abstract：:Infectious diseases caused by bacterial pathogens pose a major concern to public health and, thus, greater attention must be given to providing insightful knowledge on host-pathogen interactions. There are several theories addressing the dynamics of complex mechanisms of host-pathogen interactions. The availability of...

journal_title：Molecular bioSystems

authors： Kamaladevi A,Marudhupandiyan S,Balamurugan K

更新日期：2017-11-21 00:00:00

abstract：:Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur, which compromise the experimental results. In...

journal_title：Molecular bioSystems

authors： Suttapitugsakul S,Xiao H,Smeekens J,Wu R

更新日期：2017-11-21 00:00:00

abstract：:Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies d...

journal_title：Molecular bioSystems

authors： Ven Chang I,Tsutsumi H,Mihara H

更新日期：2017-10-24 00:00:00

abstract：:Genome sequencing technology has generated a vast amount of genomic and epigenomic data, and has provided us a great opportunity to study gene functions on a global scale from an epigenomic view. In the last decade, network-based studies, such as those based on PPI networks and co-expression networks, have shown good ...

journal_title：Molecular bioSystems

authors： Li W,Wang M,Sun J,Wang Y,Jiang R

更新日期：2017-10-24 00:00:00

abstract：:TGF-β1, 2 and 3 cytokines are involved in many cellular processes including cell proliferation, differentiation, migration and survival. Whereas TGF-β2 and 3 play important roles in embryonic development, TGF-β1 is mostly implicated in controlling immune responses after birth. The production of TGF-β1 is a tightly reg...

journal_title：Molecular bioSystems

authors： Stockis J,Dedobbeleer O,Lucas S

更新日期：2017-09-26 00:00:00

abstract：:Calcium homeostasis is a key factor in the regulation of cardiac excitation-contraction coupling. Calcium dynamics in cardiomyocytes is governed by ATP which depends on insulin dependent glucose concentration, via the glucose transporter type 4 (GLUT4) transporter. It would therefore be interesting to see how calcium ...

journal_title：Molecular bioSystems

authors： Das PN,Kumar A,Bairagi N,Chatterjee S

更新日期：2017-09-26 00:00:00

abstract：:The multidrug resistance (MDR) system actively pumps antibiotics out of cells causing serious health problems. During the pumping, AcrB (one of the key components of MDR) undergoes a series of large-scale and proton-motive conformational changes. Capturing the conformational changes through all-atom simulations is cha...

journal_title：Molecular bioSystems

authors： Jewel Y,Liu J,Dutta P

更新日期：2017-09-26 00:00:00

abstract：:Lung fibrosis is a progressive and often fatal lung disease characterized by fibroblast proliferation and excessive deposition of extracellular matrix in the lungs. The chemokine receptor CXCR7 has been shown to control cell adhesion, migration and proliferation by regulating the epithelial-to-mesenchymal transition (...

journal_title：Molecular bioSystems

authors： Guan S,Zhou J

更新日期：2017-09-26 00:00:00

abstract：:The recognition and association between the Ca2+/calmodulin-activated protein kinase II-α (CaMKIIα) and the multi-PDZ domain protein 1 (MUPP1) plays an important role in the sperm acrosome reaction and human fertilization. Previously, we have demonstrated that the MUPP1 PDZ11 domain is the primary binding partner of t...

journal_title：Molecular bioSystems

authors： Zhang YL,Han ZF

更新日期：2017-09-26 00:00:00

abstract：:One of the fundamental goals in cellular biochemistry is to identify the functions of proteins in the context of compartments that organize them in the cellular environment. To realize this, it is indispensable to develop an automated method for fast and accurate identification of the subcellular locations of uncharac...

journal_title：Molecular bioSystems

authors： Cheng X,Xiao X,Chou KC

更新日期：2017-08-22 00:00:00

abstract：:The major obstacle for the development of targeted therapies is the lack of pharmacodynamic (PD) biomarkers to provide an early readout of biological activities. As the modulation of metabolites may reflect the biological changes occurring in the targets, metabolomics is promising to be an efficient way to explore PD ...

journal_title：Molecular bioSystems

authors： Fang J,Wang L,Wang Y,Qiu M,Zhang Y

更新日期：2017-07-25 00:00:00

abstract：:RNA-binding proteins (RBPs) are key regulators of gene expression. Some long non-coding RNAs (lncRNAs) affect gene expression by interacting with RBPs. However, whether this influences the biological characteristics of lncRNAs in diseases still remains unknown. Here, we classify lncRNAs into two categories, using the ...

