically normal healthy individuals with no tobacco habits, and overexpression in PBC from 26% normal individuals. The alterations in the non-coding region of ERK3 gene cloned from oral cancer tissue, may affect stability or regulation of mRNA, resulting in overexpression in the patient samples. The overexpression of the gene in the normal healthy individuals may be indicative of increased risk of developing oral cancers in this group.

journal_name

Oral Oncol

journal_title

Oral oncology

authors

Rai R,Mahale A,Saranath D

doi

10.1016/j.oraloncology.2004.01.010

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

705-12

issue

7

eissn

1368-8375

issn

1879-0593

pii

S1368837504000235

journal_volume

40

pub_type

杂志文章

相关文献

ORAL ONCOLOGY文献大全
  • Use of artificial intelligence in diagnosis of head and neck precancerous and cancerous lesions: A systematic review.

    abstract：:This systematic review analyses and describes the application and diagnostic accuracy of Artificial Intelligence (AI) methods used for detection and grading of potentially malignant (pre-cancerous) and cancerous head and neck lesions using whole slide images (WSI) of human tissue slides. Electronic databases MEDLINE v...

    journal_title：Oral oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.oraloncology.2020.104885

    authors： Mahmood H,Shaban M,Indave BI,Santos-Silva AR,Rajpoot N,Khurram SA

    更新日期：2020-11-01 00:00:00

  • Management principles of head and neck cancers during pregnancy: a review and case series.

    abstract：:Head and neck cancers during pregnancy are rising in incidence. Clinicians need to be aware of the principles of managing these cases. This paper is a review of the literature of head and neck cancers during pregnancy with a short case series of six non-thyroid pregnancy-associated cancers treated by a head and neck o...

    journal_title：Oral oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.oraloncology.2007.02.003

    authors： Atabo A,Bradley PJ

    更新日期：2008-03-01 00:00:00

  • Imbalance between proliferation and apoptosis may be responsible for treatment failure after postoperative radiotherapy in squamous cell carcinoma of the oropharynx.

    abstract：:To assess the prognostic value of apoptosis, proliferation and clinical factors in squamous cell carcinoma of the oropharynx after radical surgery and postoperative radiotherapy (RT). Between 1985 and 1995, a total of 82 patients with 84 tumors were entered onto the study. Forty-two primary tumors (50%) involved the t...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s1368-8375(03)00005-8

    authors： Grabenbauer GG,Suckorada O,Niedobitek G,Rödel F,Iro H,Sauer R,Rödel C,Schultze-Mosgau S,Distel L

    更新日期：2003-07-01 00:00:00

  • Long-term speech and swallowing function after primary resection and sentinel node biopsy for early oral squamous cell carcinoma.

    abstract：OBJECTIVES:Analysis of long-term speech and swallowing function and subjective quality of life (QOL) after primary resection and sentinel node biopsy (SNB) in patients with early stage (cT1/T2) oral squamous cell carcinomas (OSCC). MATERIAL AND METHODS:Eighty-one consecutive patients treated primarily by transoral res...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2018.12.027

    authors： Romer CAE,Broglie Daeppen MA,Mueller M,Huber GF,Guesewell S,Stoeckli SJ

    更新日期：2019-02-01 00:00:00

  • Angiostatin gene therapy inhibits the growth of murine squamous cell carcinoma in vivo.

    abstract：:Tumor growth is an angiogenesis-dependent process and therapeutic strategies aimed at inhibiting angiogenesis are theoretically attractive. Angiostatin has been shown to potently inhibit endothelial proliferation in vitro and tumor growth in vivo. We now show that a shift in the balance of tumor angiogenesis by gene t...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s1368-8375(00)00100-7

    authors： Matsumoto G,Ohmi Y,Shindo J

    更新日期：2001-06-01 00:00:00

  • Patient-oriented toxicity endpoints after head and neck reirradiation with intensity modulated radiation therapy.

