Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.

abstract：:The study of mitochondria and mitochondrial Ca2+ signalling in localised regions is hampered by the lack of tools that can uncouple the mitochondrial membrane potential (DeltaPsi(m)) in a spatially predefined manner. Although there are a number of existing mitochondrial uncouplers, these compounds are necessarily memb...

journal_title：Molecular bioSystems

authors： Avlonitis N,Chalmers S,McDougall C,Stanton-Humphreys MN,Brown CT,McCarron JG,Conway SJ

更新日期：2009-05-01 00:00:00

abstract：:Mimicking receptor flexibility during receptor-ligand binding is a challenging task in computational drug design since it is associated with a large increase in the conformational search space. In the present study, we have devised an in silico design strategy incorporating receptor flexibility in virtual screening to...

journal_title：Molecular bioSystems

authors： Chakraborty S,Ramachandran B,Basu S

更新日期：2014-10-01 00:00:00

abstract：:Insights into the structure and dynamics of modular polyketide synthases (PKS) are essential for understanding the mechanistic details of the biosynthesis of a large number of pharmaceutically important secondary metabolites. The crystal structures of the KS-AT di-domain from erythromycin synthase have revealed the re...

journal_title：Molecular bioSystems

authors： Anand S,Mohanty D

更新日期：2012-04-01 00:00:00

abstract：:The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active doma...

journal_title：Molecular bioSystems

authors： Malik V,Zhang M,Dover LG,Northen JS,Flinn A,Perry JJ,Black GW

更新日期：2013-11-01 00:00:00

abstract：:Ca2+ transport across the sarco/endoplasmic reticulum (SR) plays an essential role in intracellular Ca2+ homeostasis, signalling, cell differentiation and muscle contractility. During SR Ca2+ uptake and release, proton fluxes are required to balance the charge deficit generated by the exchange of Ca2+ and other ions a...

journal_title：Molecular bioSystems

authors： Espinoza-Fonseca LM

更新日期：2017-03-28 00:00:00

abstract：:Peptidylarginine deiminase (PAD) catalyzes the posttranslational citrullination of selected proteins in a calcium dependent manner. The PAD4 isoform has been implicated in multiple sclerosis, rheumatoid arthritis, some types of cancer, and plays a role in gene regulation. However, the substrate selectivity of PAD4 is ...

journal_title：Molecular bioSystems

authors： Guo Q,Bedford MT,Fast W

更新日期：2011-07-01 00:00:00

abstract：:To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO(3)/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence o...

journal_title：Molecular bioSystems

authors： Zhao D,Zhuo RX,Cheng SX

更新日期：2012-03-01 00:00:00

abstract：:The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique am...

journal_title：Molecular bioSystems

authors： Soh N,Seto D,Nakano K,Imato T

更新日期：2006-02-01 00:00:00

abstract：:The ectonucleotide phosphodiesterase/pyrophosphatase-1 (NPP1) is a type II transmembrane glycoprotein that regulates extracellular inorganic purine nucleotide and inorganic diphosphate levels through the hydrolysis of ATP into AMP and diphosphate. NPP1 is a promising drug target as it plays a role in several disorders...

journal_title：Molecular bioSystems

authors： Barbeau X,Mathieu P,Paquin JF,Lagüe P

更新日期：2017-05-30 00:00:00

abstract：:Conversion of the predominantly α-helical cellular prion protein (PrP(C)) to the misfolded β-sheet enriched Scrapie form (PrP(Sc)) is a critical event in prion pathogenesis. However, the conformational triggers that lead to the isoform conversion (PrP(C) to PrP(Sc)) remain obscure, and conjectures about the role of un...

journal_title：Molecular bioSystems

authors： Menon S,Sengupta N

更新日期：2015-05-01 00:00:00

abstract：:Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multiva...

journal_title：Molecular bioSystems

authors： Yu Y,Vidalino L,Anesi A,Macchi P,Guella G

更新日期：2014-04-01 00:00:00

abstract：:The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...

journal_title：Molecular bioSystems

authors： Pirro V,Guffey SC,Sepúlveda MS,Mahapatra CT,Ferreira CR,Jarmusch AK,Cooks RG

更新日期：2016-06-01 00:00:00

abstract：:As a versatile pathogen Staphylococcus aureus can cause various disease patterns, which are influenced by strain specific virulence factor repertoires but also by S. aureus physiological adaptation capacity. Here, we present metabolomic descriptions of S. aureus central metabolic pathways and demonstrate the potential...

journal_title：Molecular bioSystems

authors： Liebeke M,Dörries K,Zühlke D,Bernhardt J,Fuchs S,Pané-Farré J,Engelmann S,Völker U,Bode R,Dandekar T,Lindequist U,Hecker M,Lalk M

更新日期：2011-04-01 00:00:00

abstract：:The recognition and association between the Ca2+/calmodulin-activated protein kinase II-α (CaMKIIα) and the multi-PDZ domain protein 1 (MUPP1) plays an important role in the sperm acrosome reaction and human fertilization. Previously, we have demonstrated that the MUPP1 PDZ11 domain is the primary binding partner of t...