journal_title：Molecular bioSystems

authors： Zhang Q,Wei Y,Yan Z,Wu C,Chang Z,Zhu Y,Li K,Xu Y

更新日期：2017-06-01 00:00:00

abstract：:Photo-chemical deamination of cytosine using 3-cyanovinylcarbazole nucleoside (CNVK) mediated photo-cross-linking is a technique for site-directed mutagenesis. Using this technique in vivo requires the elimination of a high-temperature incubation step; instead, incubation should be carried out under physiological cond...

journal_title：Molecular bioSystems

authors： Sethi S,Ooe M,Sakamoto T,Fujimoto K

更新日期：2017-06-01 00:00:00

abstract：:The ectonucleotide phosphodiesterase/pyrophosphatase-1 (NPP1) is a type II transmembrane glycoprotein that regulates extracellular inorganic purine nucleotide and inorganic diphosphate levels through the hydrolysis of ATP into AMP and diphosphate. NPP1 is a promising drug target as it plays a role in several disorders...

journal_title：Molecular bioSystems

authors： Barbeau X,Mathieu P,Paquin JF,Lagüe P

更新日期：2017-05-30 00:00:00

abstract：:Asthma is a chronic inflammatory lung disease that leads to 250 000 deaths annually. There is a need to better understand asthma by identifying new pathogenic molecules. We conducted a liquid-chromatography time-of-flight mass spectrometry (LC-Q-TOF-MS)-based metabolomics study to test for asthma and investigate the i...

journal_title：Molecular bioSystems

authors： Su L,Shi L,Liu J,Huang L,Huang Y,Nie X

更新日期：2017-05-30 00:00:00

abstract：:Blockage of the interactions between immunologic checkpoint protein PD-1 and its ligand PD-L1 showed efficacy for cancer treatment. X-ray structures have captured static conformational snapshots of PD-1 and revealed that the CC' loop adopts an open conformation in the apo-protein but turns into a closed form and inter...

journal_title：Molecular bioSystems

authors： Liu W,Huang B,Kuang Y,Liu G

更新日期：2017-05-02 00:00:00

abstract：:Ebola virus (EBOV) is highly lethal due to virally encoded immune antagonists, and the combination of EBOV VP24 with karyopherin alpha (KPNA) will trigger anti-interferon (IFN) signaling. The crystal structure of VP24-KPNA5 has been proposed in recent studies, but the precise binding mechanisms are still unclear. In o...

journal_title：Molecular bioSystems

authors： Ding JN,Zhang YJ,Zhong H,Ao CC,Li J,Han JG

更新日期：2017-05-02 00:00:00

abstract：:Breast cancer has been reported as one of the most frequently diagnosed malignant diseases and the leading cause of cancer death in women all around the world. Furthermore, this complicated cancer is divided into multiple subtypes which present different clinical symptoms and need correspondingly directed therapy. We ...

journal_title：Molecular bioSystems

authors： Chen Y,Wang X,Wang G,Li Z,Wang J,Huang L,Qin Z,Yuan X,Cheng Z,Zhang S,Yin Y,He J

更新日期：2017-05-02 00:00:00

abstract：:Genetic information in genomes is ordered, arranged in such a way that it constitutes a code, the so-called cis regulatory code. The regulatory machinery of the cell, termed trans-factors, decodes and expresses this information. In this way, genomes maintain a potential repertoire of genetic programs, parts of which a...

journal_title：Molecular bioSystems

authors： Rangel-Chavez C,Galan-Vasquez E,Martinez-Antonio A

更新日期：2017-03-28 00:00:00

abstract：:Ca2+ transport across the sarco/endoplasmic reticulum (SR) plays an essential role in intracellular Ca2+ homeostasis, signalling, cell differentiation and muscle contractility. During SR Ca2+ uptake and release, proton fluxes are required to balance the charge deficit generated by the exchange of Ca2+ and other ions a...

journal_title：Molecular bioSystems

authors： Espinoza-Fonseca LM

更新日期：2017-03-28 00:00:00

abstract：:In order to elucidate the effect of flexible linker length on the catalytic efficiency of fusion proteins, two short flexible peptide linkers of various lengths were fused between Arabidopsis thaliana 4-coumaroyl-CoA ligase (4CL) and Polygonum cuspidatum stilbene synthase (STS) to generate fusion proteins 4CL-(GSG)n-S...

