Ion-pairing chromatography on a porous graphitic carbon column coupled with time-of-flight mass spectrometry for targeted and untargeted profiling of amino acid biomarkers involved in Candida albicans biofilm formation.

abstract：:The heterogeneity of genetic alterations in human cancer genomes presents a major challenge to advancing our understanding of cancer mechanisms and identifying cancer driver genes. To tackle this heterogeneity problem, many approaches have been proposed to investigate genetic alterations and predict driver genes at th...

journal_title：Molecular bioSystems

authors： Gu Y,Wang H,Qin Y,Zhang Y,Zhao W,Qi L,Zhang Y,Wang C,Guo Z

更新日期：2013-03-01 00:00:00

abstract：:A novel class of pH-sensitive PEG lipids bearing acid-cleavable acetal linkages and short PEG chains have been synthesised and used in ternary vector formulations. The cleavage pH was influenced by structural components including the terminal PEG moiety and spacer length. ...

journal_title：Molecular bioSystems

authors： Wong JB,Grosse S,Tabor AB,Hart SL,Hailes HC

更新日期：2008-06-01 00:00:00

abstract：:Tankyrases (TNKS), key transmitters in the Wnt signaling pathway which is very conservative in evolution, are vital targets as they are overexpressed widely in many cancers. In this work, 5 inhibitors with novel structures have been discovered and validated using the ligand-based (pharmacophore) virtual screening, doc...

journal_title：Molecular bioSystems

authors： Pu Y,Zhang S,Chang Z,Zhang Y,Wang D,Zhang L,Li Y,Zuo Z

更新日期：2017-01-31 00:00:00

abstract：:Fabry disease (FD) is an X-linked lysosomal storage disorder caused by a deficiency of the lysosomal hydrolase α-galactosidase A (α-GalA) that leads to the intra-lysosomal accumulation of globotriaosylceramide (Gb3) in various organ systems. As a consequence, a multisystems disorder develops, culminating in stroke, pr...

journal_title：Molecular bioSystems

authors： Matafora V,Cuccurullo M,Beneduci A,Petrazzuolo O,Simeone A,Anastasio P,Mignani R,Feriozzi S,Pisani A,Comotti C,Bachi A,Capasso G

更新日期：2015-06-01 00:00:00

abstract：:Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initial step of the DNA injection process. Cell-puncturing protein needles are thoug...

journal_title：Molecular bioSystems

authors： Sanghamitra NJ,Inaba H,Arisaka F,Ohtan Wang D,Kanamaru S,Kitagawa S,Ueno T

更新日期：2014-10-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:Protein phosphorylation is associated with most cell signaling and developmental processes in eukaryotes. Despite the vast extent of the phosphoproteome within the cell, connecting specific kinases with relevant targets remains a significant experimental frontier. The challenge of linking kinases and their substrates ...

journal_title：Molecular bioSystems

authors： Sopko R,Andrews BJ

更新日期：2008-09-01 00:00:00

abstract：:Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...

journal_title：Molecular bioSystems

authors： Li S,Wang Y,Zhang J,Yang WH,Dai ZH,Zhu W,Yu XQ

更新日期：2011-04-01 00:00:00

abstract：:Upon rapid ascent to a high altitude, non-acclimatized individuals, although healthy, are highly prone to contracting high-altitude pulmonary edema (HAPE). Early diagnosis is difficult and there is no reliable biomarker available. We used proton ((1)H) NMR metabolomics to profile the altered metabolic patterns of bloo...

journal_title：Molecular bioSystems

authors： Luo Y,Zhu J,Gao Y

更新日期：2012-06-01 00:00:00

abstract：:In this review we bring together recent results from our group focused towards the development of biosensors from single conically-shaped artificial nanopores. The nanopores, used in the work presented here, were prepared using the track-etch process. The fabrication of track-etched conical nanopores has been optimize...

journal_title：Molecular bioSystems

authors： Sexton LT,Horne LP,Martin CR

更新日期：2007-10-01 00:00:00

abstract：:The neuroscientific community rapidly adopted RNA interference techniques as an experimental tool for the dissection of gene function in vitro and in animal models of neurological disease in vivo. Here, we discuss recent advances in the biotechnical implementation of siRNA/shRNA-mediated gene silencing focusing on iss...

journal_title：Molecular bioSystems

authors： Lingor P,Bähr M

更新日期：2007-11-01 00:00:00

abstract：:Regular physical activity is associated with a reduced risk of coronary heart disease, as it probably modifies the balance between free-radical generation and antioxidant activity. On the other hand, however, acute physical activity increases oxygen uptake and leads to a temporary imbalance between the production of r...

journal_title：Molecular bioSystems

authors： Guidi F,Magherini F,Gamberi T,Bini L,Puglia M,Marzocchini R,Ranaldi F,Modesti PA,Gulisano M,Modesti A

更新日期：2011-03-01 00:00:00

abstract：:In the field of synthetic biology, recent genetic engineering efforts have enabled the construction of novel genetic circuits with diverse functionalities and unique activation mechanisms. Because of these advances, artificial genetic networks are becoming increasingly complex, and are demonstrating more robust behavi...

journal_title：Molecular bioSystems

authors： Sayut DJ,Kambam PK,Sun L

更新日期：2007-12-01 00:00:00

abstract：:Calcium (Ca(2+)) is used as a signaling molecule to regulate many cellular processes. Calcium signaling generally involves transient elevations of the concentration of free Ca(2+) in the cytosol. More pronounced and sustained elevations of intracellular Ca(2+) concentrations are observed during apoptosis (programmed c...

