Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone.

abstract：:Analysis of complex formation between amyloid-β fragments using surface plasmon resonance biosensing and electrospray mass spectrometry reveals that region 11-14 mediates zinc-induced dimerization of amyloid-β and may serve as a potential drug target for preventing development and progression of Alzheimer's disease. ...

journal_title：Molecular bioSystems

authors： Kozin SA,Mezentsev YV,Kulikova AA,Indeykina MI,Golovin AV,Ivanov AS,Tsvetkov PO,Makarov AA

更新日期：2011-04-01 00:00:00

abstract：:The study of mitochondria and mitochondrial Ca2+ signalling in localised regions is hampered by the lack of tools that can uncouple the mitochondrial membrane potential (DeltaPsi(m)) in a spatially predefined manner. Although there are a number of existing mitochondrial uncouplers, these compounds are necessarily memb...

journal_title：Molecular bioSystems

authors： Avlonitis N,Chalmers S,McDougall C,Stanton-Humphreys MN,Brown CT,McCarron JG,Conway SJ

更新日期：2009-05-01 00:00:00

abstract：:Insight into the molecular mechanism of complex diseases is an important topic in the current bio-medical research. However, different from the single-gene disorders, high heterogeneity of many of the complex diseases prevents scientists from the exact understanding of the etiology. In this study, we used Myelodysplas...

journal_title：Molecular bioSystems

authors： Ren X,Zhou X,Chang CC

更新日期：2011-06-01 00:00:00

abstract：:Structural insight into DNA quadruplex structures formed by oligodeoxyribonucleotides 3'TG5'-5'GGGT3' (QS55) and 5'TG3'-3'GGGT5' (QS33) is presented. NMR analysis reveals that QS33 forms a parallel-like four-fold symmetric quadruplex, while QS55 possesses a two-fold symmetry and is characterized by a tetrameric antipa...

journal_title：Molecular bioSystems

authors： Galeone A,Mayol L,Virgilio A,Virno A,Randazzo A

更新日期：2008-05-01 00:00:00

abstract：:Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...

journal_title：Molecular bioSystems

authors： Lu B,Sun YX,Li YQ,Zhang XZ,Zhuo RX

更新日期：2009-06-01 00:00:00

abstract：:The ectonucleotide phosphodiesterase/pyrophosphatase-1 (NPP1) is a type II transmembrane glycoprotein that regulates extracellular inorganic purine nucleotide and inorganic diphosphate levels through the hydrolysis of ATP into AMP and diphosphate. NPP1 is a promising drug target as it plays a role in several disorders...

journal_title：Molecular bioSystems

authors： Barbeau X,Mathieu P,Paquin JF,Lagüe P

更新日期：2017-05-30 00:00:00

abstract：:Non-ribosomal peptide synthetases (NRPSs) are enzymes that catalyze ribosome-independent production of small peptides, most of which are bioactive. NRPSs act as peptide assembly lines where individual, often interconnected modules each incorporate a specific amino acid into the nascent chain. The modules themselves co...

journal_title：Molecular bioSystems

authors： Beer R,Herbst K,Ignatiadis N,Kats I,Adlung L,Meyer H,Niopek D,Christiansen T,Georgi F,Kurzawa N,Meichsner J,Rabe S,Riedel A,Sachs J,Schessner J,Schmidt F,Walch P,Niopek K,Heinemann T,Eils R,Di Ventura B

更新日期：2014-07-01 00:00:00

abstract：:Protein tyrosine phosphatases (PTPs) constitute a large family of enzymes that play key roles in cell signaling. Deregulation of PTP activity results in aberrant tyrosine phosphorylation, which has been linked to the etiology of several human diseases, including cancer. Since phosphate removal by the PTPs can both enh...

journal_title：Molecular bioSystems

authors： Liang F,Kumar S,Zhang ZY

更新日期：2007-05-01 00:00:00

abstract：:Secretagogin is a hexa EF-hand Ca(2+)-binding protein expressed in neuroendocrine, pancreatic endocrine and retinal cells. The protein has been noted for its expression in specific neuronal subtypes in the support of hierarchical organizing principles in the mammalian brain. Secretagogin has previously been found to i...

journal_title：Molecular bioSystems

authors： Bauer MC,O'Connell DJ,Maj M,Wagner L,Cahill DJ,Linse S

更新日期：2011-07-01 00:00:00

abstract：:Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...

journal_title：Molecular bioSystems

authors： Kumar KM,Anbarasu A,Ramaiah S

更新日期：2014-04-01 00:00:00

abstract：:Are protein functions continuous or discretized? Proteomics investigations are starting to address this non-trivial awesome question focusing upon determining the nature of biological molecular relationships. In the following editorial we present a number of experimental studies published in this themed Proteomics Iss...

journal_title：Molecular bioSystems

authors： Urbani A,Castagnola M,Fasano M,Bini L,Modesti A,Timperio AM,Roncada P

更新日期：2013-06-01 00:00:00

abstract：:Infectious diseases caused by bacterial pathogens pose a major concern to public health and, thus, greater attention must be given to providing insightful knowledge on host-pathogen interactions. There are several theories addressing the dynamics of complex mechanisms of host-pathogen interactions. The availability of...

journal_title：Molecular bioSystems

authors： Kamaladevi A,Marudhupandiyan S,Balamurugan K

更新日期：2017-11-21 00:00:00

abstract：:Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO˙, ˙O(2)(-) and ˙OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO˙, HPLC-MS aided PC12 cell systems were developed, and the conversions of 2-...