journal_title：Molecular bioSystems

authors： Guo H,Yang Y,Xue F,Zhang H,Huang T,Liu W,Liu H,Zhang F,Yang M,Liu C,Lu H,Zhang Y,Ma L

更新日期：2017-02-28 00:00:00

abstract：:The essential idea of regenerative medicine is to fix or replace tissues or organs with alive and patient-specific implants. Pluripotent stem cells are able to indefinitely self-renew and differentiate into all cell types of the body which makes them a potent substantial player in regenerative medicine. The easily acc...

journal_title：Molecular bioSystems

authors： Baranek M,Belter A,Naskręt-Barciszewska MZ,Stobiecki M,Markiewicz WT,Barciszewski J

更新日期：2017-01-31 00:00:00

abstract：:Tankyrases (TNKS), key transmitters in the Wnt signaling pathway which is very conservative in evolution, are vital targets as they are overexpressed widely in many cancers. In this work, 5 inhibitors with novel structures have been discovered and validated using the ligand-based (pharmacophore) virtual screening, doc...

journal_title：Molecular bioSystems

authors： Pu Y,Zhang S,Chang Z,Zhang Y,Wang D,Zhang L,Li Y,Zuo Z

更新日期：2017-01-31 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:Understanding the complexity of cancer biology requires extensive information about the cancer proteome over the course of the disease. The recent advances in mass spectrometry-based proteomics technologies have led to the accumulation of an incredible amount of such proteomic information. This information allows us t...

journal_title：Molecular bioSystems

authors： Miah S,Banks CA,Adams MK,Florens L,Lukong KE,Washburn MP

更新日期：2016-12-20 00:00:00

abstract：:MiRNAs are a fascinating kind of biomolecule due to their vital functions in gene regulation and potential value as biomarkers for serious diseases including cancers. Exploiting convenient and sensitive methods for miRNA expression assays is imperative. In this study, we employ an exonuclease (RecJf) and a nicking end...

journal_title：Molecular bioSystems

authors： Xu J,Han K,Liu D,Lin L,Miao P

更新日期：2016-11-15 00:00:00

abstract：:Biological networks are effective tools for studying molecular interactions. Modular structure, in which genes or proteins may tend to be associated with functional modules or protein complexes, is a remarkable feature of biological networks. Mining modular structure from biological networks enables us to focus on a s...

journal_title：Molecular bioSystems

authors： Jiao QJ,Huang Y,Shen HB

更新日期：2016-11-15 00:00:00

abstract：:DNA-binding proteins play a pivotal role in various biological activities. Identification of DNA-binding residues (DBRs) is of great importance for understanding the mechanism of gene regulations and chromatin remodeling. Most traditional computational methods usually construct their predictors on static non-redundant...

journal_title：Molecular bioSystems

authors： Chai H,Zhang J,Yang G,Ma Z

更新日期：2016-11-15 00:00:00

abstract：:The biological processing - mechanism of cellular uptake, effects on the cytoplasmic and mitochondrial membranes, intracellular sites of localisation and induction of reactive oxygen species - of two dinuclear polypyridylruthenium(ii) complexes has been examined in three eukaryotic cells lines. Flow cytometry was used...

journal_title：Molecular bioSystems

authors： Li X,Heimann K,Dinh XT,Keene FR,Collins JG

更新日期：2016-10-20 00:00:00

abstract：:MicroRNAs are a predominant type of small non-coding RNAs approximately 21 nucleotides in length that play an essential role at the post-transcriptional level by either RNA degradation, translational repression or both through an RNA-induced silencing complex. Identification of these molecules can aid the dissecting o...

journal_title：Molecular bioSystems

authors： Yao Y,Ma C,Deng H,Liu Q,Zhang J,Yi M

更新日期：2016-10-20 00:00:00

abstract：:α-Anomers of 2'-deoxyadenosine (αdA) are major products of deoxyadenosine damage when DNA is γ-irradiated under anoxic conditions. Such lesions are a threat to genomic stability and are known to be processed by human apurinic/apyrimidinic endonuclease 1 (APE1). The aim of this study was to determine whether the α-anom...

