The use of Cyan Fluorescent Protein variants with a distinctive lifetime signature.

abstract：:α-Oxoaldehydes, which are produced in higher quantities in diabetes, uremia, oxidative stress, inflammation and aging, react with the amino, guanidine and thiol groups of proteins and cause the formation of advanced glycated end-products and protein cross-linking. To prevent these reactions, the efficiency of low mole...

journal_title：Molecular bioSystems

authors： Aćimović JM,Penezić AZ,Pavićević ID,Jovanović VB,Mandić LM

更新日期：2014-08-01 00:00:00

abstract：:Inferring metabolic networks from metabolite concentration data is a central topic in systems biology. Mathematical techniques to extract information about the network from data have been proposed in the literature. This paper presents a critical assessment of the feasibility of reverse engineering of metabolic networ...

journal_title：Molecular bioSystems

authors： Hendrickx DM,Hendriks MM,Eilers PH,Smilde AK,Hoefsloot HC

更新日期：2011-02-01 00:00:00

abstract：:This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target...

journal_title：Molecular bioSystems

authors： Jeong HS,Choi SM,Kim HW,Park JW,Park HN,Park SM,Jang SK,Rhee YM,Kim BH

更新日期：2013-05-01 00:00:00

abstract：:Cellular heterogeneity and stochastic fluctuation play key roles in biological processes. Single molecule approaches have the key advantage of avoiding ensemble averaging, allowing the observation of transient intermediates and heterogeneity (both static and dynamic). Thus they have revolutionised the way many biologi...

journal_title：Molecular bioSystems

authors： Ying L

更新日期：2007-06-01 00:00:00

abstract：:The Library of Integrated Network-based Cellular Signatures (LINCS) L1000 big data provide gene expression profiles induced by over 10 000 compounds, shRNAs, and kinase inhibitors using the L1000 platform. We developed csNMF, a systematic compound signature discovery pipeline covering from raw L1000 data processing to...

journal_title：Molecular bioSystems

authors： Liu C,Su J,Yang F,Wei K,Ma J,Zhou X

更新日期：2015-03-01 00:00:00

abstract：:The human corticotropin-releasing factor receptor type 1 (CRF1R) is a class B G-protein-coupled receptor (GPCR), which mediates the response to stress and has been considered as a drug target for depression and anxiety. Based on the CRF1R-antagonist crystal structure, we study the binding mechanism of two distinct ant...

journal_title：Molecular bioSystems

authors： Xu J,Wang Z,Liu P,Li D,Lin J

更新日期：2015-07-01 00:00:00

abstract：:The identification of genetic markers (e.g. genes, pathways and subnetworks) for cancer has been one of the most challenging research areas in recent years. A subset of these studies attempt to analyze genome-wide expression profiles to identify markers with high reliability and reusability across independent whole-tr...

journal_title：Molecular bioSystems

authors： Farahmand S,Goliaei S,Ansari-Pour N,Razaghi-Moghadam Z

更新日期：2016-03-01 00:00:00

abstract：:Mirror image phage display is a straightforward approach to identify new potentially therapeutically active D-enantiomeric peptides. Such D-peptides are more resistant to proteolytic degradation compared to L-peptides. In this review, several examples of mirror image phage display derived D-peptides with therapeutical...

journal_title：Molecular bioSystems

authors： Funke SA,Willbold D

更新日期：2009-08-01 00:00:00

abstract：:Extracellular electron transfer (EET) is the key feature of some bacteria, such as Geobacter sulfurreducens and Shewanella oneidensis. Via EET processes, these bacteria can grow on electrode surfaces and make current output of microbial fuel cells. c-Type cytochromes can be used as carriers to transfer electrons, whic...

journal_title：Molecular bioSystems

authors： Ding DW,Xu J,Li L,Xie JM,Sun X

更新日期：2014-12-01 00:00:00

abstract：:Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...

