Quantitative proteomic analysis of microdissected oral epithelium for cancer biomarker discovery.

Abstract:

:Specific biomarkers are urgently needed for the detection and progression of oral cancer. The objective of this study was to discover cancer biomarkers from oral epithelium through utilizing high throughput quantitative proteomics approaches. Morphologically malignant, epithelial dysplasia, and adjacent normal epithelial tissues were laser capture microdissected (LCM) from 19 patients and used for proteomics analysis. Total proteins from each group were extracted, digested and then labelled with corresponding isobaric tags for relative and absolute quantitation (iTRAQ). Labelled peptides from each sample were combined and analyzed by liquid chromatography-mass spectrometry (LC-MS/MS) for protein identification and quantification. In total, 500 proteins were identified and 425 of them were quantified. When compared with adjacent normal oral epithelium, 17 and 15 proteins were consistently up-regulated or down-regulated in malignant and epithelial dysplasia, respectively. Half of these candidate biomarkers were discovered for oral cancer for the first time. Cornulin was initially confirmed in tissue protein extracts and was further validated in tissue microarray. Its presence in the saliva of oral cancer patients was also explored. Myoglobin and S100A8 were pre-validated by tissue microarray. These data demonstrated that the proteomic biomarkers discovered through this strategy are potential targets for oral cancer detection and salivary diagnostics.

journal_name

Oral Oncol

journal_title

Oral oncology

authors

Xiao H,Langerman A,Zhang Y,Khalid O,Hu S,Cao CX,Lingen MW,Wong DTW

doi

10.1016/j.oraloncology.2015.08.008

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

1011-1019

issue

11

eissn

1368-8375

issn

1879-0593

pii

S1368-8375(15)00298-5

journal_volume

51

pub_type

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