Abstract:
:Fluorescent molecules that specifically target amyloid structures are highly desirable for amyloid research. Herein, we show a dimeric design of thioflavin T improves its binding affinity to Abeta amyloid by up to 70 fold, while not sacrificing the specificity and the "light-up" feature upon amyloid binding.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Qin L,Vastl J,Gao Jdoi
10.1039/c005255hsubject
Has Abstractpub_date
2010-10-01 00:00:00pages
1791-5issue
10eissn
1742-206Xissn
1742-2051journal_volume
6pub_type
杂志文章abstract::As a potential target for the treatment of schizophrenia, the dual cAMP/cGMP hydrolyzing enzyme PDE10A has attracted a significant amount of attention. In the present work, the inhibition mechanism of 116 structurally diverse quinoline derivatives as PDE10A inhibitors was explored by 3D-QSAR, molecular docking and mol...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25501d
更新日期:2013-03-01 00:00:00
abstract::Naphthalene, a polycyclic aromatic hydrocarbon, is a ubiquitous environmental pollutant capable of causing illness. In this study, we deconvoluted the metabolites related to naphthalene intervention in various organs by using nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00090k
更新日期:2014-07-01 00:00:00
abstract::The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potent...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b822268c
更新日期:2009-07-01 00:00:00
abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::Diabetic retinopathy (DR) is a serious microvascular syndrome of diabetes, and is one of the most frequent causes of blindness in the world. It has three progressive stages with complex metabolic deregulations in the holistic system of Western medicine. Chinese medicine classifies DR into two different syndrome types;...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00341g
更新日期:2011-07-01 00:00:00
abstract::Remineralization of organic matter in deep-sea sediments is important in oceanic biogeochemical cycles, and bacteria play a major role in this process. Shewanella violacea DSS12 is a psychrophilic and piezophilic gamma-proteobacterium that was isolated from the surface layer of deep sea sediment at a depth of 5110 m. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c000396d
更新日期:2010-07-01 00:00:00
abstract::New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b914822c
更新日期:2010-02-01 00:00:00
abstract::When exposed to low concentrations of toxic chemicals, bacteria modulate the expression of a number of cellular processes. Typically, these processes include those related to porin production, dismutases, and metabolic fluxes. In Escherichia coli (E. coli), the expression of these systems is largely controlled by thre...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00263c
更新日期:2016-05-24 00:00:00
abstract::RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe t...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b603229j
更新日期:2006-06-01 00:00:00
abstract::Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multiva...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70540d
更新日期:2014-04-01 00:00:00
abstract::The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active doma...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70303g
更新日期:2013-11-01 00:00:00
abstract::Electrospray ionization mass spectrometry combined with high efficiency capillary liquid chromatography provides high sensitivity and broad dynamic range measurements for the characterization of biological macromolecules in complex matrices, and is an increasingly powerful analytical tool for systems biology research....
