The Ca2+-ATPase pump facilitates bidirectional proton transport across the sarco/endoplasmic reticulum.

abstract：:RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe t...

journal_title：Molecular bioSystems

authors： Utku Y,Dehan E,Ouerfelli O,Piano F,Zuckermann RN,Pagano M,Kirshenbaum K

更新日期：2006-06-01 00:00:00

abstract：:Synthetic studies of lipid A and LPS partial structures have been performed to investigate the relationship between structures and functions of LPS. Recent studies have suggested several pathological implications of LPS from parasitic bacteria due to its influence on the host immune responses. To address this issue, w...

journal_title：Molecular bioSystems

authors： Fujimoto Y,Shimoyama A,Saeki A,Kitayama N,Kasamatsu C,Tsutsui H,Fukase K

更新日期：2013-05-01 00:00:00

abstract：:Non-ribosomal peptide synthetases (NRPSs) are enzymes that catalyze ribosome-independent production of small peptides, most of which are bioactive. NRPSs act as peptide assembly lines where individual, often interconnected modules each incorporate a specific amino acid into the nascent chain. The modules themselves co...

journal_title：Molecular bioSystems

authors： Beer R,Herbst K,Ignatiadis N,Kats I,Adlung L,Meyer H,Niopek D,Christiansen T,Georgi F,Kurzawa N,Meichsner J,Rabe S,Riedel A,Sachs J,Schessner J,Schmidt F,Walch P,Niopek K,Heinemann T,Eils R,Di Ventura B

更新日期：2014-07-01 00:00:00

abstract：:Recently, the development of Src/Abl (c-Src/Bcr-Abl tyrosine kinases) dual inhibitors has attracted considerable attention from the research community for treatment of malignancies. In order to explore the different structural features impacting the Src and Abl inhibitory activities of N(9)-arenethenyl purines and to ...

journal_title：Molecular bioSystems

authors： Ma S,Zeng G,Fang D,Wang J,Wu W,Xie W,Tan S,Zheng K

更新日期：2015-02-01 00:00:00

abstract：:The Library of Integrated Network-based Cellular Signatures (LINCS) L1000 big data provide gene expression profiles induced by over 10 000 compounds, shRNAs, and kinase inhibitors using the L1000 platform. We developed csNMF, a systematic compound signature discovery pipeline covering from raw L1000 data processing to...

journal_title：Molecular bioSystems

authors： Liu C,Su J,Yang F,Wei K,Ma J,Zhou X

更新日期：2015-03-01 00:00:00

abstract：:Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...

journal_title：Molecular bioSystems

authors： Lowther J,Yard BA,Johnson KA,Carter LG,Bhat VT,Raman MC,Clarke DJ,Ramakers B,McMahon SA,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Proteins are dynamic creatures. Intrinsically disordered proteins (IDPs) function as multiplicity of structures and their activities can only be described by stochastic structure-function relationships. In their complex forms, however, IDPs were thought to lose their plasticity and behave similarly to globular protein...

journal_title：Molecular bioSystems

authors： Fuxreiter M

更新日期：2012-01-01 00:00:00

abstract：:The glutamate γ-carboxylation plays a pivotal part in a number of important human diseases. However, traditional protein γ-carboxylation site detection by experimental approaches are often laborious and time-consuming. In this study, we initiated an attempt for the computational prediction of protein γ-carboxylation s...

journal_title：Molecular bioSystems

authors： Zhang N,Li BQ,Gao S,Ruan JS,Cai YD

更新日期：2012-11-01 00:00:00

abstract：:Ebola virus (EBOV) is highly lethal due to virally encoded immune antagonists, and the combination of EBOV VP24 with karyopherin alpha (KPNA) will trigger anti-interferon (IFN) signaling. The crystal structure of VP24-KPNA5 has been proposed in recent studies, but the precise binding mechanisms are still unclear. In o...

journal_title：Molecular bioSystems

authors： Ding JN,Zhang YJ,Zhong H,Ao CC,Li J,Han JG

更新日期：2017-05-02 00:00:00

abstract：:Recently, accumulating studies have indicated that microRNAs (miRNAs) play an important role in exploring the pathogenesis of various human diseases at the molecular level and may result in the design of specific tools for diagnosis, treatment evaluation and prevention. Experimental identification of disease-related m...

