Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries.

Abstract:

:We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.

journal_name

Bull Exp Biol Med

authors

Voronova АD,Stepanova OV,Valikhov MP,Chadin AV,Dvornikov АS,Reshetov IV,Chekhonin VP

doi

10.1007/s10517-018-4025-x

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

523-527

issue

4

eissn

0007-4888

issn

1573-8221

pii

10.1007/s10517-018-4025-x

journal_volume

164

pub_type

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