Abstract:
:Electrospray ionization mass spectrometry combined with high efficiency capillary liquid chromatography provides high sensitivity and broad dynamic range measurements for the characterization of biological macromolecules in complex matrices, and is an increasingly powerful analytical tool for systems biology research.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Smith RD,Tang K,Shen Ydoi
10.1039/b601468bsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
221-30issue
5eissn
1742-206Xissn
1742-2051journal_volume
2pub_type
杂志文章,评审abstract::The adaptation of the cellular network to functional changes, timing and patterning of gene expression is regulated by binding of transcription factors to gene regulatory elements, which in turn depends on co-occurring histone modifications. These two layers influence each other, enabling a further level of regulatory...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05363a
更新日期:2012-02-01 00:00:00
abstract::Ca2+ transport across the sarco/endoplasmic reticulum (SR) plays an essential role in intracellular Ca2+ homeostasis, signalling, cell differentiation and muscle contractility. During SR Ca2+ uptake and release, proton fluxes are required to balance the charge deficit generated by the exchange of Ca2+ and other ions a...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c7mb00065k
更新日期:2017-03-28 00:00:00
abstract::The advent of large scale sequencing methods has enabled analyses of the protein-coding parts of cancer genomes to find the mutated genes that cause common human cancers. Unbiased mutation analyses of human tumors originating in the breast, colon, brain, and pancreas have revealed genomic landscapes composed of a few ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b903502h
更新日期:2009-09-01 00:00:00
abstract::To understand the regulatory dynamics of transcription factors (TFs) and their interplay with other cellular components we have integrated transcriptional, protein-protein and the allosteric or equivalent interactions which mediate the physiological activity of TFs in Escherichia coli. To study this integrated network...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25279a
更新日期:2012-11-01 00:00:00
abstract::The underlying reasons for the differences in the virulence of various types of Ebola virus (EBOV) remain unknown. Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of EBOV. The higher disorder of these pro...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00240k
更新日期:2015-08-01 00:00:00
abstract::Epithelial ovarian cancer (EOC) is the most lethal of gynecologic malignancies due to the high rate of recurrence and poor prognosis. Predicting the prognosis in patients with EOC is clinically challenging, partly because appropriate biomarkers of recurrence have yet to be explored. In this prospective study, pre-trea...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00407h
更新日期:2015-02-01 00:00:00
abstract::Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00339e
更新日期:2011-04-01 00:00:00
abstract::Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b801245h
更新日期:2008-07-01 00:00:00
abstract::RNA-binding proteins (RBPs) are key regulators of gene expression. Some long non-coding RNAs (lncRNAs) affect gene expression by interacting with RBPs. However, whether this influences the biological characteristics of lncRNAs in diseases still remains unknown. Here, we classify lncRNAs into two categories, using the ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00144d
更新日期:2017-06-01 00:00:00
abstract::Ligand-induced conditional protein splicing (CPS) using a split intein allows the covalent reconstitution of a protein from two polypeptide fragments. The small molecule rapamycin binds to the fused FKBP and FRB dimerizer domains and thereby induces folding of the split intein, which then removes itself in the trans-s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05025g
更新日期:2011-06-01 00:00:00
abstract::Unstable angina (UA) is one of the most dangerous types of coronary heart disease and has high mortality and morbidity rates worldwide. However, the diagnostic accuracy for UA is unsatisfactory in clinical practice. In this study, we investigated the application of plasma metabolomics in discovering potential biomarke...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70216b
更新日期:2013-12-01 00:00:00
abstract::Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for recombinant proteins. However, in vitro methods for Drosophila cell and organ c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00155a
更新日期:2014-10-01 00:00:00
abstract::MicroiRNAs are genome encoded small double stranded RNAs that regulate expression of homologous mRNAs. With approximately 2500 human miRNAs and each having hundreds of potential mRNA targets, miRNA based gene regulation is quite pervasive in both development and disease. While there are numerous studies investigating ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00415b
更新日期:2016-01-01 00:00:00
abstract::Peptidylarginine deiminase (PAD) catalyzes the posttranslational citrullination of selected proteins in a calcium dependent manner. The PAD4 isoform has been implicated in multiple sclerosis, rheumatoid arthritis, some types of cancer, and plays a role in gene regulation. However, the substrate selectivity of PAD4 is ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05089c