journal_title：Molecular bioSystems

authors： Zhang YL,Han ZF

更新日期：2017-09-26 00:00:00

abstract：:Comparative analysis of the complete genome sequences from a variety of poorly studied organisms aims at predicting ecological and behavioral properties of these organisms and helping in characterizing their habitats. This task requires finding appropriate descriptors that could be correlated with the core traits of e...

journal_title：Molecular bioSystems

authors： Galperin MY,Higdon R,Kolker E

更新日期：2010-04-01 00:00:00

abstract：:Within systems biology there is an increasing interest in the stochastic behavior of genetic and biochemical reaction networks. An appropriate stochastic description is provided by the chemical master equation, which represents a continuous time Markov chain (CTMC). In this paper we consider the stochastic properties ...

journal_title：Molecular bioSystems

authors： Giampieri E,Remondini D,de Oliveira L,Castellani G,Lió P

更新日期：2011-10-01 00:00:00

abstract：:High levels of DNA and RNA released by apoptotic and necrotic cells circulate in the blood of cancer patients. In the present study we determined the applicability of the quantification of nucleic acids and their genetic alterations as minimally invasive tool for breast cancer screening. The relative concentrations of...

journal_title：Molecular bioSystems

authors： Schwarzenbach H,Müller V,Milde-Langosch K,Steinbach B,Pantel K

更新日期：2011-10-01 00:00:00

abstract：:Predicting protein subcellular localization is a challenging problem, particularly when query proteins have multi-label features meaning that they may simultaneously exist at, or move between, two or more different subcellular location sites. Most of the existing methods can only be used to deal with the single-label ...

journal_title：Molecular bioSystems

authors： Lin WZ,Fang JA,Xiao X,Chou KC

更新日期：2013-04-05 00:00:00

abstract：:To identify an agent with specific activity against human lens epithelial cells (HLECs), we confirmed the presence of glucocorticoid receptors (GRs) and GR-α genes and evaluated whether GRs have a relationship with the apoptotic process in cultured HLECs. We also determined whether the inhibitor RU486 could rescue the...

journal_title：Molecular bioSystems

authors： Wang L,Zhao W,Leng F,Ge J,Bu Z,Zhang Y,Liu P

更新日期：2011-06-01 00:00:00

abstract：:Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...

journal_title：Molecular bioSystems

authors： Kang A,Tan MH,Ling H,Chang MW

更新日期：2013-02-02 00:00:00

abstract：:Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...

journal_title：Molecular bioSystems

authors： Yao Z,Yin P,Su D,Peng Z,Zhou L,Ma L,Guo W,Ma L,Xu G,Shi J,Jiao B

更新日期：2011-09-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:Lung fibrosis is a progressive and often fatal lung disease characterized by fibroblast proliferation and excessive deposition of extracellular matrix in the lungs. The chemokine receptor CXCR7 has been shown to control cell adhesion, migration and proliferation by regulating the epithelial-to-mesenchymal transition (...

journal_title：Molecular bioSystems

authors： Guan S,Zhou J

更新日期：2017-09-26 00:00:00

abstract：:The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing sub...

journal_title：Molecular bioSystems

authors： Nakayama S,Sintim HO

更新日期：2010-01-01 00:00:00

abstract：:Proteins are dynamic creatures. Intrinsically disordered proteins (IDPs) function as multiplicity of structures and their activities can only be described by stochastic structure-function relationships. In their complex forms, however, IDPs were thought to lose their plasticity and behave similarly to globular protein...

journal_title：Molecular bioSystems

authors： Fuxreiter M

更新日期：2012-01-01 00:00:00

abstract：:Electrospray ionization mass spectrometry combined with high efficiency capillary liquid chromatography provides high sensitivity and broad dynamic range measurements for the characterization of biological macromolecules in complex matrices, and is an increasingly powerful analytical tool for systems biology research....

journal_title：Molecular bioSystems

authors： Smith RD,Tang K,Shen Y

更新日期：2006-05-01 00:00:00

abstract：:Fluorescent molecules that specifically target amyloid structures are highly desirable for amyloid research. Herein, we show a dimeric design of thioflavin T improves its binding affinity to Abeta amyloid by up to 70 fold, while not sacrificing the specificity and the "light-up" feature upon amyloid binding. ...

journal_title：Molecular bioSystems

authors： Qin L,Vastl J,Gao J

更新日期：2010-10-01 00:00:00

abstract：:Sequence specific binding of DNA and RNA is of fundamental importance in the regulation of cellular gene expression. Because of their modular structure repeat domain proteins are particularly well suited for these processes and have been widely adopted throughout evolution. Detailed biochemical and structural data has...

journal_title：Molecular bioSystems

authors： Filipovska A,Rackham O

更新日期：2012-03-01 00:00:00

abstract：:Transcription factors (TFs) and microRNAs (miRNAs) can jointly regulate transcriptional networks in the form of recurrent circuits or motifs. A motif can be divided into a feedforward loop (FFL) and a feedback loop (FBL). Incoherent FFLs have been the recent focus due to their potential to dampen gene expression noise...

journal_title：Molecular bioSystems

authors： Li Y,Liang C,Easterbrook S,Luo J,Zhang Z

更新日期：2014-12-01 00:00:00

abstract：:In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....

journal_title：Molecular bioSystems

authors： Eltoweissy M,Dihazi GH,Müller GA,Asif AR,Dihazi H

更新日期：2016-05-24 00:00:00