journal_title：Molecular bioSystems

authors： Timofeyeva NA,Fedorova OS

更新日期：2016-10-18 00:00:00

abstract：:The emergence of drug resistant strains of Mycobacterium Tuberculosis (Mtb) accentuates the urgent need for the development of novel antitubercular drugs. The major causes of drug resistance are genetic mutations, the influx-efflux transporter system, and the complex cell wall system of Mtb, which can function as perm...

journal_title：Molecular bioSystems

authors： Janardhan S,Ram Vivek M,Narahari Sastry G

更新日期：2016-10-18 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is one of the most commonly occurring metabolic and endocrinological disorders affecting women of reproductive age. Metabolomics is an emerging field that holds promise in understanding disease pathophysiology. Recently, a few metabolomics based studies have been attempted in PCOS pati...

journal_title：Molecular bioSystems

authors： RoyChoudhury S,Mishra BP,Khan T,Chattopadhayay R,Lodh I,Datta Ray C,Bose G,Sarkar HS,Srivastava S,Joshi MV,Chakravarty B,Chaudhury K

更新日期：2016-10-18 00:00:00

abstract：:CC chemokine receptor 5 (CCR5), a member of G protein-coupled receptors (GPCRs), plays a vital role in inflammatory responses to infection. Alterations in the expression of CCR5 have been correlated with disease progression in many types of cancers. The idea of using CCR5 as a target for therapeutic intervention has b...

journal_title：Molecular bioSystems

authors： Wang J,Shu M,Wang Y,Hu Y,Wang Y,Luo Y,Lin Z

更新日期：2016-10-18 00:00:00

abstract：:We employed high-temperature classical molecular dynamics (MD) simulations to investigate the unfolding process of the wild-type (WT) and F508del-NBD1 domains of CFTR protein, with and without second-site mutations. To rationalize the in vitro behavior of F508del-NBD1, namely its lower folding yield and higher aggrega...

journal_title：Molecular bioSystems

authors： Estácio SG,Martiniano HF,Faísca PF

更新日期：2016-08-16 00:00:00

abstract：:Structural and functional changes in albumin are of particular interest as numerous studies in vivo have reported a strong involvement of glycated-HSA in the development and progression of chronic diabetic complications. Non-enzymatic addition of glucose molecules to a protein induces structural changes in it. These c...

journal_title：Molecular bioSystems

authors： Neelofar K,Arif Z,Alam K,Ahmad J

更新日期：2016-07-19 00:00:00

abstract：:Production of biofuels has been one of the promising efforts in biotechnology in the past few decades. The perspective of these efforts can be reduction of increasing demands for fossil fuels and consequently reducing environmental pollution. Nonetheless, most previous approaches did not succeed in obviating many big ...

journal_title：Molecular bioSystems

authors： Mohammadi R,Fallah-Mehrabadi J,Bidkhori G,Zahiri J,Javad Niroomand M,Masoudi-Nejad A

更新日期：2016-07-19 00:00:00

abstract：:Human mitogen-activated protein kinase (MAPK) family members JNK and p38 are two homologous protein-serine/threonine kinases but play distinct roles in the pathological process of neurological disorders. Selective targeting of JNK over p38 has been established as a potential therapeutic approach to epilepsy and other ...

journal_title：Molecular bioSystems

authors： Zhuo ZH,Sun YZ,Jin PN,Li FY,Zhang YL,Wang HL

更新日期：2016-07-19 00:00:00

abstract：:The present study describes for the first time, a metabolic profile reflecting the osteoporosis progression in 364 pre- and postmenopausal Chinese women using GC-MS. In order to accurately evaluate the dynamic changes of metabolites along with estrogen deficiency and osteoporosis progression, we divided these subjects...

journal_title：Molecular bioSystems

authors： Qi H,Bao J,An G,Ouyang G,Zhang P,Wang C,Ying H,Ouyang P,Ma B,Zhang Q

更新日期：2016-06-21 00:00:00

abstract：:Hypoxia and inflammation have been identified as the hallmarks of colitis, intertwined with metabolism. Here, we report that halofuginone (HF), an antiparasitic drug, attenuates dextran sulfate sodium (DSS)-induced colitis in mice, as represented by attenuating the disease activity index, inhibiting colonic shortening...

journal_title：Molecular bioSystems

authors： Liu J,Xiao HT,Wang HS,Mu HX,Zhao L,Du J,Yang D,Wang D,Bian ZX,Lin SH

更新日期：2016-06-21 00:00:00