Nucleoside phosphocholine amphiphile for in vitro DNA transfection.

abstract：:MiRNAs are a fascinating kind of biomolecule due to their vital functions in gene regulation and potential value as biomarkers for serious diseases including cancers. Exploiting convenient and sensitive methods for miRNA expression assays is imperative. In this study, we employ an exonuclease (RecJf) and a nicking end...

journal_title：Molecular bioSystems

authors： Xu J,Han K,Liu D,Lin L,Miao P

更新日期：2016-11-15 00:00:00

abstract：:In vivo analysis is of paramount importance in monitoring physiological processes that take place in living organisms. Mass spectrometry, an analytical technique with high speed, sensitivity and specificity, is indispensable in biochemical studies nowadays. However, traditional mass spectrometric techniques are of lim...

journal_title：Molecular bioSystems

authors： So PK,Hu B,Yao ZP

更新日期：2013-05-01 00:00:00

abstract：:Although angiotensin (Ang)II-induced Janus-activated kinase (JAK)2 phosphorylation was reported to be enhanced in failing human cardiomyocytes, the downstream balance between cardio-protective (signal transducer and activator of transcription-STAT3) and the pro-inflammatory (STAT2 and STAT5) response remains unexplore...

journal_title：Molecular bioSystems

authors： Cambi GE,Lucchese G,Djeokeng MM,Modesti A,Fiaschi T,Faggian G,Sani G,Modesti PA

更新日期：2012-09-01 00:00:00

abstract：:The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...

journal_title：Molecular bioSystems

authors： Kabir A,Kumar GS

更新日期：2014-05-01 00:00:00

abstract：:The present study describes for the first time, a metabolic profile reflecting the osteoporosis progression in 364 pre- and postmenopausal Chinese women using GC-MS. In order to accurately evaluate the dynamic changes of metabolites along with estrogen deficiency and osteoporosis progression, we divided these subjects...

journal_title：Molecular bioSystems

authors： Qi H,Bao J,An G,Ouyang G,Zhang P,Wang C,Ying H,Ouyang P,Ma B,Zhang Q

更新日期：2016-06-21 00:00:00

abstract：:Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...

journal_title：Molecular bioSystems

authors： Pan X,Xiong K

更新日期：2015-08-01 00:00:00

abstract：:Electrospray ionization mass spectrometry combined with high efficiency capillary liquid chromatography provides high sensitivity and broad dynamic range measurements for the characterization of biological macromolecules in complex matrices, and is an increasingly powerful analytical tool for systems biology research....

journal_title：Molecular bioSystems

authors： Smith RD,Tang K,Shen Y

更新日期：2006-05-01 00:00:00

abstract：:Here, we used stopped-flow fluorescence techniques to conduct a comparative kinetic analysis of the conformational transitions in human apurinic/apyrimidinic endonuclease 1 (APE1) and in DNA containing an abasic site in the course of their interaction. Effects of monovalent (K(+)) and divalent (Mg(2+), Mn(2+), Ca(2+),...

journal_title：Molecular bioSystems

authors： Miroshnikova AD,Kuznetsova AA,Vorobjev YN,Kuznetsov NA,Fedorova OS

更新日期：2016-05-26 00:00:00

abstract：:CC chemokine receptor 5 (CCR5), a member of G protein-coupled receptors (GPCRs), plays a vital role in inflammatory responses to infection. Alterations in the expression of CCR5 have been correlated with disease progression in many types of cancers. The idea of using CCR5 as a target for therapeutic intervention has b...

journal_title：Molecular bioSystems

authors： Wang J,Shu M,Wang Y,Hu Y,Wang Y,Luo Y,Lin Z

更新日期：2016-10-18 00:00:00

abstract：:Regulatory networks are able to process complex signals and respond appropriately to the cellular context. Thus, an increasing effort by systems biology researchers is being focused on understanding which interactions are responsible for a given functional response. When translated into specific mathematical models, h...

journal_title：Molecular bioSystems

authors： Estrada J,Guantes R

更新日期：2013-02-02 00:00:00

abstract：:Structural insight into DNA quadruplex structures formed by oligodeoxyribonucleotides 3'TG5'-5'GGGT3' (QS55) and 5'TG3'-3'GGGT5' (QS33) is presented. NMR analysis reveals that QS33 forms a parallel-like four-fold symmetric quadruplex, while QS55 possesses a two-fold symmetry and is characterized by a tetrameric antipa...

journal_title：Molecular bioSystems

authors： Galeone A,Mayol L,Virgilio A,Virno A,Randazzo A

更新日期：2008-05-01 00:00:00

abstract：:Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather th...

journal_title：Molecular bioSystems

authors： Altman S

更新日期：2007-09-01 00:00:00

abstract：:In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...

journal_title：Molecular bioSystems

authors： Torgasheva NA,Menzorova NI,Sibirtsev YT,Rasskazov VA,Zharkov DO,Nevinsky GA

更新日期：2016-06-21 00:00:00

abstract：:RNA-binding proteins (RBPs) are key regulators of gene expression. Some long non-coding RNAs (lncRNAs) affect gene expression by interacting with RBPs. However, whether this influences the biological characteristics of lncRNAs in diseases still remains unknown. Here, we classify lncRNAs into two categories, using the ...

journal_title：Molecular bioSystems

authors： Zhang Q,Wei Y,Yan Z,Wu C,Chang Z,Zhu Y,Li K,Xu Y

更新日期：2017-06-01 00:00:00

abstract：:The dairy bacterium Lactococcus lactis has to master a complicated task. It must control its essentially linear glycolytic pathway in such a fashion that, when the substrate, glucose, runs out, it retains enough phosphoenolpyruvate and fructose-1,6-bisphosphate to be able to restart glycolysis as soon as new glucose b...

