Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics.

abstract：:The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potent...

journal_title：Molecular bioSystems

authors： Tian J,Ma K,Saaem I

更新日期：2009-07-01 00:00:00

abstract：:The recognition and association between the Ca2+/calmodulin-activated protein kinase II-α (CaMKIIα) and the multi-PDZ domain protein 1 (MUPP1) plays an important role in the sperm acrosome reaction and human fertilization. Previously, we have demonstrated that the MUPP1 PDZ11 domain is the primary binding partner of t...

journal_title：Molecular bioSystems

authors： Zhang YL,Han ZF

更新日期：2017-09-26 00:00:00

abstract：:Insights into the structure and dynamics of modular polyketide synthases (PKS) are essential for understanding the mechanistic details of the biosynthesis of a large number of pharmaceutically important secondary metabolites. The crystal structures of the KS-AT di-domain from erythromycin synthase have revealed the re...

journal_title：Molecular bioSystems

authors： Anand S,Mohanty D

更新日期：2012-04-01 00:00:00

abstract：:Gene regulatory networks constitute the first layer of the cellular computation for cell adaptation and surveillance. In these webs, a set of causal relations is built up from thousands of interactions between transcription factors and their target genes. The large size of these webs and their entangled nature make it...

journal_title：Molecular bioSystems

authors： Rodríguez-Caso C,Corominas-Murtra B,Solé RV

更新日期：2009-12-01 00:00:00

abstract：:It is now widely accepted that microRNAs (miRNAs or miRs) along with transcription factors (TFs) weave a complex inter-regulatory network within the cell that is responsible for the combinatorial regulation of gene expression. Recently we have shown that miRNAs and TFs that form network clusters are also associated wi...

journal_title：Molecular bioSystems

authors： Sengupta D,Bandyopadhyay S

更新日期：2013-06-01 00:00:00

abstract：:Diabetic nephropathy (DN) is a serious metabolic disease, and comprehensive understanding of its complex mechanism will help in preventing the onset and progression of DN. To reveal the systemic metabolic changes associated with renal injury, we performed 1H NMR-based metabonomic and multivariate analyses to analyze s...

journal_title：Molecular bioSystems

authors： Liu J,Wang D,Chen Y,Sun H,He S,Wang C,Yang G,Shi M,Zhang J,Ren Y,Wang L,Lu Y,Cheng J

更新日期：2013-11-01 00:00:00

abstract：:The glutamate γ-carboxylation plays a pivotal part in a number of important human diseases. However, traditional protein γ-carboxylation site detection by experimental approaches are often laborious and time-consuming. In this study, we initiated an attempt for the computational prediction of protein γ-carboxylation s...

journal_title：Molecular bioSystems

authors： Zhang N,Li BQ,Gao S,Ruan JS,Cai YD

更新日期：2012-11-01 00:00:00

abstract：:Molecular docking methods were applied to simulate the coupling of a set of nineteen acyl homoserine lactone analogs into the binding site of the transcriptional receptor LasR. The best pose of each ligand was explored and a qualitative analysis of the possible interactions present in the complex was performed. From t...

journal_title：Molecular bioSystems

authors： Ahumedo M,Drosos JC,Vivas-Reyes R

更新日期：2014-05-01 00:00:00

abstract：:The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel. ...

journal_title：Molecular bioSystems

authors： Kim J,Kwon D,Lee J,Pasquier H,Grailhe R

更新日期：2009-02-01 00:00:00

abstract：:Large combinatorial libraries of macrocyclic peptides are a useful source of bioactive compounds. However, peptides are not generally cell permeable, so there is great interest in the development of methods to create large libraries of modified peptides. In particular, N-alkylation of peptides is known to improve thei...

journal_title：Molecular bioSystems

authors： Morimoto J,Kodadek T

更新日期：2015-10-01 00:00:00

abstract：:Information on extracellular signals and conditions is often transduced by biological systems using cascades of protein phosphorylation that affect the activity of enzymes, the localization of proteins and gene expression. A model to study signal transduction is the response of the yeast Saccharomyces cerevisiae to os...