更新日期:2011-07-01 00:00:00
abstract::As one of the most important trace elements within an organism, zinc has been shown to be involved in numerous biological processes and closely implicated in various diseases. The zinc ion is important for proteins to perform their functional roles. To provide in-depth functional annotation of zinc-binding proteins, a...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70100j
更新日期:2013-09-01 00:00:00
abstract::Human mitogen-activated protein kinase (MAPK) family members JNK and p38 are two homologous protein-serine/threonine kinases but play distinct roles in the pathological process of neurological disorders. Selective targeting of JNK over p38 has been established as a potential therapeutic approach to epilepsy and other ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00297h
更新日期:2016-07-19 00:00:00
abstract::High levels of DNA and RNA released by apoptotic and necrotic cells circulate in the blood of cancer patients. In the present study we determined the applicability of the quantification of nucleic acids and their genetic alterations as minimally invasive tool for breast cancer screening. The relative concentrations of...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05197k
更新日期:2011-10-01 00:00:00
abstract::The major mechanical function of talin is to couple the β-integrin cytoplasmic tails to actin filaments. A variety of β-integrin tails contain conserved binding motifs for talin, and recent research shows that β-integrins differ both in affinity to talin and preferences for other cytoplasmic adaptor proteins. While ta...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00341a
更新日期:2014-12-01 00:00:00
abstract::Rhein is a natural product purified from herbal plants such as Rheum palmatum, which has been shown to have anti-angiogenesis and anti-tumor metastasis properties. However, the biological effects of rhein on the behavior of breast cancers are not completely elucidated. To evaluate whether rhein might be useful in the ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00451e
更新日期:2014-12-01 00:00:00
abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::Drug combinations have been widely applied to treat complex diseases, like cancer, HIV and cardiovascular diseases. One of the most important characteristics for drug combinations is the synergistic effects among different drugs, that is to say, the combination effects are larger than the sum of individual effects. Al...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00599j
更新日期:2016-02-01 00:00:00
abstract::Many methods have been published by which combinatorial libraries may be screened for compounds capable of manipulating the function(s) of a target protein. One of the simplest approaches is to identify compounds in a library that bind the protein of interest, since these binding events usually occur on functionally i...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b514349g
更新日期:2006-01-01 00:00:00
abstract::The size and complexity of cellular systems make building predictive models an extremely difficult task. In principle dynamical time-course data can be used to elucidate the structure of the underlying molecular mechanisms, but a central and recurring problem is that many and very different models can be fitted to exp...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00107d
更新日期:2011-05-01 00:00:00
abstract::A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b503302k
更新日期:2005-09-01 00:00:00
abstract::Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00642b
更新日期:2016-03-01 00:00:00
abstract::In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00887e
更新日期:2016-05-24 00:00:00
abstract::Information on extracellular signals and conditions is often transduced by biological systems using cascades of protein phosphorylation that affect the activity of enzymes, the localization of proteins and gene expression. A model to study signal transduction is the response of the yeast Saccharomyces cerevisiae to os...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b902256b
更新日期:2009-11-01 00:00:00
abstract::4-Hydroxyderricin is a heat labile bioactive chalcone isolated from the plant Angelica keiskei. It received attention due to its antibiotic potency against several strains of bacteria including pathogens such as Staphylococcus aureus. Despite these promising pharmacological properties, the exact mode of action or the ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25446h
更新日期:2013-03-01 00:00:00
abstract::The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00071h
更新日期:2015-06-01 00:00:00
abstract::The emergence of drug resistant strains of Mycobacterium Tuberculosis (Mtb) accentuates the urgent need for the development of novel antitubercular drugs. The major causes of drug resistance are genetic mutations, the influx-efflux transporter system, and the complex cell wall system of Mtb, which can function as perm...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00457a
更新日期:2016-10-18 00:00:00