HPLC-MS aided PC12 cell systems: to quantitatively monitor the conversion of nitronyl nitroxide in biological systems with and without NO.

abstract：:Protein tyrosine phosphatases are crucial regulators of signal transduction and function as antagonists towards protein tyrosine kinases to control reversible tyrosine phosphorylation, thereby regulating fundamental physiological processes. Growing evidence has supported the notion that reversible oxidative inactivati...

journal_title：Molecular bioSystems

authors： Garcia FJ,Carroll KS

更新日期：2016-05-24 00:00:00

abstract：:Calcium homeostasis is a key factor in the regulation of cardiac excitation-contraction coupling. Calcium dynamics in cardiomyocytes is governed by ATP which depends on insulin dependent glucose concentration, via the glucose transporter type 4 (GLUT4) transporter. It would therefore be interesting to see how calcium ...

journal_title：Molecular bioSystems

authors： Das PN,Kumar A,Bairagi N,Chatterjee S

更新日期：2017-09-26 00:00:00

abstract：:Fabry disease (FD) is an X-linked lysosomal storage disorder caused by a deficiency of the lysosomal hydrolase α-galactosidase A (α-GalA) that leads to the intra-lysosomal accumulation of globotriaosylceramide (Gb3) in various organ systems. As a consequence, a multisystems disorder develops, culminating in stroke, pr...

journal_title：Molecular bioSystems

authors： Matafora V,Cuccurullo M,Beneduci A,Petrazzuolo O,Simeone A,Anastasio P,Mignani R,Feriozzi S,Pisani A,Comotti C,Bachi A,Capasso G

更新日期：2015-06-01 00:00:00

abstract：:Infectious diseases caused by bacterial pathogens pose a major concern to public health and, thus, greater attention must be given to providing insightful knowledge on host-pathogen interactions. There are several theories addressing the dynamics of complex mechanisms of host-pathogen interactions. The availability of...

journal_title：Molecular bioSystems

authors： Kamaladevi A,Marudhupandiyan S,Balamurugan K

更新日期：2017-11-21 00:00:00

abstract：:Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather th...

journal_title：Molecular bioSystems

authors： Altman S

更新日期：2007-09-01 00:00:00

abstract：:A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells. ...

journal_title：Molecular bioSystems

authors： Moreau L,Barthélémy P,Li Y,Luo D,Prata CA,Grinstaff MW

更新日期：2005-09-01 00:00:00

abstract：:The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...

journal_title：Molecular bioSystems

authors： Besold AN,Amick DL,Michel SL

更新日期：2014-07-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are key signaling proteins that regulate how cells interact with their environment. Traditional signaling cascades involving GPCRs have been well described and are well established and very important clinical targets. With the development of more recent technologies, hints about the...

journal_title：Molecular bioSystems

authors： Zhang X,Eggert US

更新日期：2013-04-05 00:00:00

abstract：:TGF-β1, 2 and 3 cytokines are involved in many cellular processes including cell proliferation, differentiation, migration and survival. Whereas TGF-β2 and 3 play important roles in embryonic development, TGF-β1 is mostly implicated in controlling immune responses after birth. The production of TGF-β1 is a tightly reg...

journal_title：Molecular bioSystems

authors： Stockis J,Dedobbeleer O,Lucas S

更新日期：2017-09-26 00:00:00

abstract：:Conventional methods for detecting single nucleotide polymorphisms (SNPs), including direct DNA sequencing, pyrosequencing, and melting curve analysis, are to a great extent limited by their requirement for particular detection instruments. To overcome this limitation, we established a novel SNP detection technique ut...

journal_title：Molecular bioSystems

authors： Imai K,Ogai Y,Nishizawa D,Kasai S,Ikeda K,Koga H

更新日期：2007-08-01 00:00:00

abstract：:The induction of fungal metabolites by fungal co-cultures grown on solid media was explored using multi-well co-cultures in 2 cm diameter Petri dishes. Fungi were grown in 12-well plates to easily and rapidly obtain the large number of replicates necessary for employing metabolomic approaches. Fungal culture using suc...

journal_title：Molecular bioSystems

authors： Bertrand S,Azzollini A,Schumpp O,Bohni N,Schrenzel J,Monod M,Gindro K,Wolfender JL

更新日期：2014-07-29 00:00:00

abstract：:Rule-based modeling provides a means to represent cell signaling systems in a way that captures site-specific details of molecular interactions. For rule-based models to be more widely understood and (re)used, conventions for model visualization and annotation are needed. We have developed the concepts of an extended ...

journal_title：Molecular bioSystems

authors： Chylek LA,Hu B,Blinov ML,Emonet T,Faeder JR,Goldstein B,Gutenkunst RN,Haugh JM,Lipniacki T,Posner RG,Yang J,Hlavacek WS

更新日期：2011-10-01 00:00:00

abstract：:Naphthalene, a polycyclic aromatic hydrocarbon, is a ubiquitous environmental pollutant capable of causing illness. In this study, we deconvoluted the metabolites related to naphthalene intervention in various organs by using nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS...

journal_title：Molecular bioSystems

authors： Ling YS,Liang HJ,Chung MH,Lin MH,Lin CY

更新日期：2014-07-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:Are protein functions continuous or discretized? Proteomics investigations are starting to address this non-trivial awesome question focusing upon determining the nature of biological molecular relationships. In the following editorial we present a number of experimental studies published in this themed Proteomics Iss...

journal_title：Molecular bioSystems

authors： Urbani A,Castagnola M,Fasano M,Bini L,Modesti A,Timperio AM,Roncada P

更新日期：2013-06-01 00:00:00

abstract：:Upon entering biological systems, such as the blood stream, nanoparticles form molecular complexes with the proteins encountered called protein coronas, which shield the surface of the exogenous nanoparticle. The most abundant blood proteins, such as albumin, initially occupy the surface of the nanoparticle. Owing to ...