journal_title：Molecular bioSystems

authors： Wang Y,Jiang X,Liu J,Zhao M,Kang G,Wu J,Peng L,Peng S

更新日期：2011-05-01 00:00:00

abstract：:Human mitogen-activated protein kinase (MAPK) family members JNK and p38 are two homologous protein-serine/threonine kinases but play distinct roles in the pathological process of neurological disorders. Selective targeting of JNK over p38 has been established as a potential therapeutic approach to epilepsy and other ...

journal_title：Molecular bioSystems

authors： Zhuo ZH,Sun YZ,Jin PN,Li FY,Zhang YL,Wang HL

更新日期：2016-07-19 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is one of the most commonly occurring metabolic and endocrinological disorders affecting women of reproductive age. Metabolomics is an emerging field that holds promise in understanding disease pathophysiology. Recently, a few metabolomics based studies have been attempted in PCOS pati...

journal_title：Molecular bioSystems

authors： RoyChoudhury S,Mishra BP,Khan T,Chattopadhayay R,Lodh I,Datta Ray C,Bose G,Sarkar HS,Srivastava S,Joshi MV,Chakravarty B,Chaudhury K

更新日期：2016-10-18 00:00:00

abstract：:It is now widely accepted that microRNAs (miRNAs or miRs) along with transcription factors (TFs) weave a complex inter-regulatory network within the cell that is responsible for the combinatorial regulation of gene expression. Recently we have shown that miRNAs and TFs that form network clusters are also associated wi...

journal_title：Molecular bioSystems

authors： Sengupta D,Bandyopadhyay S

更新日期：2013-06-01 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:In order to elucidate the effect of flexible linker length on the catalytic efficiency of fusion proteins, two short flexible peptide linkers of various lengths were fused between Arabidopsis thaliana 4-coumaroyl-CoA ligase (4CL) and Polygonum cuspidatum stilbene synthase (STS) to generate fusion proteins 4CL-(GSG)n-S...

journal_title：Molecular bioSystems

authors： Guo H,Yang Y,Xue F,Zhang H,Huang T,Liu W,Liu H,Zhang F,Yang M,Liu C,Lu H,Zhang Y,Ma L

更新日期：2017-02-28 00:00:00

abstract：:The biosynthesis of the endocannabinoid anandamide (AEA) and related N-acyl ethanolamine (NAE) lipids is complex and appears to involve multiple pathways, including: (1) direct release of NAEs from N-acyl phosphatidyl ethanolamine (NAPE) precursors by the phosphodiesterase NAPE-PLD, and (2) double O-deacylation of NAP...

journal_title：Molecular bioSystems

authors： Simon GM,Cravatt BF

更新日期：2010-08-01 00:00:00

abstract：:Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...

journal_title：Molecular bioSystems

authors： Pan X,Xiong K

更新日期：2015-08-01 00:00:00

abstract：:The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel. ...

journal_title：Molecular bioSystems

authors： Kim J,Kwon D,Lee J,Pasquier H,Grailhe R

更新日期：2009-02-01 00:00:00

abstract：:To understand the regulatory dynamics of transcription factors (TFs) and their interplay with other cellular components we have integrated transcriptional, protein-protein and the allosteric or equivalent interactions which mediate the physiological activity of TFs in Escherichia coli. To study this integrated network...

journal_title：Molecular bioSystems

authors： Antiqueira L,Janga SC,Costa Lda F

更新日期：2012-11-01 00:00:00

abstract：:The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...

journal_title：Molecular bioSystems

authors： Besold AN,Amick DL,Michel SL

更新日期：2014-07-01 00:00:00

abstract：:Drug promiscuity is one of the key issues in current drug development. Many famous drugs have turned out to behave unexpectedly due to their propensity to bind to multiple targets. One of the primary reasons for this promiscuity is that drugs bind to multiple distinctive target environments, a feature that we call mul...

journal_title：Molecular bioSystems

authors： Park K,Lee S,Ahn HS,Kim D

更新日期：2009-08-01 00:00:00

abstract：:We employed high-temperature classical molecular dynamics (MD) simulations to investigate the unfolding process of the wild-type (WT) and F508del-NBD1 domains of CFTR protein, with and without second-site mutations. To rationalize the in vitro behavior of F508del-NBD1, namely its lower folding yield and higher aggrega...

journal_title：Molecular bioSystems

authors： Estácio SG,Martiniano HF,Faísca PF

更新日期：2016-08-16 00:00:00

abstract：:In the field of synthetic biology, recent genetic engineering efforts have enabled the construction of novel genetic circuits with diverse functionalities and unique activation mechanisms. Because of these advances, artificial genetic networks are becoming increasingly complex, and are demonstrating more robust behavi...

journal_title：Molecular bioSystems

authors： Sayut DJ,Kambam PK,Sun L

更新日期：2007-12-01 00:00:00

abstract：:To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...

journal_title：Molecular bioSystems

authors： Nikolov M,Fischle W

更新日期：2013-02-02 00:00:00

abstract：:Bacterial biosynthesis of lysine has come under increased scrutiny as a target for novel antibacterial agents as it provides lysine for protein synthesis and both lysine and meso-diaminopimelate for construction of the bacterial peptidoglycan cell wall. In this Highlight article we review recent advances in the valida...

journal_title：Molecular bioSystems

authors： Hutton CA,Perugini MA,Gerrard JA

更新日期：2007-07-01 00:00:00

abstract：:Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...

journal_title：Molecular bioSystems

authors： Wehenkel M,Hong JT,Kim KB

更新日期：2008-04-01 00:00:00

abstract：:Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur, which compromise the experimental results. In...

journal_title：Molecular bioSystems

authors： Suttapitugsakul S,Xiao H,Smeekens J,Wu R

更新日期：2017-11-21 00:00:00