journal_title：Molecular bioSystems

authors： Sun D,Li A,Feng H,Wang M

更新日期：2016-06-21 00:00:00

abstract：:Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsic...

journal_title：Molecular bioSystems

authors： Milles S,Koehler C,Gambin Y,Deniz AA,Lemke EA

更新日期：2012-10-01 00:00:00

abstract：:Schistosomiasis is a parasitic infection that is endemic in many developing countries in the tropics and subtropics afflicting more than 207 million people primarily in rural areas. After malaria, it is the second most important parasitic infection in terms of socio-economic and public health. Investigation of the hos...

journal_title：Molecular bioSystems

authors： Balog CI,Meissner A,Göraler S,Bladergroen MR,Vennervald BJ,Mayboroda OA,Deelder AM

更新日期：2011-05-01 00:00:00

abstract：:During the preclinical drug discovery process it remains a challenge to enable early elimination of candidate molecules that may have non-specific, off-target activities. Here, we use whole zebrafish embryo assays coupled with genetic analysis to address this issue. PLK1 (Polo-like kinase 1) is one of the key regulato...

journal_title：Molecular bioSystems

authors： Zhong H,Xin S,Zhao Y,Lu J,Li S,Gong J,Yang Z,Lin S

更新日期：2010-08-01 00:00:00

abstract：:Integrative pharmacology has been used to identify the key active constituents (KACs) of Buchang Naoxintong capsules (BNCs), a traditional Chinese medical preparation; this approach involves the evaluation of the content profiles and drug-like properties of the BNC constituents and development of an ingredient-target ...

journal_title：Molecular bioSystems

authors： Haiyu X,Yang S,Yanqiong Z,Qiang J,Defeng L,Yi Z,Feng L,Hongjun Y

更新日期：2016-01-01 00:00:00

abstract：:CC chemokine receptor 5 (CCR5), a member of G protein-coupled receptors (GPCRs), plays a vital role in inflammatory responses to infection. Alterations in the expression of CCR5 have been correlated with disease progression in many types of cancers. The idea of using CCR5 as a target for therapeutic intervention has b...

journal_title：Molecular bioSystems

authors： Wang J,Shu M,Wang Y,Hu Y,Wang Y,Luo Y,Lin Z

更新日期：2016-10-18 00:00:00

abstract：:Epithelial to mesenchymal transition (EMT) is a biological process that plays a crucial role in cancer metastasis. Although studies regarding the EMT mechanisms are usual in terms of gene expression and protein functions, little is known about the involvement of lipids in EMT. In this work, an untargeted lipidomic ana...

journal_title：Molecular bioSystems

authors： Dalmau N,Jaumot J,Tauler R,Bedia C

更新日期：2015-12-01 00:00:00

abstract：:Calcium homeostasis is a key factor in the regulation of cardiac excitation-contraction coupling. Calcium dynamics in cardiomyocytes is governed by ATP which depends on insulin dependent glucose concentration, via the glucose transporter type 4 (GLUT4) transporter. It would therefore be interesting to see how calcium ...

journal_title：Molecular bioSystems

authors： Das PN,Kumar A,Bairagi N,Chatterjee S

更新日期：2017-09-26 00:00:00

abstract：:α-Anomers of 2'-deoxyadenosine (αdA) are major products of deoxyadenosine damage when DNA is γ-irradiated under anoxic conditions. Such lesions are a threat to genomic stability and are known to be processed by human apurinic/apyrimidinic endonuclease 1 (APE1). The aim of this study was to determine whether the α-anom...

journal_title：Molecular bioSystems

authors： Timofeyeva NA,Fedorova OS

更新日期：2016-10-18 00:00:00

abstract：:We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6...

journal_title：Molecular bioSystems

authors： Wagstaff JL,Sadovskaya I,Vinogradov E,Jabbouri S,Howard MJ

更新日期：2008-02-01 00:00:00

abstract：:Asthma is a chronic inflammatory lung disease that leads to 250 000 deaths annually. There is a need to better understand asthma by identifying new pathogenic molecules. We conducted a liquid-chromatography time-of-flight mass spectrometry (LC-Q-TOF-MS)-based metabolomics study to test for asthma and investigate the i...