journal_title：Molecular bioSystems

authors： Kumar KM,Anbarasu A,Ramaiah S

更新日期：2014-04-01 00:00:00

abstract：:Upon rapid ascent to a high altitude, non-acclimatized individuals, although healthy, are highly prone to contracting high-altitude pulmonary edema (HAPE). Early diagnosis is difficult and there is no reliable biomarker available. We used proton ((1)H) NMR metabolomics to profile the altered metabolic patterns of bloo...

journal_title：Molecular bioSystems

authors： Luo Y,Zhu J,Gao Y

更新日期：2012-06-01 00:00:00

abstract：:Fabry disease (FD) is an X-linked lysosomal storage disorder caused by a deficiency of the lysosomal hydrolase α-galactosidase A (α-GalA) that leads to the intra-lysosomal accumulation of globotriaosylceramide (Gb3) in various organ systems. As a consequence, a multisystems disorder develops, culminating in stroke, pr...

journal_title：Molecular bioSystems

authors： Matafora V,Cuccurullo M,Beneduci A,Petrazzuolo O,Simeone A,Anastasio P,Mignani R,Feriozzi S,Pisani A,Comotti C,Bachi A,Capasso G

更新日期：2015-06-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:Small molecule modulators are critical for dissecting and understanding signaling pathways at the molecular level. Interleukin 6 (IL-6) is a cytokine that signals via the JAK-STAT pathway and is implicated in cancer and inflammation. To identify modulators of this pathway, we screened a chemical collection against an ...

journal_title：Molecular bioSystems

authors： Johnson RL,Huang R,Jadhav A,Southall N,Wichterman J,MacArthur R,Xia M,Bi K,Printen J,Austin CP,Inglese J

更新日期：2009-09-01 00:00:00

abstract：:Cell culture metabolomics has demonstrated significant advantages in cancer research. However, its applications have been impeded by some influencing factors such as culture media, which could significantly affect cellular metabolic profiles and lead to inaccuracy and unreliability of comparative metabolomic analysis ...

journal_title：Molecular bioSystems

authors： Huang Z,Shao W,Gu J,Hu X,Shi Y,Xu W,Huang C,Lin D

更新日期：2015-07-01 00:00:00

abstract：:The essential idea of regenerative medicine is to fix or replace tissues or organs with alive and patient-specific implants. Pluripotent stem cells are able to indefinitely self-renew and differentiate into all cell types of the body which makes them a potent substantial player in regenerative medicine. The easily acc...

journal_title：Molecular bioSystems

authors： Baranek M,Belter A,Naskręt-Barciszewska MZ,Stobiecki M,Markiewicz WT,Barciszewski J

更新日期：2017-01-31 00:00:00

abstract：:Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...

journal_title：Molecular bioSystems

authors： Lowther J,Yard BA,Johnson KA,Carter LG,Bhat VT,Raman MC,Clarke DJ,Ramakers B,McMahon SA,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Affinity probes are useful tools for determining molecular targets and elucidating mechanism of action for novel, bioactive compounds. In the case of covalent inhibitors, activity based probes are particularly valuable for ensuring acceptable selectivity margins. However, there is a variety of bioorthogonal chemistry ...

journal_title：Molecular bioSystems

authors： Paresi CJ,Liu Q,Li YM

更新日期：2016-05-01 00:00:00

abstract：:Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...

journal_title：Molecular bioSystems

authors： Kang A,Tan MH,Ling H,Chang MW

更新日期：2013-02-02 00:00:00

abstract：:As one of the most important trace elements within an organism, zinc has been shown to be involved in numerous biological processes and closely implicated in various diseases. The zinc ion is important for proteins to perform their functional roles. To provide in-depth functional annotation of zinc-binding proteins, a...

journal_title：Molecular bioSystems

authors： Chen Z,Wang Y,Zhai YF,Song J,Zhang Z

更新日期：2013-09-01 00:00:00

abstract：:A novel class of pH-sensitive PEG lipids bearing acid-cleavable acetal linkages and short PEG chains have been synthesised and used in ternary vector formulations. The cleavage pH was influenced by structural components including the terminal PEG moiety and spacer length. ...

