Proteomic approaches to studying protein tyrosine phosphatases.

abstract：:Intrinsically disordered regions in proteins are known to evolve rapidly while maintaining their function. However, given their lack of structure and sequence conservation, the means through which they stay functional is not clear. Poor sequence conservation also hampers the classification of these regions into functi...

journal_title：Molecular bioSystems

authors： Moesa HA,Wakabayashi S,Nakai K,Patil A

更新日期：2012-10-30 00:00:00

abstract：BACKGROUND:Candida albicans, the most common fungal pathogen related to colonization and biofilm formation on the surfaces of indwelling medical devices, shows high resistance to the most commonly used antifungal drugs. In this study, an ion-pairing chromatography-porous graphitic carbon column coupled with a time-of-f...

journal_title：Molecular bioSystems

authors： Chen X,Wu H,Cao Y,Yao X,Zhao L,Wang T,Yang Y,Lv D,Chai Y,Cao Y,Zhu Z

更新日期：2014-01-01 00:00:00

abstract：:The adaptation of the cellular network to functional changes, timing and patterning of gene expression is regulated by binding of transcription factors to gene regulatory elements, which in turn depends on co-occurring histone modifications. These two layers influence each other, enabling a further level of regulatory...

journal_title：Molecular bioSystems

authors： Schueler M,Zhang Q,Schlesinger J,Tönjes M,Sperling SR

更新日期：2012-02-01 00:00:00

abstract：:RNA-binding proteins (RBPs) are key regulators of gene expression. Some long non-coding RNAs (lncRNAs) affect gene expression by interacting with RBPs. However, whether this influences the biological characteristics of lncRNAs in diseases still remains unknown. Here, we classify lncRNAs into two categories, using the ...

journal_title：Molecular bioSystems

authors： Zhang Q,Wei Y,Yan Z,Wu C,Chang Z,Zhu Y,Li K,Xu Y

更新日期：2017-06-01 00:00:00

abstract：:To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO(3)/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence o...

journal_title：Molecular bioSystems

authors： Zhao D,Zhuo RX,Cheng SX

更新日期：2012-03-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor participating in numerous redox reactions. It is also a substrate for regulatory modifications of proteins and nucleic acids via the addition of ADP-ribose moieties or removal of acyl groups by transfer to ADP-ribose. In this study, we use in-depth seque...

journal_title：Molecular bioSystems

authors： de Souza RF,Aravind L

更新日期：2012-06-01 00:00:00

abstract：:A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells. ...

journal_title：Molecular bioSystems

authors： Moreau L,Barthélémy P,Li Y,Luo D,Prata CA,Grinstaff MW

更新日期：2005-09-01 00:00:00

abstract：:The search for molecules with activity against Mycobacterium tuberculosis (Mtb) is employing many approaches in parallel including high throughput screening and computational methods. We have developed a database (CDD TB) to capture public and private Mtb data while enabling data mining and collaborations with other r...

journal_title：Molecular bioSystems

authors： Ekins S,Bradford J,Dole K,Spektor A,Gregory K,Blondeau D,Hohman M,Bunin BA

更新日期：2010-05-01 00:00:00

abstract：:Protein-protein interactions play an essential role in the regulation of various cellular processes. Understanding the recognition mechanism of protein-protein complexes is a challenging task in molecular and computational biology. In this work, we have developed an energy based approach for identifying the binding si...

journal_title：Molecular bioSystems

authors： Gromiha MM,Yokota K,Fukui K

更新日期：2009-12-01 00:00:00

abstract：:Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...

journal_title：Molecular bioSystems

authors： Kang A,Tan MH,Ling H,Chang MW

更新日期：2013-02-02 00:00:00

abstract：:Endogenous thymidylate synthases, isolated from tissues or cultured cells of the same specific origin, have been reported to show differing slow-binding inhibition patterns. These were reflected by biphasic or linear dependence of the inactivation rate on time and accompanied by differing inhibition parameters. Consid...

journal_title：Molecular bioSystems

authors： Ludwiczak J,Maj P,Wilk P,Frączyk T,Ruman T,Kierdaszuk B,Jarmuła A,Rode W

更新日期：2016-04-01 00:00:00

abstract：:The present study describes for the first time, a metabolic profile reflecting the osteoporosis progression in 364 pre- and postmenopausal Chinese women using GC-MS. In order to accurately evaluate the dynamic changes of metabolites along with estrogen deficiency and osteoporosis progression, we divided these subjects...

journal_title：Molecular bioSystems

authors： Qi H,Bao J,An G,Ouyang G,Zhang P,Wang C,Ying H,Ouyang P,Ma B,Zhang Q

更新日期：2016-06-21 00:00:00

abstract：:We demonstrate the expected preference of an immobilised oligosaccharide Man(9)(GlcNAc)(2) upon a 96-well photochemical array, for its known receptor, the cell-surface lectin Dendritic Cell-Specific ICAM3 Grabbing Nonintegrin (DC-SIGN) when compared to immobilised competing monosaccharides. ...

journal_title：Molecular bioSystems

authors： Dilly SJ,Clark AJ,Mitchell DA,Marsh A,Taylor PC

更新日期：2011-01-01 00:00:00

abstract：:Schistosomiasis is a parasitic infection that is endemic in many developing countries in the tropics and subtropics afflicting more than 207 million people primarily in rural areas. After malaria, it is the second most important parasitic infection in terms of socio-economic and public health. Investigation of the hos...

journal_title：Molecular bioSystems

authors： Balog CI,Meissner A,Göraler S,Bladergroen MR,Vennervald BJ,Mayboroda OA,Deelder AM