journal_title：Molecular bioSystems

authors： Ramezani F,Rafii-Tabar H

更新日期：2015-02-01 00:00:00

abstract：:Oxygen dependent modulation of red blood cell metabolism is a long investigated issue. However, the recent introduction of novel mass spectrometry-based approaches lends itself to implement our understanding of the effects of red blood cell prolonged exposure to anaerobiosis. Indeed, most of the studies conducted so f...

journal_title：Molecular bioSystems

authors： D'Alessandro A,Gevi F,Zolla L

更新日期：2013-06-01 00:00:00

abstract：:Epigenetic regulation by histone methylation is crucial for proper programming of the genome during development. Homeostasis of histone methylation is balanced by the activities of histone methyltransferases and demethylases. Although these methyltransferases and demethylases represent logical targets for potential dr...

journal_title：Molecular bioSystems

authors： Liu Y,Platchek M,Kement B,Bee WT,Truong M,Zeng X,Hung S,Lin H,Morrow D,Kallal LA,Xie Q,Agarwal P,Pope AJ,Wu Z

更新日期：2014-02-01 00:00:00

abstract：:Lung fibrosis is a progressive and often fatal lung disease characterized by fibroblast proliferation and excessive deposition of extracellular matrix in the lungs. The chemokine receptor CXCR7 has been shown to control cell adhesion, migration and proliferation by regulating the epithelial-to-mesenchymal transition (...

journal_title：Molecular bioSystems

authors： Guan S,Zhou J

更新日期：2017-09-26 00:00:00

abstract：:The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potent...

journal_title：Molecular bioSystems

authors： Tian J,Ma K,Saaem I

更新日期：2009-07-01 00:00:00

abstract：:The mono-site mutations of the absolutely conserved residues, (464)LGR(466), in the α-helix 5 (h5) of HPV16 L1 completely disrupted the pentamer formation. The implication of this finding is the potential usage of a h5-like peptide as the reagent to interfere with the pentamer formation and stability as an anti-HPV re...

journal_title：Molecular bioSystems

authors： Jin S,Pan D,Zha X,Yu X,Wu Y,Liu Y,Yin F,Chen XS

更新日期：2014-04-01 00:00:00

abstract：:Predicting protein subcellular localization is a challenging problem, particularly when query proteins have multi-label features meaning that they may simultaneously exist at, or move between, two or more different subcellular location sites. Most of the existing methods can only be used to deal with the single-label ...

journal_title：Molecular bioSystems

authors： Lin WZ,Fang JA,Xiao X,Chou KC

更新日期：2013-04-05 00:00:00

abstract：:To identify an agent with specific activity against human lens epithelial cells (HLECs), we confirmed the presence of glucocorticoid receptors (GRs) and GR-α genes and evaluated whether GRs have a relationship with the apoptotic process in cultured HLECs. We also determined whether the inhibitor RU486 could rescue the...

journal_title：Molecular bioSystems

authors： Wang L,Zhao W,Leng F,Ge J,Bu Z,Zhang Y,Liu P

更新日期：2011-06-01 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...

journal_title：Molecular bioSystems

authors： Pirro V,Guffey SC,Sepúlveda MS,Mahapatra CT,Ferreira CR,Jarmusch AK,Cooks RG

更新日期：2016-06-01 00:00:00

abstract：:Protein-protein interactions are important biological processes and essential for a global understanding of cell functions. To date, little is known about the protein interactions and roles of the protein interacting networks and protein complexes in bacterial resistance to antibiotics. In the present study, we invest...

journal_title：Molecular bioSystems

authors： Li H,Pan JY,Liu XJ,Gao JX,Wu HK,Wang C,Peng XX

更新日期：2012-09-01 00:00:00

abstract：:Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...

journal_title：Molecular bioSystems

authors： Kang A,Tan MH,Ling H,Chang MW

更新日期：2013-02-02 00:00:00

abstract：:The recognition and association between the Ca2+/calmodulin-activated protein kinase II-α (CaMKIIα) and the multi-PDZ domain protein 1 (MUPP1) plays an important role in the sperm acrosome reaction and human fertilization. Previously, we have demonstrated that the MUPP1 PDZ11 domain is the primary binding partner of t...

journal_title：Molecular bioSystems

authors： Zhang YL,Han ZF

更新日期：2017-09-26 00:00:00

abstract：:Unbalanced uptake of Omega 6/Omega 3 (ω-6/ω-3) ratios could increase chronic disease occurrences, such as inflammation, atherosclerosis, or tumor proliferation, and methylation methods for measuring the ruminal microbiome fatty acid (FA) composition/distribution play a vital role in discovering the contribution of foo...

journal_title：Molecular bioSystems

authors： Liu Y,Buendía-Rodríguez G,Peñuelas-Rívas CG,Tan Z,Rívas-Guevara M,Tenorio-Borroto E,Munteanu CR,Pazos A,González-Díaz H

更新日期：2015-11-01 00:00:00

abstract：:Breast cancer has been reported as one of the most frequently diagnosed malignant diseases and the leading cause of cancer death in women all around the world. Furthermore, this complicated cancer is divided into multiple subtypes which present different clinical symptoms and need correspondingly directed therapy. We ...

journal_title：Molecular bioSystems

authors： Chen Y,Wang X,Wang G,Li Z,Wang J,Huang L,Qin Z,Yuan X,Cheng Z,Zhang S,Yin Y,He J

更新日期：2017-05-02 00:00:00