journal_title：Molecular bioSystems

authors： Wong JB,Grosse S,Tabor AB,Hart SL,Hailes HC

更新日期：2008-06-01 00:00:00

abstract：:Non-ribosomal peptide synthetases (NRPSs) are enzymes that catalyze ribosome-independent production of small peptides, most of which are bioactive. NRPSs act as peptide assembly lines where individual, often interconnected modules each incorporate a specific amino acid into the nascent chain. The modules themselves co...

journal_title：Molecular bioSystems

authors： Beer R,Herbst K,Ignatiadis N,Kats I,Adlung L,Meyer H,Niopek D,Christiansen T,Georgi F,Kurzawa N,Meichsner J,Rabe S,Riedel A,Sachs J,Schessner J,Schmidt F,Walch P,Niopek K,Heinemann T,Eils R,Di Ventura B

更新日期：2014-07-01 00:00:00

abstract：:The adaptation of the cellular network to functional changes, timing and patterning of gene expression is regulated by binding of transcription factors to gene regulatory elements, which in turn depends on co-occurring histone modifications. These two layers influence each other, enabling a further level of regulatory...

journal_title：Molecular bioSystems

authors： Schueler M,Zhang Q,Schlesinger J,Tönjes M,Sperling SR

更新日期：2012-02-01 00:00:00

abstract：:The major mechanical function of talin is to couple the β-integrin cytoplasmic tails to actin filaments. A variety of β-integrin tails contain conserved binding motifs for talin, and recent research shows that β-integrins differ both in affinity to talin and preferences for other cytoplasmic adaptor proteins. While ta...

journal_title：Molecular bioSystems

authors： Kukkurainen S,Määttä JA,Saeger J,Valjakka J,Vogel V,Hytönen VP

更新日期：2014-12-01 00:00:00

abstract：:A major effort in systems biology is the development of mathematical models that describe complex biological systems at multiple scales and levels of abstraction. Determining the topology-the set of interactions-of a biological system from observations of the system's behavior is an important and difficult problem. He...

journal_title：Molecular bioSystems

authors： Hagen DR,Tidor B

更新日期：2015-02-01 00:00:00

abstract：:New-type oseltamivir-resistant H1N1 influenza viruses have been a major threat to human health since the 2009 flu pandemic. To resolve the drug resistance issue, we aimed to identify a new type of inhibitors against H1 from traditional Chinese medicine (TCM) by employing the world's largest TCM database () for virtual...

journal_title：Molecular bioSystems

authors： Chang SS,Huang HJ,Chen CY

更新日期：2011-12-01 00:00:00

abstract：:The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active doma...

journal_title：Molecular bioSystems

authors： Malik V,Zhang M,Dover LG,Northen JS,Flinn A,Perry JJ,Black GW

更新日期：2013-11-01 00:00:00

abstract：:DNA-binding proteins play a pivotal role in various biological activities. Identification of DNA-binding residues (DBRs) is of great importance for understanding the mechanism of gene regulations and chromatin remodeling. Most traditional computational methods usually construct their predictors on static non-redundant...

journal_title：Molecular bioSystems

authors： Chai H,Zhang J,Yang G,Ma Z

更新日期：2016-11-15 00:00:00

abstract：:Proteomics is the study of proteins and their interactions within complex biological systems. While this field is often associated with mass spectrometry, it is more useful to consider proteomics in the context of an objective: to identify and understand the molecular basis of health and disease at the protein level i...

journal_title：Molecular bioSystems

authors： Bark SJ,Hook V

更新日期：2007-01-01 00:00:00

abstract：:Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies d...

journal_title：Molecular bioSystems

authors： Ven Chang I,Tsutsumi H,Mihara H

更新日期：2017-10-24 00:00:00