    abstract：OBJECTIVES:To characterize specific serious toxicities of reRT with intensity modulated radiation therapy (IMRT) for squamous cell carcinoma of the head and neck (SCCHN) and identify treatment-related predictors of toxicity for patient counseling and decision-making. MATERIALS/METHODS:75 consecutive patients with recu...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2017.08.012

    authors： Margalit DN,Schoenfeld JD,Rawal B,Haddad RI,Catalano PJ,Goguen LA,Chau NG,Rabinowits G,Lorch JH,Annino DJ,Tishler RB

    更新日期：2017-10-01 00:00:00

  • 'Doing as little as possible and as much as necessary' - Oncological efficacy of marginal mandibulectomy in resection of oral cavity cancers.

    abstract：BACKGROUND:In carefully selected oral cavity cancers, marginal mandibulectomy is an adequate procedure which achieves good disease control adhering to the principle of "doing as little as possible and as much as necessary". METHODOLOGY:This was a retrospective study done by reviewing the medical records of all patient...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2019.05.026

    authors： Suresh S,Thomas S,Janardhan D,Patil S,George NA,Varghese BT,Iype EM,Rafi M,George CK

    更新日期：2019-08-01 00:00:00

  • A comparison of mandible preservation method and mandibulotomy approach in oral and oropharyngeal cancer: A meta-analysis.

    abstract：OBJECTIVE:This study aims to compare the prognoses outcomes of mandibular preservation method (MPM) and the mandibulotomy approach (MLA) in oral and oropharyngeal cancer (OOPC) patients. METHOD:We searched PubMed, Web of Science, EMBASE, Chinese BioMedical Literature Database (CBM), Cochrane Library, and clinicaltrial...

    journal_title：Oral oncology

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.oraloncology.2016.11.004

    authors： Pang P,Li RW,Shi JP,Xu ZF,Duan WY,Liu FY,Huang SH,Tan XX,Sun CF

    更新日期：2016-12-01 00:00:00

  • Quality insurance in head and neck cancer multidisciplinary team meetings: A watchful eye on real-life experience.

    abstract：INTRODUCTION:Although Multidisciplinary Team Management (MDT) is integrated in most international head and neck cancer treatment guidelines, its applications and proceedings were rarely described. The present study explores a 6-year real-life experience in a French Comprehensive Cancer Care Center. METHODS:Patients, t...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2019.02.020

    authors： Guy JB,Benna M,Xia Y,Daguenet E,Ben Mrad M,Jmour O,Vial N,Lelonge Y,Fournel P,Prades JM,Vallard A,Magné N

    更新日期：2019-04-01 00:00:00

  • miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1.

    abstract：OBJECTIVES:miR-29b has been implicated in various cancers. However, the role of miR-29b in tongue squamous cell carcinoma (TSCC) remains unclear. This study aimed to investigate the role of miR-29b in TSCC progression. MATERIALS AND METHODS:The expression of miR-29b was analyzed in TSCC tissues and cells. Functional s...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2014.07.010

    authors： Jia LF,Huang YP,Zheng YF,Lyu MY,Wei SB,Meng Z,Gan YH

    更新日期：2014-11-01 00:00:00

  • Correlation of p16 expression and HPV type with survival in oropharyngeal squamous cell cancer.

    abstract：OBJECTIVES:Examine the effect of concordance between p16 overexpression and HR (high risk) HPV DNA status on overall survival in a large series of oropharyngeal squamous cell carcinoma (OPSCC) cases. MATERIALS AND METHODS:A total of 185 patients with primary OPSCC had genomic DNA tested by PCR for the HPV16 E6 and E7 ...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2015.06.014

    authors： Liu SZ,Zandberg DP,Schumaker LM,Papadimitriou JC,Cullen KJ

    更新日期：2015-09-01 00:00:00

  • Obturators versus flaps after maxillary oncological ablation: A systematic review and best evidence synthesis.