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b601468b
更新日期:2006-05-01 00:00:00
abstract::Regular physical activity is associated with a reduced risk of coronary heart disease, as it probably modifies the balance between free-radical generation and antioxidant activity. On the other hand, however, acute physical activity increases oxygen uptake and leads to a temporary imbalance between the production of r...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00106f
更新日期:2011-03-01 00:00:00
abstract::The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00168h
更新日期:2016-06-01 00:00:00
abstract::Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05029j
更新日期:2011-09-01 00:00:00
abstract::The study of mitochondria and mitochondrial Ca2+ signalling in localised regions is hampered by the lack of tools that can uncouple the mitochondrial membrane potential (DeltaPsi(m)) in a spatially predefined manner. Although there are a number of existing mitochondrial uncouplers, these compounds are necessarily memb...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b820415m
更新日期:2009-05-01 00:00:00
abstract::To understand the regulatory dynamics of transcription factors (TFs) and their interplay with other cellular components we have integrated transcriptional, protein-protein and the allosteric or equivalent interactions which mediate the physiological activity of TFs in Escherichia coli. To study this integrated network...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25279a
更新日期:2012-11-01 00:00:00
abstract::The glutamate γ-carboxylation plays a pivotal part in a number of important human diseases. However, traditional protein γ-carboxylation site detection by experimental approaches are often laborious and time-consuming. In this study, we initiated an attempt for the computational prediction of protein γ-carboxylation s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25185j
更新日期:2012-11-01 00:00:00
abstract::Protein-protein interactions play an essential role in the regulation of various cellular processes. Understanding the recognition mechanism of protein-protein complexes is a challenging task in molecular and computational biology. In this work, we have developed an energy based approach for identifying the binding si...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/B904161N
更新日期:2009-12-01 00:00:00
abstract::The size and complexity of cellular systems make building predictive models an extremely difficult task. In principle dynamical time-course data can be used to elucidate the structure of the underlying molecular mechanisms, but a central and recurring problem is that many and very different models can be fitted to exp...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00107d
更新日期:2011-05-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor participating in numerous redox reactions. It is also a substrate for regulatory modifications of proteins and nucleic acids via the addition of ADP-ribose moieties or removal of acyl groups by transfer to ADP-ribose. In this study, we use in-depth seque...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05487f
更新日期:2012-06-01 00:00:00
abstract::The importance of microRNA (miRNA) in modulating gene expression at the post-transcriptional level is well known. Such regulation has been shown to influence the dynamics of several regulatory networks including the cell cycle. In this study we incorporated regulatory effects of intronic miRNA into an existing mathema...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25046b
更新日期:2012-08-01 00:00:00
abstract::The small molecule 4-hydroxy-3-methoxybenzaldehyde (5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-4-yl)hydrazone (Exo2) stimulates morphological changes at the mammalian Golgi and trans-Golgi network that are virtually indistinguishable from those induced by brefeldin A. Both brefeldin A and Exo2 protect cells from...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00035c
更新日期:2010-10-01 00:00:00
abstract::Ebola virus (EBOV) is highly lethal due to virally encoded immune antagonists, and the combination of EBOV VP24 with karyopherin alpha (KPNA) will trigger anti-interferon (IFN) signaling. The crystal structure of VP24-KPNA5 has been proposed in recent studies, but the precise binding mechanisms are still unclear. In o...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00136c
更新日期:2017-05-02 00:00:00
abstract::Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25259g
更新日期:2013-02-02 00:00:00
abstract::Lovastatin and other statins inhibit HMG-CoA reductase, which carries out an early step in the sterol biosynthesis pathway. Statins lower cholesterol and are widely prescribed to prevent heart disease, but like many drugs, they can interact with nutritionally acquired metabolites. To probe these interactions, we explo...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00166j
更新日期:2011-02-01 00:00:00
abstract::We demonstrate the expected preference of an immobilised oligosaccharide Man(9)(GlcNAc)(2) upon a 96-well photochemical array, for its known receptor, the cell-surface lectin Dendritic Cell-Specific ICAM3 Grabbing Nonintegrin (DC-SIGN) when compared to immobilised competing monosaccharides. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00118j
更新日期:2011-01-01 00:00:00
abstract::Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for recombinant proteins. However, in vitro methods for Drosophila cell and organ c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00155a
更新日期:2014-10-01 00:00:00
abstract::Normalization of high-throughput molecular expression profiles secures differential expression analysis between samples of different phenotypes or biological conditions, and facilitates comparison between experimental batches. While the same general principles apply to microRNA (miRNA) normalization, there is mounting...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00711e
更新日期:2015-05-01 00:00:00
abstract::Calcium (Ca(2+)) is used as a signaling molecule to regulate many cellular processes. Calcium signaling generally involves transient elevations of the concentration of free Ca(2+) in the cytosol. More pronounced and sustained elevations of intracellular Ca(2+) concentrations are observed during apoptosis (programmed c...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b920805d
更新日期:2010-05-01 00:00:00