更新日期：2011-05-01 00:00:00

abstract：:New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...

journal_title：Molecular bioSystems

authors： Greco SJ,Rameshwar P

更新日期：2010-02-01 00:00:00

abstract：:Cell penetrating peptides (CPPs), TATp, in particular, has been used widely for intracellular delivery of various agents ranging from small molecules to proteins, peptides, range of pharmaceutical nanocarriers and imaging agents. This review highlights the mechanisms of CPP-mediated delivery and summarizes numerous ex...

journal_title：Molecular bioSystems

authors： Sawant R,Torchilin V

更新日期：2010-04-01 00:00:00

abstract：:To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...

journal_title：Molecular bioSystems

authors： Nikolov M,Fischle W

更新日期：2013-02-02 00:00:00

abstract：:Induced reprogramming of somatic cells has had a great impact on stem cell research, and the reprogramming technologies have evolved from four transgenic factors (Oct4, Sox2, Klf4, and c-Myc; OSKM) to just a few microRNAs (mainly miR-290/302 seed family). Despite these advances, the molecular events occurring during v...

journal_title：Molecular bioSystems

authors： Yang CS,Rana TM

更新日期：2013-01-27 00:00:00

abstract：:There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in ger...

journal_title：Molecular bioSystems

authors： Rejzek M,Stevenson CE,Southard AM,Stanley D,Denyer K,Smith AM,Naldrett MJ,Lawson DM,Field RA

更新日期：2011-03-01 00:00:00

abstract：:Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...

journal_title：Molecular bioSystems

authors： Tabor JJ,Bayer TS,Simpson ZB,Levy M,Ellington AD

更新日期：2008-07-01 00:00:00

abstract：:Cell culture metabolomics has demonstrated significant advantages in cancer research. However, its applications have been impeded by some influencing factors such as culture media, which could significantly affect cellular metabolic profiles and lead to inaccuracy and unreliability of comparative metabolomic analysis ...

journal_title：Molecular bioSystems

authors： Huang Z,Shao W,Gu J,Hu X,Shi Y,Xu W,Huang C,Lin D

更新日期：2015-07-01 00:00:00

abstract：:The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...

journal_title：Molecular bioSystems

authors： Li JJ,Cheng P,Tu J,Zhai HL,Zhang XY

更新日期：2016-02-01 00:00:00

abstract：:Ligand-induced conditional protein splicing (CPS) using a split intein allows the covalent reconstitution of a protein from two polypeptide fragments. The small molecule rapamycin binds to the fused FKBP and FRB dimerizer domains and thereby induces folding of the split intein, which then removes itself in the trans-s...

journal_title：Molecular bioSystems

authors： Sonntag T,Mootz HD

更新日期：2011-06-01 00:00:00

abstract：:Lovastatin and other statins inhibit HMG-CoA reductase, which carries out an early step in the sterol biosynthesis pathway. Statins lower cholesterol and are widely prescribed to prevent heart disease, but like many drugs, they can interact with nutritionally acquired metabolites. To probe these interactions, we explo...

journal_title：Molecular bioSystems

authors： Fowler DM,Cooper SJ,Stephany JJ,Hendon N,Nelson S,Fields S

更新日期：2011-02-01 00:00:00

abstract：:The induction of fungal metabolites by fungal co-cultures grown on solid media was explored using multi-well co-cultures in 2 cm diameter Petri dishes. Fungi were grown in 12-well plates to easily and rapidly obtain the large number of replicates necessary for employing metabolomic approaches. Fungal culture using suc...

journal_title：Molecular bioSystems

authors： Bertrand S,Azzollini A,Schumpp O,Bohni N,Schrenzel J,Monod M,Gindro K,Wolfender JL

更新日期：2014-07-29 00:00:00

abstract：:With the advent of new cancer therapies in the last few years, the goals of reducing disease burden and improving quality of life are frequently achieved. Yet despite the advances seen with numerous monotherapies, a multimodality approach that targets different aspects of tumor biology may yield the greatest clinical ...

journal_title：Molecular bioSystems

authors： Kamrava M,Bernstein MB,Camphausen K,Hodge JW

更新日期：2009-11-01 00:00:00

abstract：:Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...

journal_title：Molecular bioSystems

authors： Yan B,Deng Y,Hou J,Bi Q,Yang M,Jiang B,Liu X,Wu W,Guo D

更新日期：2015-02-01 00:00:00

abstract：:Fluorescent molecules that specifically target amyloid structures are highly desirable for amyloid research. Herein, we show a dimeric design of thioflavin T improves its binding affinity to Abeta amyloid by up to 70 fold, while not sacrificing the specificity and the "light-up" feature upon amyloid binding. ...

journal_title：Molecular bioSystems

authors： Qin L,Vastl J,Gao J

更新日期：2010-10-01 00:00:00

abstract：:Metabolomics is a rapidly growing branch of post-genomic chemical biology. The fission yeast Schizosaccharomyces pombe is an excellent eukaryotic model organism. Although the entire S. pombe genome has been sequenced and detailed transcriptomic analyses were performed, little metabolic profiling has been done. Here we...

journal_title：Molecular bioSystems

authors： Pluskal T,Nakamura T,Villar-Briones A,Yanagida M

更新日期：2010-01-01 00:00:00

abstract：:Homogeneous oligodeoxyguanylates incorporating small-molecule intercalator pyrene moieties in a 1,5 relationship form various structures of dimer, trimer, tetramer and internal hairpin form that are yellowish in color because of the intermolecular interactions between the pyrene units. ...

journal_title：Molecular bioSystems

authors： Jeong HS,Park M,Yi JW,Joo T,Kim BH

更新日期：2010-06-01 00:00:00