    abstract：:Maxillary defects can be resolved by prosthetic obturation, autologous tissue reconstruction, or a combination of both. However, there is still controversy in the selection of the optimal approach. Therefore, the aim of this study was to systematically review evidences comparing the performance of obturators and flaps...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2018.05.019

    authors： Cao Y,Yu C,Liu W,Miao C,Han B,Yang J,Li L,Li C

    更新日期：2018-07-01 00:00:00

  • Expression of cyclin dependent kinase inhibitor p21waf1/cip1 in premalignant and malignant oral lesions: relationship with p53 status.

    abstract：:p21waf1/cip1 protein, an inhibitor of cyclin dependent kinases, is a critical downstream target in the p53-specific pathway of growth control, and can also be induced by p53 independent pathways in relation to terminal differentiation. p21waf1 is also a putative tumour suppressor. Hence, we sought to determine whether...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s1368-8375(98)00021-9

    authors： Agarwal S,Mathur M,Shukla NK,Ralhan R

    更新日期：1998-09-01 00:00:00

  • Multipotency of CD11bhighGr-1+ immature myeloid cells accumulating in oral squamous cell carcinoma-bearing mice.

    abstract：:We demonstrated the accumulation of CD11b(high)Gr-1(+) cells in a murine model of squamous cell carcinoma (SCC). Inoculation of NR-S1K cells derived from oral SCC induced a rapid and clear accumulation of CD11b(high)Gr-1(+) cells in secondary lymphoid organs as well as in peripheral blood. Phenotypic and morphological...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2006.06.009

    authors： Tanaka K,Jinhua P,Omura K,Azuma M

    更新日期：2007-07-01 00:00:00

  • Paraneoplastic pemphigus caused by an epithelioid leiomyosarcoma and associated with fatal respiratory failure.

    abstract：:A patient is described who initially presented with pemphigus vulgaris, limited to the oral cavity, and weight loss. Although the various laboratory studies pointed to the diagnosis of paraneoplastic pemphigus (PNP), the underlying neoplasm was not detected until 6 months later, when the patient developed shortness of...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s1368-8375(00)00022-1

    authors： van der Waal RI,Pas HH,Nousari HC,Schulten EA,Jonkman MF,Nieboer C,Stoof TJ,Starink TM,Anhalt GJ

    更新日期：2000-07-01 00:00:00

  • Mutation of p53 gene codon 63 in saliva as a molecular marker for oral squamous cell carcinomas.

    abstract：:The inactivation of tumor suppressor gene (TSG) is important during multistage carcinogenesis. The p53 TSG is frequently mutated in oral squamous cell carcinomas. These mutations can serve as very specific markers for the presence of tumor cells in a background of normal cells. In this study, 10 oral squamous cell car...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s1368-8375(00)00005-1

    authors： Liao PH,Chang YC,Huang MF,Tai KW,Chou MY

    更新日期：2000-05-01 00:00:00

  • (18)FDG-PET/CT for detecting distant metastases and second primary cancers in patients with head and neck cancer. A meta-analysis.

    abstract：:We performed a meta-analysis to evaluate the role of (18)F-fluorodeoxyglucose positron emission tomography/computed tomography ((18)FDG-PET/CT) in detecting distant metastases and second primary cancers in patients with head and neck cancer (HNC) at staging. Studies about (18)FDG-PET/CT for detecting distant metastase...

    journal_title：Oral oncology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.oraloncology.2011.04.021

    authors： Xu GZ,Guan DJ,He ZY

    更新日期：2011-07-01 00:00:00

  • EB-D fibronectin expression in squamous cell carcinoma of the head and neck.

    abstract：:ED-B fibronectin (ED-B FN), a glycoprotein involved in cell adhesion and migration, is expressed in fetal and neoplastic tissues and absent in their normal counterparts. The aim of this study is to evaluate the expression of this glycoprotein in relation to the histological and clinical data and to determine whether i...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2004.07.003

    authors： Mhawech P,Dulguerov P,Assaly M,Ares C,Allal AS

    更新日期：2005-01-01 00:00:00

  • Heat shock protein induced TCR gammadelta gene rearrangements in patients with oral cancer.

