Abstract:
:We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Voronova АD,Stepanova OV,Valikhov MP,Chadin AV,Dvornikov АS,Reshetov IV,Chekhonin VPdoi
10.1007/s10517-018-4025-xsubject
Has Abstractpub_date
2018-03-01 00:00:00pages
523-527issue
4eissn
0007-4888issn
1573-8221pii
10.1007/s10517-018-4025-xjournal_volume
164pub_type
杂志文章abstract::In rat sinoatrial node, NAD(+) (10 μM) reduced the rate of spontaneous action potentials, duration of action potentials, and the velocity of slow diastolic depolarization, but the rate of action potential front propagation increases. In passed rabbit Purkinje fibers, NAD(+) (10 μM) reduced the duration of action poten...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-015-2919-4
更新日期:2015-06-01 00:00:00
abstract::We studied the effect of a biomedical cell product (bone marrow multipotent mesenchymal stromal cells and products secreted by these cells in conditioned medium) on subpopulation composition of lymphoid cells of central and peripheral lymphoid organs of Wistar rats under normal conditions. Changes in the subpopulation...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-016-3366-6
更新日期:2016-05-01 00:00:00
abstract::Experiments on the model of mouse BCG-induced granulomatous showed that the content of glycosaminoglycans and proteoglycans in the extracellular matrix of the liver and lungs are changed at the early stages of inflammation (days 3 and 30 postinfection) before cell destruction in the organs begins. This is related to d...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-015-2752-9
更新日期:2015-01-01 00:00:00
abstract::The effect of laser radiation on generation of reactive oxygen species in the whole blood from patients with chronic obstructive pulmonary disease was studied by in vitro recording of luminol-dependent chemiluminescence. Laser irradiation of the blood from patients with increased production of reactive oxygen species ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-007-0299-0
更新日期:2007-08-01 00:00:00
abstract::The rats were divided into groups demonstrating extremely high and low stress reactivity depending on the results of testing for the nociceptive threshold and thermolability in response to bacterial lipopolysaccharide administration. Specific structural features of the nucleus and mitochondria were revealed in neurons...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-007-0419-x
更新日期:2007-11-01 00:00:00
abstract::The resistance of native and circulating modified low-density lipoproteins from human blood to spontaneous and polyethylene glycol-induced association was studied by recording light transmission fluctuations. Circulating modified low-density lipoproteins were less resistant to association than native low-density lipop...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-005-0047-2
更新日期:2004-10-01 00:00:00
abstract::We studied changes in the blood cytokine profile of rats 3 h, 1 day, and 8 days after acute stress on the model of 24-h immobilization followed by LPS administration (100 μg/kg intraperitoneally). The concentration of proinflammatory cytokines (particularly of IL-1β and TNFα) significantly decreased at the early stage...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-020-04786-w
更新日期:2020-04-01 00:00:00
abstract::Patch-clamp and concentration jump studies showed that gadolinium ions dose-dependently reduced the amplitude of proton-activated currents (pH 6.5) in isolated Purkinje cells (gadolinium concentration producing a half-maximum effect was 69 microM for the peak and 8 microM for the stationary component of the current). ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-008-0077-7
更新日期:2008-03-01 00:00:00
abstract::Pro-Gly-Pro and its metabolite Gly-Pro effectively prevented the development of erosive and ulcerative lesions of the gastric mucosa in rats under conditions of water-immersion restraint stress by restoring the oxidatant-antioxidant balance in the total fraction of gastric mucosa cells. Pro-Gly was least effective in ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-010-0866-7
更新日期:2010-07-01 00:00:00
abstract::Low-dose (250 mg daily) oral probucol produces a significant antioxidant effect in coronary patients: increases activity of glutathione peroxidase (enzyme utilizing lipoperoxides) and reduces the content of free-radical oxidation products in the blood. Probucol therapy for 7 days before and for 6 months after coronary...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10517-007-0399-x
更新日期:2007-11-01 00:00:00
abstract::Immunization of mice with synthetic peptide fragments of conservative sites of meningococcal outer membrane proteins led to defense formation against infection with virulent serogroup A and B Meningococci. The role of cellular immunity in the formation of defense against meningococcal infection after immunization with...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-007-0223-7
更新日期:2007-06-01 00:00:00
abstract::We proposed new method for measuring dynamic changes in hunger motivation in rabbits in the course of satisfaction of nutritional need by weight, time, and rate parameters of effective food-procuring behavior. Transformation of the amount of food eaten into the electric signals was performed using electronic weighing ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-012-1629-4
更新日期:2012-05-01 00:00:00
abstract::Cardiac and plasma activities of marker lysosomal enzymes were studied in Wistar rats with metabolic (epinephrine) and occlusion (ligation of coronary arteries) myocardial infarction. Activity of all examined lysosomal enzymes significantly increased in the myocardium and blood plasma starting from the first day after...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/BF02434867
更新日期:2000-06-01 00:00:00
abstract::We studied high-resolution (1)H nuclear magnetic resonance spectra of the serum and serum albumin from patients with the first episode of schizophrenia and healthy individuals. A relative increase in signal intensities of CH(2) protons in serum LDL and VLDL in schizophrenia was demonstrated. Higher intensities of CH(2...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-012-1622-y
更新日期:2012-04-01 00:00:00
abstract::We studied the specific features of ultrasonic vocalization of Wistar rats in various motivational and emotional states. No significant changes were found in ultrasonic vocalization of rats during experimental food motivation and after the satisfaction of food requirements. The state of thirst and satisfaction of wate...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-012-1831-4