journal_title：Molecular bioSystems

authors： Soufi B,Kelstrup CD,Stoehr G,Fröhlich F,Walther TC,Olsen JV

更新日期：2009-11-01 00:00:00

abstract：:4-Hydroxyderricin is a heat labile bioactive chalcone isolated from the plant Angelica keiskei. It received attention due to its antibiotic potency against several strains of bacteria including pathogens such as Staphylococcus aureus. Despite these promising pharmacological properties, the exact mode of action or the ...

journal_title：Molecular bioSystems

authors： Battenberg OA,Yang Y,Verhelst SH,Sieber SA

更新日期：2013-03-01 00:00:00

abstract：:It is well known that oligomeric/aggregated amyloid β peptides are a key player in the pathogenesis of Alzheimer's disease and that different nanoparticles influence oligomerization/aggregation processes in experiments in vitro. Our previous results demonstrated antiaggregation effects of magnetite nanoparticles in th...

journal_title：Molecular bioSystems

authors： Gažová Z,Antošová A,Krištofiková Z,Bartoš A,Ríčný J,Cechová L,Klaschka J,Rípová D

更新日期：2010-11-01 00:00:00

abstract：:Protein-protein interactions are important for the majority of biological processes. A significant number of computational methods have been developed to predict protein-protein interactions using protein sequence, structural and genomic data. Vast experimental data is publicly available on the Internet, but it is sca...

journal_title：Molecular bioSystems

authors： Saha I,Zubek J,Klingström T,Forsberg S,Wikander J,Kierczak M,Maulik U,Plewczynski D

更新日期：2014-04-01 00:00:00

abstract：:There are clearly many different philosophies associated with adapting fragment screening into mainstream Drug Discovery Lead Generation strategies. Scientists at Astex, for instance, focus entirely on strategies involving use of X-ray crystallography and NMR. However, AstraZeneca uses a number of different fragment s...

journal_title：Molecular bioSystems

authors： Leach AR,Hann MM,Burrows JN,Griffen EJ

更新日期：2006-09-01 00:00:00

abstract：:Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...

journal_title：Molecular bioSystems

authors： Pan X,Xiong K

更新日期：2015-08-01 00:00:00

abstract：:In the last few decades a negative association between the level of milk production and fertility has been observed. Currently, the most utilized method of measuring male fertility employed by the livestock industry is related to the Non-Return Rate (NRR). Through differential proteome analysis, this study evaluated c...

journal_title：Molecular bioSystems

authors： Soggiu A,Piras C,Hussein HA,De Canio M,Gaviraghi A,Galli A,Urbani A,Bonizzi L,Roncada P

更新日期：2013-06-01 00:00:00

abstract：:New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...

journal_title：Molecular bioSystems

authors： Greco SJ,Rameshwar P

更新日期：2010-02-01 00:00:00

abstract：:Cells of the bacterium Myxococcus xanthus organize into two types of patterns depending on their nutritional status, i.e. in the presence of nutrients cells form spreading colonies and in the absence of nutrients cells form fruiting bodies. Formation of both patterns depends on directed cell movements, which, in turn,...

journal_title：Molecular bioSystems

authors： Leonardy S,Bulyha I,Søgaard-Andersen L

更新日期：2008-10-01 00:00:00

abstract：:MicroRNAs are a predominant type of small non-coding RNAs approximately 21 nucleotides in length that play an essential role at the post-transcriptional level by either RNA degradation, translational repression or both through an RNA-induced silencing complex. Identification of these molecules can aid the dissecting o...

journal_title：Molecular bioSystems

authors： Yao Y,Ma C,Deng H,Liu Q,Zhang J,Yi M

更新日期：2016-10-20 00:00:00

abstract：:Bacterial biosynthesis of lysine has come under increased scrutiny as a target for novel antibacterial agents as it provides lysine for protein synthesis and both lysine and meso-diaminopimelate for construction of the bacterial peptidoglycan cell wall. In this Highlight article we review recent advances in the valida...