    abstract：:We analyzed the T cell receptor (TCR) gammadelta gene repertoire in peripheral blood and tumor compartment of oral cancer (OC) patients before and after stimulation with heat shock proteins (hsp), which are known ligands for gammadelta T cells. Clonal TCR gamma and delta gene rearrangements in lymphocytes from tumor c...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2004.08.007

    authors： Wadia P,Atre N,Pradhan T,Mistry R,Chiplunkar S

    更新日期：2005-02-01 00:00:00

  • Immunohistochemical study of acinic cell carcinoma of minor salivary gland.

    abstract：:Acinic cell carcinoma (ACC) is a rare salivary gland tumour, making up 4% of all minor salivary gland tumors. Typically, it is composed of acinic cells although transitional and duct-like cells are also identified. In the present study, a panel of antibodies was applied to eight minor salivary gland ACCs. Antibodies t...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/s0964-1955(96)00064-4

    authors： Crivelini MM,de Sousa SO,de Araújo VC

    更新日期：1997-05-01 00:00:00

  • The genomic landscape of UM-SCC oral cavity squamous cell carcinoma cell lines.

    abstract：OBJECTIVES:We sought to describe the genetic complexity of 14 UM-SCC oral cavity cancer cell lines that have remained uncharacterized despite being used as model systems for decades. MATERIALS AND METHODS:We performed exome sequencing on 14 oral cavity UM-SCC cell lines and denote the mutational profile of each line. ...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2018.10.031

    authors： Ludwig ML,Kulkarni A,Birkeland AC,Michmerhuizen NL,Foltin SK,Mann JE,Hoesli RC,Devenport SN,Jewell BM,Shuman AG,Spector ME,Carey TE,Jiang H,Brenner JC

    更新日期：2018-12-01 00:00:00

  • Summary from an international cancer seminar focused on human papillomavirus (HPV)-positive oropharynx cancer, convened by scientists at IARC and NCI.

    abstract：:Cancer of the oropharynx has attracted considerable attention in recent years given: (1) an increasing incidence in selected populations over the past three decades; (2) the discovery of human papillomavirus (HPV) infection as the driver of the increase, as opposed to the traditional risk factors such as tobacco (smok...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2020.104736

    authors： Kreimer AR,Chaturvedi AK,Alemany L,Anantharaman D,Bray F,Carrington M,Doorbar J,D'Souza G,Fakhry C,Ferris RL,Gillison M,Neil Hayes D,Hildesheim A,Huang SH,Kowalski LP,Lang Kuhs KA,Lewis J Jr,Lowy DR,Mehanna H,Ness A

    更新日期：2020-09-01 00:00:00

  • Confocal laser endomicroscopy for non-invasive head and neck cancer imaging: a comprehensive review.

    abstract：:Histological assessment is an essential tool in the diagnosis and guidance of the treatment of various diseases, in particular cancer, of the head and neck. Recent major advances in optical imaging techniques have made it possible to acquire high-resolution in vivo images at the cellular scale. Confocal endomicroscopy...

    journal_title：Oral oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.oraloncology.2014.05.002

    authors： Abbaci M,Breuskin I,Casiraghi O,De Leeuw F,Ferchiou M,Temam S,Laplace-Builhé C

    更新日期：2014-08-01 00:00:00

  • Complementary serum test of antibodies to Epstein-Barr virus nuclear antigen-1 and early antigen: a possible alternative for primary screening of nasopharyngeal carcinoma.

    abstract：:This hospital-based cohort study evaluated the efficacy of three Epstein-Barr virus (EBV) - associated assays for nasopharyngeal carcinoma (NPC) primary screening and monitoring treatment outcome. Five hundred and seventeen consecutive subjects, including 156 NPC patients, 264 healthy volunteers and 97 patients with h...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2007.10.003

    authors： Chang KP,Hsu CL,Chang YL,Tsang NM,Chen CK,Lee TJ,Tsao KC,Huang CG,Chang YS,Yu JS,Hao SP

