Abstract:
:α-Oxoaldehydes, which are produced in higher quantities in diabetes, uremia, oxidative stress, inflammation and aging, react with the amino, guanidine and thiol groups of proteins and cause the formation of advanced glycated end-products and protein cross-linking. To prevent these reactions, the efficiency of low molecular mass thiols with an α-amino-β-mercapto-ethane group (Cys, penicillamine and N-acetylcysteine (NAcCys, with a blocked amino group)) as scavengers of methylglyoxal, compared with glutathione (GSH) and the biguanidine derivative metformin, was investigated. The time courses of the reactions of the aforementioned compounds with methylglyoxal were assayed. The reactivity of their thiol and amino groups decreased in the order of Cys > penicillamine > GSH > NAcCys and penicillamine > Cys > GSH, respectively. Human serum albumin (HSA) carbonylation in the absence or presence of methylglyoxal scavengers were monitored by the determination of the amino, guanidine and thiol groups' contents, as well as by spectrofluorimetry, CD and native and SDS PAGE. Cys and penicillamine were highly efficient in the prevention of the carbonylation of the HSA-amino (for 80%) and guanidine (for 84% and 55%, respectively) groups and the formation of fluorescent AGEs. GSH and metformin exhibited medium efficiency (reduction of amino group's carbonylation for 60% and guanidine for about 30%); the least efficient was NAcCys. The presence of Cys, penicillamine and NAcCys led to an almost complete protection of the HSA-thiol group's carbonylation, whereas metformin was inefficient. The efficiency in the prevention of protein cross-linking increased in the order of metformin, NAcCys < GSH < penicillamine < Cys. Thus, the substances with an α-amino-β-mercapto-ethane group as a pharmacophore exhibit great potential as an efficient methylglyoxal scavengers, and are thus promising compounds for medicinal chemistry. In addition, they protect the HSA-SH group and preserve its antioxidative potential, which is very important for the HSA's function in vivo.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Aćimović JM,Penezić AZ,Pavićević ID,Jovanović VB,Mandić LMdoi
10.1039/c4mb00217bsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
2166-75issue
8eissn
1742-206Xissn
1742-2051journal_volume
10pub_type
杂志文章abstract::Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for recombinant proteins. However, in vitro methods for Drosophila cell and organ c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00155a
更新日期:2014-10-01 00:00:00
abstract::Schistosomiasis is a parasitic infection that is endemic in many developing countries in the tropics and subtropics afflicting more than 207 million people primarily in rural areas. After malaria, it is the second most important parasitic infection in terms of socio-economic and public health. Investigation of the hos...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00262c
更新日期:2011-05-01 00:00:00
abstract::We employed high-temperature classical molecular dynamics (MD) simulations to investigate the unfolding process of the wild-type (WT) and F508del-NBD1 domains of CFTR protein, with and without second-site mutations. To rationalize the in vitro behavior of F508del-NBD1, namely its lower folding yield and higher aggrega...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00193a
更新日期:2016-08-16 00:00:00
abstract::The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing sub...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b916228c
更新日期:2010-01-01 00:00:00
abstract::The search for molecules with activity against Mycobacterium tuberculosis (Mtb) is employing many approaches in parallel including high throughput screening and computational methods. We have developed a database (CDD TB) to capture public and private Mtb data while enabling data mining and collaborations with other r...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b917766c
更新日期:2010-05-01 00:00:00
abstract::It is well known that oligomeric/aggregated amyloid β peptides are a key player in the pathogenesis of Alzheimer's disease and that different nanoparticles influence oligomerization/aggregation processes in experiments in vitro. Our previous results demonstrated antiaggregation effects of magnetite nanoparticles in th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c003498c
更新日期:2010-11-01 00:00:00
abstract::A novel class of pH-sensitive PEG lipids bearing acid-cleavable acetal linkages and short PEG chains have been synthesised and used in ternary vector formulations. The cleavage pH was influenced by structural components including the terminal PEG moiety and spacer length. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b719782a
更新日期:2008-06-01 00:00:00
abstract::In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00203j
更新日期:2016-05-24 00:00:00
abstract::MicroiRNAs are genome encoded small double stranded RNAs that regulate expression of homologous mRNAs. With approximately 2500 human miRNAs and each having hundreds of potential mRNA targets, miRNA based gene regulation is quite pervasive in both development and disease. While there are numerous studies investigating ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00415b
更新日期:2016-01-01 00:00:00
abstract::Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO˙, ˙O(2)(-) and ˙OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO˙, HPLC-MS aided PC12 cell systems were developed, and the conversions of 2-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05037k
更新日期:2011-05-01 00:00:00
abstract::The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70616h
更新日期:2014-05-01 00:00:00
abstract::Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00268k
更新日期:2015-09-01 00:00:00
abstract::Diabetic nephropathy (DN) is a serious metabolic disease, and comprehensive understanding of its complex mechanism will help in preventing the onset and progression of DN. To reveal the systemic metabolic changes associated with renal injury, we performed 1H NMR-based metabonomic and multivariate analyses to analyze s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70212j
更新日期:2013-11-01 00:00:00
abstract::Biochemical modifications to mRNA, especially N6-methyladenosine (m6A) and 5-methylcytosine (m5C), have been recently shown to be associated with crucial biological functions. Despite the intriguing advancements, little is known so far about the dynamic landscape of RNA methylome across different cell types and how th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00604f
