Abstract:
:The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-throughput gene synthesis technology. The capability of generating unlimited supplies of DNA molecules of any sequence or size will transform biomedical research in the near future.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Tian J,Ma K,Saaem Idoi
10.1039/b822268csubject
Has Abstractpub_date
2009-07-01 00:00:00pages
714-22issue
7eissn
1742-206Xissn
1742-2051journal_volume
5pub_type
杂志文章,评审abstract::In vivo analysis is of paramount importance in monitoring physiological processes that take place in living organisms. Mass spectrometry, an analytical technique with high speed, sensitivity and specificity, is indispensable in biochemical studies nowadays. However, traditional mass spectrometric techniques are of lim...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25428j
更新日期:2013-05-01 00:00:00
abstract::As one of the most important trace elements within an organism, zinc has been shown to be involved in numerous biological processes and closely implicated in various diseases. The zinc ion is important for proteins to perform their functional roles. To provide in-depth functional annotation of zinc-binding proteins, a...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70100j
更新日期:2013-09-01 00:00:00
abstract::The biosynthesis of the endocannabinoid anandamide (AEA) and related N-acyl ethanolamine (NAE) lipids is complex and appears to involve multiple pathways, including: (1) direct release of NAEs from N-acyl phosphatidyl ethanolamine (NAPE) precursors by the phosphodiesterase NAPE-PLD, and (2) double O-deacylation of NAP...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c000237b
更新日期:2010-08-01 00:00:00
abstract::New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b914822c
更新日期:2010-02-01 00:00:00
abstract::Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05029j
更新日期:2011-09-01 00:00:00
abstract::The neuroscientific community rapidly adopted RNA interference techniques as an experimental tool for the dissection of gene function in vitro and in animal models of neurological disease in vivo. Here, we discuss recent advances in the biotechnical implementation of siRNA/shRNA-mediated gene silencing focusing on iss...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b701169e
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:Candida albicans, the most common fungal pathogen related to colonization and biofilm formation on the surfaces of indwelling medical devices, shows high resistance to the most commonly used antifungal drugs. In this study, an ion-pairing chromatography-porous graphitic carbon column coupled with a time-of-f...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70240e
更新日期:2014-01-01 00:00:00
abstract::The effect of the glutathione transferase P1-1 (GSTP1-1) targeting has been investigated in both sensitive (U-2OS) and cisplatin-resistant (U-2OS/CDDP4 μg) human osteosarcoma cell lines. Despite the different enzyme's content, inhibition of GSTP1-1 by 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX) causes the...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05295k
更新日期:2012-04-01 00:00:00
abstract::In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00887e
更新日期:2016-05-24 00:00:00
abstract::Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur, which compromise the experimental results. In...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00393e
更新日期:2017-11-21 00:00:00
abstract::In the field of synthetic biology, recent genetic engineering efforts have enabled the construction of novel genetic circuits with diverse functionalities and unique activation mechanisms. Because of these advances, artificial genetic networks are becoming increasingly complex, and are demonstrating more robust behavi...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b700547d
更新日期:2007-12-01 00:00:00
abstract::The eIF4E-binding protein 1 (4EBP1) has long been known to be completely unstructured without any secondary structures, which contributed significantly to the proposal of the induced fit mechanism for target binding of intrinsically disordered proteins. We show here that 4EBP1 is not completely unstructured, but conta...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00532e
更新日期:2015-02-01 00:00:00
abstract::The effect of aging and development in male Wistar-derived rats on the profile of endogenous metabolites excreted in the urine was investigated using both (1)H NMR spectroscopy and HPLC-TOF MS using electrospray ionisation (ESI). The endogenous metabolites were profiled in samples collected from male rats every two we...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b500852b
更新日期:2005-07-01 00:00:00
abstract::Recently, accumulating studies have indicated that microRNAs (miRNAs) play an important role in exploring the pathogenesis of various human diseases at the molecular level and may result in the design of specific tools for diagnosis, treatment evaluation and prevention. Experimental identification of disease-related m...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00049e
更新日期:2016-06-21 00:00:00
abstract::Genetic information in genomes is ordered, arranged in such a way that it constitutes a code, the so-called cis regulatory code. The regulatory machinery of the cell, termed trans-factors, decodes and expresses this information. In this way, genomes maintain a potential repertoire of genetic programs, parts of which a...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c6mb00789a
更新日期:2017-03-28 00:00:00
abstract::The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70616h
更新日期:2014-05-01 00:00:00
abstract::Genome sequencing technology has generated a vast amount of genomic and epigenomic data, and has provided us a great opportunity to study gene functions on a global scale from an epigenomic view. In the last decade, network-based studies, such as those based on PPI networks and co-expression networks, have shown good ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00430c
更新日期:2017-10-24 00:00:00
abstract::Synthetic studies of lipid A and LPS partial structures have been performed to investigate the relationship between structures and functions of LPS. Recent studies have suggested several pathological implications of LPS from parasitic bacteria due to its influence on the host immune responses. To address this issue, w...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25477a
更新日期:2013-05-01 00:00:00
abstract::Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00529e
更新日期:2015-02-01 00:00:00
abstract::Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00268k
更新日期:2015-09-01 00:00:00
abstract::A rapid and sensitive gold-nanobioprobe based immunoassay format has been presented for the detection of capsular Vi polysaccharide of Salmonella enterica serovar Typhi (surface antigen) using anti-Vi antibodies. The Vi antigen was extracted from serovar Typhi cells, under the optimised growth conditions for its over-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25048a
更新日期:2012-07-06 00:00:00
abstract::Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsic...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25135c
更新日期:2012-10-01 00:00:00
abstract::The human corticotropin-releasing factor receptor type 1 (CRF1R) is a class B G-protein-coupled receptor (GPCR), which mediates the response to stress and has been considered as a drug target for depression and anxiety. Based on the CRF1R-antagonist crystal structure, we study the binding mechanism of two distinct ant...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00159e
更新日期:2015-07-01 00:00:00
abstract::Bacterial biosynthesis of lysine has come under increased scrutiny as a target for novel antibacterial agents as it provides lysine for protein synthesis and both lysine and meso-diaminopimelate for construction of the bacterial peptidoglycan cell wall. In this Highlight article we review recent advances in the valida...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b705624a
更新日期:2007-07-01 00:00:00
abstract::Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b801245h
更新日期:2008-07-01 00:00:00
abstract::The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00747j
更新日期:2016-02-01 00:00:00
abstract::Normalization of high-throughput molecular expression profiles secures differential expression analysis between samples of different phenotypes or biological conditions, and facilitates comparison between experimental batches. While the same general principles apply to microRNA (miRNA) normalization, there is mounting...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00711e
更新日期:2015-05-01 00:00:00
abstract::Production of biofuels has been one of the promising efforts in biotechnology in the past few decades. The perspective of these efforts can be reduction of increasing demands for fossil fuels and consequently reducing environmental pollution. Nonetheless, most previous approaches did not succeed in obviating many big ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00119j
更新日期:2016-07-19 00:00:00
abstract::Deciphering functional interactions between proteins is one of the great challenges in biology. Sequence-based homology-free encoding schemes have been increasingly applied to develop promising protein-protein interaction (PPI) predictors by means of statistical or machine learning methods. Here we analyze the relatio...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05427b
更新日期:2012-04-01 00:00:00
abstract::Biological networks are effective tools for studying molecular interactions. Modular structure, in which genes or proteins may tend to be associated with functional modules or protein complexes, is a remarkable feature of biological networks. Mining modular structure from biological networks enables us to focus on a s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00617e
更新日期:2016-11-15 00:00:00