    更新日期：2008-08-01 00:00:00

  • Criteria to rationalize population screening to control oral cancer.

    abstract：:Screening populations for the early detection of asymptomatic malignancies and potential malignancies are intuitively attractive strategies to control or reduce the burden of oral cancer on society. Subsequent preventive and/or therapeutic measures must, however, be substantiated by prospective randomized controlled t...

    journal_title：Oral oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.oraloncology.2004.04.009

    authors： Lavelle CL,Scully C

    更新日期：2005-01-01 00:00:00

  • Pretreatment 18F-FDG PET/CT texture parameters provide complementary information to Epstein-Barr virus DNA titers in patients with metastatic nasopharyngeal carcinoma.

    abstract：PURPOSE:The aim of the present study was to evaluate whether texture features extracted from 18F-FDG PET/CT images may provide additional prognostic information in patients with metastatic nasopharyngeal carcinoma (NPC). MATERIALS AND METHOD:We retrospectively examined 52 patients with metastatic NPC who underwent ass...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2020.104628

    authors： Lin HC,Chan SC,Cheng NM,Liao CT,Hsu CL,Wang HM,Lin CY,Chang JT,Ng SH,Yang LY,Yen TC

    更新日期：2020-05-01 00:00:00

  • Computed tomography evaluation after induction chemotherapy for T3 laryngeal cancer: Does response correlate with vocal cord mobility?

    abstract：OBJECTIVES:After induction chemotherapy (IC) for laryngeal cancer, Computed Tomography (CT) is used to assess tumor response but lacks rationalized methods for measurement of this response. In T3 laryngeal cancer, remobilization of an initially fixed vocal cord (VC) is a major sign of tumor response. We compared the pe...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2019.01.009

    authors： Garcia GCTE,Gorphe P,Hartl D,Ammari S,Even C,Tao Y,Balleyguier C,Varoquaux A,Bidault F

    更新日期：2019-03-01 00:00:00

  • Impact of notch signaling on the prognosis of patients with head and neck squamous cell carcinoma.

    abstract：OBJECTIVES:The function of NOTCH signaling (oncogenic or oncosuppressive) remains controversial in head and neck squamous cell carcinomas (HNSCC). The purpose of this work is to investigate the role of NOTCH pathway in HNSCC prognosis. METHODS:Immunohistochemical NOTCH1 and HES1 expression was jointly evaluated and co...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2020.105003

    authors： Grilli G,Hermida-Prado F,Álvarez-Fernández M,Allonca E,Álvarez-González M,Astudillo A,Moreno-Bueno G,Cano A,García-Pedrero JM,Rodrigo JP

    更新日期：2020-11-01 00:00:00

  • Clinical features of post-radiation nasopharyngeal necrosis and their outcomes following surgical intervention in nasopharyngeal cancer patients.

    abstract：PURPOSE:To investigate the incidence and clinical features of post-radiation nasopharyngeal necrosis (PRNN), and effectiveness of surgical intervention for its treatment. MATERIALS AND METHODS:Retrospectively, we reviewed 380 NPC patients who underwent high dose radiotherapy (RT) (single RT in 355 and re-RT in 25) fro...

    journal_title：Oral oncology

    pub_type： 杂志文章

    doi：10.1016/j.oraloncology.2021.105180

    authors： Yang K,Ahn YC,Nam H,Hong SD,Oh D,Noh JM

    更新日期：2021-01-23 00:00:00

  • Late stage diagnosis of oral cancer: components and possible solutions.

    abstract：:Stage of disease at the diagnosis of oral cancer is thought to be a significant factor in prognosis and outcome (International Agency for Research on Cancer/World Health Organization, 2014). Unfortunately, we continue to diagnose almost 2/3 of these cancers at advanced stages of disease despite the ongoing research fo...

    journal_title：Oral oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.oraloncology.2014.09.005

    authors： Güneri P,Epstein JB

    更新日期：2014-12-01 00:00:00