更新日期:2015-01-01 00:00:00
abstract::Protein phosphorylation is associated with most cell signaling and developmental processes in eukaryotes. Despite the vast extent of the phosphoproteome within the cell, connecting specific kinases with relevant targets remains a significant experimental frontier. The challenge of linking kinases and their substrates ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b801724g
更新日期:2008-09-01 00:00:00
abstract::A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity t...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25516f
更新日期:2013-06-01 00:00:00
abstract::TGF-β1, 2 and 3 cytokines are involved in many cellular processes including cell proliferation, differentiation, migration and survival. Whereas TGF-β2 and 3 play important roles in embryonic development, TGF-β1 is mostly implicated in controlling immune responses after birth. The production of TGF-β1 is a tightly reg...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c7mb00251c
更新日期:2017-09-26 00:00:00
abstract::The present study describes for the first time, a metabolic profile reflecting the osteoporosis progression in 364 pre- and postmenopausal Chinese women using GC-MS. In order to accurately evaluate the dynamic changes of metabolites along with estrogen deficiency and osteoporosis progression, we divided these subjects...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00181e
更新日期:2016-06-21 00:00:00
abstract::Deciphering functional interactions between proteins is one of the great challenges in biology. Sequence-based homology-free encoding schemes have been increasingly applied to develop promising protein-protein interaction (PPI) predictors by means of statistical or machine learning methods. Here we analyze the relatio...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05427b
更新日期:2012-04-01 00:00:00
abstract::Chronic heart failure (CHF), as a progressive clinical syndrome, is characterized by failure of enough blood supply from the heart to meet the body's metabolic demands, and there is intense interest in identifying novel biomarkers that could make contributions to the diagnosis of CHF. Metabolomics, compared with curre...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70227h
更新日期:2013-11-01 00:00:00
abstract::Unstable angina (UA) is one of the most dangerous types of coronary heart disease and has high mortality and morbidity rates worldwide. However, the diagnostic accuracy for UA is unsatisfactory in clinical practice. In this study, we investigated the application of plasma metabolomics in discovering potential biomarke...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70216b
更新日期:2013-12-01 00:00:00
abstract::Mirror image phage display is a straightforward approach to identify new potentially therapeutically active D-enantiomeric peptides. Such D-peptides are more resistant to proteolytic degradation compared to L-peptides. In this review, several examples of mirror image phage display derived D-peptides with therapeutical...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b904138a
更新日期:2009-08-01 00:00:00
abstract::Osteosarcoma (OS) is a highly malignant bone tumour, affecting mainly children and young adults between 10 and 20 years of age. It represents the most frequent primitive malignant tumour of the skeletal system and is characterized by an extremely aggressive clinical course, with rapid development of lung metastases. I...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70328b
更新日期:2014-06-01 00:00:00
abstract::Mimicking receptor flexibility during receptor-ligand binding is a challenging task in computational drug design since it is associated with a large increase in the conformational search space. In the present study, we have devised an in silico design strategy incorporating receptor flexibility in virtual screening to...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00307a
更新日期:2014-10-01 00:00:00
abstract::Biological networks are effective tools for studying molecular interactions. Modular structure, in which genes or proteins may tend to be associated with functional modules or protein complexes, is a remarkable feature of biological networks. Mining modular structure from biological networks enables us to focus on a s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00617e
更新日期:2016-11-15 00:00:00
abstract::Insight into the molecular mechanism of complex diseases is an important topic in the current bio-medical research. However, different from the single-gene disorders, high heterogeneity of many of the complex diseases prevents scientists from the exact understanding of the etiology. In this study, we used Myelodysplas...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05018d
更新日期:2011-06-01 00:00:00
abstract::Over the last few years important new insights into the process of long-range gene regulation have been obtained. Gene regulatory elements are found to engage in direct physical interactions with distant target genes and with loci on other chromosomes to modulate transcription. An overview of recently discovered long-...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b803580f
更新日期:2008-11-01 00:00:00
abstract::Remineralization of organic matter in deep-sea sediments is important in oceanic biogeochemical cycles, and bacteria play a major role in this process. Shewanella violacea DSS12 is a psychrophilic and piezophilic gamma-proteobacterium that was isolated from the surface layer of deep sea sediment at a depth of 5110 m. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c000396d
更新日期:2010-07-01 00:00:00
abstract::In this review we bring together recent results from our group focused towards the development of biosensors from single conically-shaped artificial nanopores. The nanopores, used in the work presented here, were prepared using the track-etch process. The fabrication of track-etched conical nanopores has been optimize...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b708725j
更新日期:2007-10-01 00:00:00
abstract::The advent of large scale sequencing methods has enabled analyses of the protein-coding parts of cancer genomes to find the mutated genes that cause common human cancers. Unbiased mutation analyses of human tumors originating in the breast, colon, brain, and pancreas have revealed genomic landscapes composed of a few ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b903502h
更新日期:2009-09-01 00:00:00