journal_title：Molecular bioSystems

authors： Dolatshahi S,Fonseca LL,Voit EO

更新日期：2016-01-01 00:00:00

abstract：:A recent model proposing that a barrier is raised against tumor evolution in pre-cancer tissues is investigated. For that we quantify expression alterations in genome maintenance pathways: DNA damage response, death pathways and cell cycle and also differentially expressed genes in transcriptomes of pre-cancerous and ...

journal_title：Molecular bioSystems

authors： Simão ÉM,Sinigaglia M,Bugs CA,Castro MA,Librelotto GR,Alves R,Mombach JC

更新日期：2012-11-01 00:00:00

abstract：:The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active doma...

journal_title：Molecular bioSystems

authors： Malik V,Zhang M,Dover LG,Northen JS,Flinn A,Perry JJ,Black GW

更新日期：2013-11-01 00:00:00

abstract：:Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...

journal_title：Molecular bioSystems

authors： Kumar KM,Anbarasu A,Ramaiah S

更新日期：2014-04-01 00:00:00

abstract：:Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...

journal_title：Molecular bioSystems

authors： Wehenkel M,Hong JT,Kim KB

更新日期：2008-04-01 00:00:00

abstract：:A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity t...

journal_title：Molecular bioSystems

authors： Samanfar B,Omidi K,Hooshyar M,Laliberte B,Alamgir M,Seal AJ,Ahmed-Muhsin E,Viteri DF,Said K,Chalabian F,Golshani A,Wainer G,Burnside D,Shostak K,Bugno M,Willmore WG,Smith ML,Golshani A

更新日期：2013-06-01 00:00:00

abstract：:The pro-inflammatory cytokines interleukin 1 (IL-1) and 6 (IL-6) are crucially involved in the regulation of a multitude of physiological processes, in particular coordinating the immune response upon bacterial infection and tissue injury. Both interleukins induce complex signalling cascades and trigger the production...

journal_title：Molecular bioSystems

authors： Ryll A,Samaga R,Schaper F,Alexopoulos LG,Klamt S

更新日期：2011-12-01 00:00:00

abstract：:Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO˙, ˙O(2)(-) and ˙OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO˙, HPLC-MS aided PC12 cell systems were developed, and the conversions of 2-...

journal_title：Molecular bioSystems

authors： Wang Y,Jiang X,Liu J,Zhao M,Kang G,Wu J,Peng L,Peng S

更新日期：2011-05-01 00:00:00

abstract：:Breast cancer has been reported as one of the most frequently diagnosed malignant diseases and the leading cause of cancer death in women all around the world. Furthermore, this complicated cancer is divided into multiple subtypes which present different clinical symptoms and need correspondingly directed therapy. We ...

journal_title：Molecular bioSystems

authors： Chen Y,Wang X,Wang G,Li Z,Wang J,Huang L,Qin Z,Yuan X,Cheng Z,Zhang S,Yin Y,He J

更新日期：2017-05-02 00:00:00

abstract：:In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....

journal_title：Molecular bioSystems

authors： Eltoweissy M,Dihazi GH,Müller GA,Asif AR,Dihazi H

更新日期：2016-05-24 00:00:00

abstract：:Normalization of array data relies on the assumption that most genes are not altered, which means that the signals for different samples should be scaled to have similar median or average values. However, accumulating evidence suggests that gene expression could be widely up-regulated in cancers. Our previous results ...

journal_title：Molecular bioSystems

authors： Wu D,Kang J,Huang Y,Li X,Wang X,Huang D,Wang Y,Li B,Hao D,Gu Q,Tang N,Li K,Guo Z,Li X,Xu J,Wang D

更新日期：2014-06-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are key signaling proteins that regulate how cells interact with their environment. Traditional signaling cascades involving GPCRs have been well described and are well established and very important clinical targets. With the development of more recent technologies, hints about the...

journal_title：Molecular bioSystems

authors： Zhang X,Eggert US

更新日期：2013-04-05 00:00:00

abstract：:Small molecule modulators are critical for dissecting and understanding signaling pathways at the molecular level. Interleukin 6 (IL-6) is a cytokine that signals via the JAK-STAT pathway and is implicated in cancer and inflammation. To identify modulators of this pathway, we screened a chemical collection against an ...

journal_title：Molecular bioSystems

authors： Johnson RL,Huang R,Jadhav A,Southall N,Wichterman J,MacArthur R,Xia M,Bi K,Printen J,Austin CP,Inglese J

更新日期：2009-09-01 00:00:00

abstract：:Cellular heterogeneity and stochastic fluctuation play key roles in biological processes. Single molecule approaches have the key advantage of avoiding ensemble averaging, allowing the observation of transient intermediates and heterogeneity (both static and dynamic). Thus they have revolutionised the way many biologi...

journal_title：Molecular bioSystems

authors： Ying L

更新日期：2007-06-01 00:00:00

abstract：:Upon rapid ascent to a high altitude, non-acclimatized individuals, although healthy, are highly prone to contracting high-altitude pulmonary edema (HAPE). Early diagnosis is difficult and there is no reliable biomarker available. We used proton ((1)H) NMR metabolomics to profile the altered metabolic patterns of bloo...

journal_title：Molecular bioSystems

authors： Luo Y,Zhu J,Gao Y

更新日期：2012-06-01 00:00:00

abstract：:Proteomics is the study of proteins and their interactions within complex biological systems. While this field is often associated with mass spectrometry, it is more useful to consider proteomics in the context of an objective: to identify and understand the molecular basis of health and disease at the protein level i...

journal_title：Molecular bioSystems

authors： Bark SJ,Hook V

更新日期：2007-01-01 00:00:00