更新日期:2012-10-01 00:00:00
abstract::Ten-minute perfusion of intact isolated rat heart with Krebs-Henseleit solution containing delta-opioid receptor agonists (DPDPE, (-)-TAN-67) or delta-opioid receptor antagonists (naltrindole, TIPP[psi], ICI 174,864) at a final concentration of 0.1 mg/liter decreased HR, blood pressure in the left ventricle, and the r...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-006-0188-y
更新日期:2006-04-01 00:00:00
abstract::Local application of ointment with Bacillus spp. strain MG8 (15,000-20,000 living bacterial cells), isolated from permafrost specimens, on the skin wound of about 60 mm(2) stimulated the reparation processes in experimental mice. A possible mechanism stimulating the regeneration of the damaged tissues under the effect...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-015-2789-9
更新日期:2015-02-01 00:00:00
abstract::The possibility of using hyaluronidase for the regulation of the state of different pools of mesenchymal precursors in vivo was demonstrated. The reaction of mesenchymal precursor cells depended on the dose of the enzyme. Administration of hyaluronidase in a dose of 20 U/mouse increased the content of stromal precurso...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-007-0176-x
更新日期:2007-04-01 00:00:00
abstract::We analyzed association of potentially regulatory polymorphisms (rs590352, rs11542583, rs3829202, rs207258, and rs4796672) with breast cancer. A significant association was found between this disease and rs2072580T>A (p=0.001) located in the overlapping promoter regions of the SART3 and ISCU genes. In women with AA an...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-020-04829-2
更新日期:2020-05-01 00:00:00
abstract::We studied carbohydrate specificity and isotypes of antibodies to BSA-conjugated tetrasaccharide, a repeating unit of the capsular polysaccharide of Streptococcus pneumoniae serotype 14, in mouse polyclonal sera and hybridoma-synthesized products. Natural IgM antibodies to the tetrasaccharide containing epitopes simil...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-019-04376-5
更新日期:2019-02-01 00:00:00
abstract::The effects of intranasal pretreatment with antibodies against glutamate on focal penicillin-induced epileptic activity were studied by recording electrocorticogram in non-anesthetized freely moving male Wistar rats. Anticonvulsant effects of intranasal administration of anti-glutamate antibodies (300 μg/kg) 1 h befor...
journal_title:Bulletin of experimental biology and medicine
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doi:10.1007/s10517-013-2206-1
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abstract::Morphological and histoenzymatic changes in cells of the spinal cord and spinal ganglia after epidural injection of a combination of prosidol with clonidine were studied on dogs. No pathological structural and metabolic changes in the nervous tissue were found after combined treatment with the test drugs. Higher activ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1023/a:1013195407388
更新日期:2001-09-01 00:00:00
abstract::We studied the dynamics of indexes for the system of endogenous regulation of NO bioavailability. The content of NO synthase inhibitors (monomethylarginine and asymmetric dimethylarginine) in the blood of mice was measured after intraperitoneal injections of a nonionic surfactant poloxamer 407 for 2 and 14 weeks. The ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-015-3086-3
更新日期:2015-11-01 00:00:00
abstract::Intensive exercise triggers the cascade processes of body adaptation, including modulation of splisosome functioning, and can lead to modification of its activity and choice of alternative exons. We studied the effect of exercise of the maximum aerobic power on activation of transcription of genes involved in the spli...
journal_title:Bulletin of experimental biology and medicine
pub_type: 临床试验,杂志文章
doi:10.1007/s10517-009-0593-0
更新日期:2009-06-01 00:00:00
abstract::Studies of the effects of Trichoderma harzianum Rifai F-180 culture fluid concentrate containing L-lysine-α-oxidase antitumor enzyme produced by the fungus and the homogenous enzyme, on ultrahazardous bacterium Acidovorax citrulli demonstrated the antibacterial activity of the concentrate. Trichoderma harzianum Rifai ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-018-4012-2
更新日期:2018-03-01 00:00:00
abstract::Experiments on rats showed that blockade of hyperpolarization-activated currents moderates tachycardia induced by beta-adrenoceptor agonist isoproterenol and potentiates the increase in stroke volume produced by this agonist. Electrical stimulation of the vagus nerve against the background of isoproterenol treatment a...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1023/a:1019884931598
更新日期:2002-05-01 00:00:00
abstract::In biopsy specimens of the gastric and duodenal mucosa from patients with psoriasis we revealed severe degenerative and dystrophic changes in cells of the surface and glandular epithelium with destruction of functionally important cytoplasmic organelles. The stroma of the gastric mucosa was characterized by hyperplasi...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1023/a:1022658818267
更新日期:2002-11-01 00:00:00
abstract::Ready dosage form (eye drops) prepared on the basis of recombinant alpha2-IFN exhibits high activity towards herpes simplex type 1 virus in vitro. Systematic study of the antiherpesvirus effect of this drug in combination with modified nucleosides showed an inhibitory effect of the synergic type. Combination of IFN pr...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-006-0263-4
更新日期:2006-06-01 00:00:00
abstract::The effects of photostimulation with different colors on α-rhythm amplitude and parameters of variation pulsometry were studied. Green color stimulation modulates human functions: ?-rhythm amplitude increases by 1.06 μV/√Hz (p<0.05) and the index of regulatory systems strain decreases significantly (p<0.05). ...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1007/s10517-010-1046-5
更新日期:2010-11-01 00:00:00
abstract::In August rats more resistant to acute stress-induced gastric damage than Wistar rats, preadaptation to nondamaging stress exposure did not prevent damage and even potentiated these damages. By contrast, in Wistar rats such adaptation decreased gastric damage caused by acute stress. Higher initial resistance of August...
journal_title:Bulletin of experimental biology and medicine
pub_type: 杂志文章
doi:10.1023/a:1021987626693
更新日期:2002-10-01 00:00:00