Abstract:
:This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Jeong HS,Choi SM,Kim HW,Park JW,Park HN,Park SM,Jang SK,Rhee YM,Kim BHdoi
10.1039/c2mb25470ksubject
Has Abstractpub_date
2013-05-01 00:00:00pages
948-51issue
5eissn
1742-206Xissn
1742-2051journal_volume
9pub_type
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