Abstract：
:Human umbilical cord represents a source of multipotent stromal cells of a supreme therapeutic potential. The cells can be isolated from either fresh or cryopreserved umbilical cord tissues. DMSO is a cryoprotectant most commonly used for preservation of umbilical cord tissues; however, cyto- and genotoxicity of this compound is evident and well documented. In the present study we performed successful cryopreservation of the umbilical cord tissue using other cryoprotectants: propylene glycol, ethylene glycol, and glycerol. Of these, 1.5 M ethylene glycol and 20% glycerol turned out to be the best in terms of the preservation of living cells within the frozen tissue, early onset of migration of these cells out of the thawed explants, and overall efficacy of multipotent stromal cell isolation. Cryobanking of tissues can improve availability of multiple cell products for medical purposes and promote the development of personalized medicine.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Arutyunyan IV,Strokova SО,Makarov АV,Mullabaeva SМ,Elchaninov АV,Lokhonina АV,Abramov АА,Fatkhudinov ТKdoi
10.1007/s10517-018-4305-5subject
Has Abstractpub_date
2018-11-01 00:00:00pages
155-162issue
1eissn
0007-4888issn
1573-8221pii
10.1007/s10517-018-4305-5journal_volume
166pub_type
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