Abstract：
:We studied the rate of oxygen consumption by isolated cardiomyocytes from intact rats and animals with experimental myocardial infarction or streptozotocin-induced diabetes mellitus. The measurements were performed in standard incubation medium under various conditions of oxygenation and after addition of arachidonic acid (20 μmol/liter). Under normoxic conditions, arachidonic acid improves respiration of cardiomyocytes from intact animals, but reduces this parameter in cells isolated from animals with pathologies. The intensity of O2 consumption by cardiomyocytes from intact rats and animals with pathologies was shown to decrease during hypoxia. Addition of arachidonic acid aggravated inhibition of respiration for cardiomyocytes from intact rats and specimens with myocardial infarction, but had no effect in diabetes mellitus. The effect of arachidonic acid on oxygen consumption rate is probably mediated by a nonspecific mechanism realized at the mitochondrial level.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Egorova MV,Kutsykova TV,Afanas'ev SA,Popov SVdoi
10.1007/s10517-015-3124-1subject
Has Abstractpub_date
2015-12-01 00:00:00pages
190-2issue
2eissn
0007-4888issn
1573-8221pii
10.1007/s10517-015-3124-1journal_volume
160pub_type
杂志文章abstract：:We compared cytotoxic activity of blood mononuclear leukocytes from healthy donors and lymphokine-activated killer cells generated from them towards tumor and normal cells. Lymphokine-activated killer cells exhibited higher (in comparison with blood mononuclear leukocytes) killer activity towards tumor cells. Lymphoki...
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