journal_title：Molecular bioSystems

authors： Su L,Shi L,Liu J,Huang L,Huang Y,Nie X

更新日期：2017-05-30 00:00:00

abstract：:To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...

journal_title：Molecular bioSystems

authors： Nikolov M,Fischle W

更新日期：2013-02-02 00:00:00

abstract：:Chronic inflammation can lead to the development of cancers and resolution of inflammation is an ongoing challenge. Inflammation can result from dysregulation of the epigenome and a number of compounds that modify the epigenome are in clinical use. In this study the anti-inflammatory and anti-cancer effects of a quina...

journal_title：Molecular bioSystems

authors： Lin Z,Bishop KS,Sutherland H,Marlow G,Murray P,Denny WA,Ferguson LR

更新日期：2016-03-01 00:00:00

abstract：:Protein-protein interactions are important for the majority of biological processes. A significant number of computational methods have been developed to predict protein-protein interactions using protein sequence, structural and genomic data. Vast experimental data is publicly available on the Internet, but it is sca...

journal_title：Molecular bioSystems

authors： Saha I,Zubek J,Klingström T,Forsberg S,Wikander J,Kierczak M,Maulik U,Plewczynski D

更新日期：2014-04-01 00:00:00

abstract：:Osteosarcoma (OS) is a highly malignant bone tumour, affecting mainly children and young adults between 10 and 20 years of age. It represents the most frequent primitive malignant tumour of the skeletal system and is characterized by an extremely aggressive clinical course, with rapid development of lung metastases. I...

journal_title：Molecular bioSystems

authors： Bernardini G,Laschi M,Serchi T,Spreafico A,Botta M,Schenone S,Arena S,Geminiani M,Scaloni A,Collodel G,Orlandini M,Niccolai N,Santucci A

更新日期：2014-06-01 00:00:00

abstract：:Climatic change is an increasing challenge for agriculture that is driving the development of suitable crops in order to ensure supply for both human nutrition and animal feed. In this context, it is increasingly important to understand the biochemical responses of cells to environmental cues at the whole system level...

journal_title：Molecular bioSystems

authors： García-Flores M,Juárez-Colunga S,Montero-Vargas JM,López-Arciniega JA,Chagolla A,Tiessen A,Winkler R

更新日期：2012-06-01 00:00:00

abstract：:Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...

journal_title：Molecular bioSystems

authors： Li S,Wang Y,Zhang J,Yang WH,Dai ZH,Zhu W,Yu XQ

更新日期：2011-04-01 00:00:00

abstract：:Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...

journal_title：Molecular bioSystems

authors： Lu B,Sun YX,Li YQ,Zhang XZ,Zhuo RX

更新日期：2009-06-01 00:00:00

abstract：:Insights into the structure and dynamics of modular polyketide synthases (PKS) are essential for understanding the mechanistic details of the biosynthesis of a large number of pharmaceutically important secondary metabolites. The crystal structures of the KS-AT di-domain from erythromycin synthase have revealed the re...

journal_title：Molecular bioSystems

authors： Anand S,Mohanty D

更新日期：2012-04-01 00:00:00

abstract：:Lamellipodin (Lpd) protein plays an important role in the formation of lamellipodial protrusion which is crucial in actin dynamics, cell polarity and motility. Lpd promotes actin polymerization with the help of members of the Ena/VASP family of actin regulators and tethering them to actin filaments. It is well documen...

journal_title：Molecular bioSystems

authors： Gorai S,Paul D,Haloi N,Borah R,Santra MK,Manna D

更新日期：2016-03-01 00:00:00

abstract：:The aim of this study was to investigate the cellular lipid metabolism associated with β-amyloid peptide (Aβ)-induced neurotoxicity as well as the neuroprotective effect of (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea. An ultra-performance liquid chromatography-quadrupole-time-of-flight mass sp...

journal_title：Molecular bioSystems

authors： Zhang H,Wang JR,Yau LF,Ho HM,Chan CL,Hu P,Liu L,Jiang ZH

更新日期：2012-10-30 00:00:00