journal_title：Molecular bioSystems

authors： Hutton CA,Perugini MA,Gerrard JA

更新日期：2007-07-01 00:00:00

abstract：:Many Gram-negative bacterial pathogens use type III secretion systems (TTSSs) for subverting the normal cellular functions of their target eukaryotic cells. The type III secretion apparatus (TTSA) functions like a syringe to inject proteins through an external needle and into a target cell's membrane and cytosol. The ...

journal_title：Molecular bioSystems

authors： Wang Y,Zhang L,Picking WL,Picking WD,De Guzman RN

更新日期：2008-12-01 00:00:00

abstract：:Ca2+ transport across the sarco/endoplasmic reticulum (SR) plays an essential role in intracellular Ca2+ homeostasis, signalling, cell differentiation and muscle contractility. During SR Ca2+ uptake and release, proton fluxes are required to balance the charge deficit generated by the exchange of Ca2+ and other ions a...

journal_title：Molecular bioSystems

authors： Espinoza-Fonseca LM

更新日期：2017-03-28 00:00:00

abstract：:Genetic information in genomes is ordered, arranged in such a way that it constitutes a code, the so-called cis regulatory code. The regulatory machinery of the cell, termed trans-factors, decodes and expresses this information. In this way, genomes maintain a potential repertoire of genetic programs, parts of which a...

journal_title：Molecular bioSystems

authors： Rangel-Chavez C,Galan-Vasquez E,Martinez-Antonio A

更新日期：2017-03-28 00:00:00

abstract：:Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...

journal_title：Molecular bioSystems

authors： Li S,Wang Y,Zhang J,Yang WH,Dai ZH,Zhu W,Yu XQ

更新日期：2011-04-01 00:00:00

abstract：:Epithelial to mesenchymal transition (EMT) is a biological process that plays a crucial role in cancer metastasis. Although studies regarding the EMT mechanisms are usual in terms of gene expression and protein functions, little is known about the involvement of lipids in EMT. In this work, an untargeted lipidomic ana...

journal_title：Molecular bioSystems

authors： Dalmau N,Jaumot J,Tauler R,Bedia C

更新日期：2015-12-01 00:00:00

abstract：:Protein tyrosine phosphatases are crucial regulators of signal transduction and function as antagonists towards protein tyrosine kinases to control reversible tyrosine phosphorylation, thereby regulating fundamental physiological processes. Growing evidence has supported the notion that reversible oxidative inactivati...

journal_title：Molecular bioSystems

authors： Garcia FJ,Carroll KS

更新日期：2016-05-24 00:00:00

abstract：:Schistosomiasis is a parasitic infection that is endemic in many developing countries in the tropics and subtropics afflicting more than 207 million people primarily in rural areas. After malaria, it is the second most important parasitic infection in terms of socio-economic and public health. Investigation of the hos...

journal_title：Molecular bioSystems

authors： Balog CI,Meissner A,Göraler S,Bladergroen MR,Vennervald BJ,Mayboroda OA,Deelder AM

更新日期：2011-05-01 00:00:00

abstract：:The ectonucleotide phosphodiesterase/pyrophosphatase-1 (NPP1) is a type II transmembrane glycoprotein that regulates extracellular inorganic purine nucleotide and inorganic diphosphate levels through the hydrolysis of ATP into AMP and diphosphate. NPP1 is a promising drug target as it plays a role in several disorders...

journal_title：Molecular bioSystems

authors： Barbeau X,Mathieu P,Paquin JF,Lagüe P

更新日期：2017-05-30 00:00:00

abstract：:Precise quantification of endogenous protein-protein interactions across live cells would be a major boon to biology. Such precise measurement is theoretically possible with fluorescence lifetime imaging microscopy (FLIM) but requires first properly addressing multiple biological, instrumental, statistical, and photop...

journal_title：Molecular bioSystems

authors： Walther KA,Papke B,Sinn MB,Michel K,Kinkhabwala A

更新日期：2011-02-01 00:00:00