journal_title：Molecular bioSystems

authors： Brnjic S,Olofsson MH,Havelka AM,Linder S

更新日期：2010-05-01 00:00:00

abstract：:Diabetic nephropathy (DN) is a serious metabolic disease, and comprehensive understanding of its complex mechanism will help in preventing the onset and progression of DN. To reveal the systemic metabolic changes associated with renal injury, we performed 1H NMR-based metabonomic and multivariate analyses to analyze s...

journal_title：Molecular bioSystems

authors： Liu J,Wang D,Chen Y,Sun H,He S,Wang C,Yang G,Shi M,Zhang J,Ren Y,Wang L,Lu Y,Cheng J

更新日期：2013-11-01 00:00:00

abstract：:Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather th...

journal_title：Molecular bioSystems

authors： Altman S

更新日期：2007-09-01 00:00:00

abstract：:The ectonucleotide phosphodiesterase/pyrophosphatase-1 (NPP1) is a type II transmembrane glycoprotein that regulates extracellular inorganic purine nucleotide and inorganic diphosphate levels through the hydrolysis of ATP into AMP and diphosphate. NPP1 is a promising drug target as it plays a role in several disorders...

journal_title：Molecular bioSystems

authors： Barbeau X,Mathieu P,Paquin JF,Lagüe P

更新日期：2017-05-30 00:00:00

abstract：:Cells of the bacterium Myxococcus xanthus organize into two types of patterns depending on their nutritional status, i.e. in the presence of nutrients cells form spreading colonies and in the absence of nutrients cells form fruiting bodies. Formation of both patterns depends on directed cell movements, which, in turn,...

journal_title：Molecular bioSystems

authors： Leonardy S,Bulyha I,Søgaard-Andersen L

更新日期：2008-10-01 00:00:00

abstract：:A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible bi...

journal_title：Molecular bioSystems

authors： Grognux J,Reymond JL

更新日期：2006-10-01 00:00:00

abstract：:Over the last few years important new insights into the process of long-range gene regulation have been obtained. Gene regulatory elements are found to engage in direct physical interactions with distant target genes and with loci on other chromosomes to modulate transcription. An overview of recently discovered long-...

journal_title：Molecular bioSystems

authors： Miele A,Dekker J

更新日期：2008-11-01 00:00:00

abstract：:Sequence specific binding of DNA and RNA is of fundamental importance in the regulation of cellular gene expression. Because of their modular structure repeat domain proteins are particularly well suited for these processes and have been widely adopted throughout evolution. Detailed biochemical and structural data has...

journal_title：Molecular bioSystems

authors： Filipovska A,Rackham O

更新日期：2012-03-01 00:00:00

abstract：:Recently, accumulating studies have indicated that microRNAs (miRNAs) play an important role in exploring the pathogenesis of various human diseases at the molecular level and may result in the design of specific tools for diagnosis, treatment evaluation and prevention. Experimental identification of disease-related m...

journal_title：Molecular bioSystems

authors： Sun D,Li A,Feng H,Wang M

更新日期：2016-06-21 00:00:00

abstract：:The small molecule 4-hydroxy-3-methoxybenzaldehyde (5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-4-yl)hydrazone (Exo2) stimulates morphological changes at the mammalian Golgi and trans-Golgi network that are virtually indistinguishable from those induced by brefeldin A. Both brefeldin A and Exo2 protect cells from...

journal_title：Molecular bioSystems

authors： Guetzoyan LJ,Spooner RA,Boal F,Stephens DJ,Lord JM,Roberts LM,Clarkson GJ

更新日期：2010-10-01 00:00:00

abstract：:The major mechanical function of talin is to couple the β-integrin cytoplasmic tails to actin filaments. A variety of β-integrin tails contain conserved binding motifs for talin, and recent research shows that β-integrins differ both in affinity to talin and preferences for other cytoplasmic adaptor proteins. While ta...

journal_title：Molecular bioSystems

authors： Kukkurainen S,Määttä JA,Saeger J,Valjakka J,Vogel V,Hytönen VP

更新日期：2014-12-01 00:00:00

abstract：:Enediyne natural product biosynthesis is characterized by a convergence of multiple pathways, generating unique peripheral moieties that are appended onto the distinctive enediyne core. Kedarcidin (KED) possesses two unique peripheral moieties, a (R)-2-aza-3-chloro-β-tyrosine and an iso-propoxy-bearing 2-naphthonate m...

journal_title：Molecular bioSystems

authors： Lohman JR,Huang SX,Horsman GP,Dilfer PE,Huang T,Chen Y,Wendt-Pienkowski E,Shen B

更新日期：2013-03-01 00:00:00

abstract：:The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual c...

journal_title：Molecular bioSystems

authors： Grande G,Milardi D,Vincenzoni F,Pompa G,Biscione A,Astorri AL,Fruscella E,De Luca A,Messana I,Castagnola M,Marana R

更新日期：2015-06-01 00:00:00

abstract：:We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...

journal_title：Molecular bioSystems

authors： Hill ZB,Perera BG,Maly DJ

更新日期：2011-02-01 00:00:00

abstract：:Unstable angina (UA) is one of the most dangerous types of coronary heart disease and has high mortality and morbidity rates worldwide. However, the diagnostic accuracy for UA is unsatisfactory in clinical practice. In this study, we investigated the application of plasma metabolomics in discovering potential biomarke...

journal_title：Molecular bioSystems

authors： Sun M,Gao X,Zhang D,Ke C,Hou Y,Fan L,Zhang R,Liu H,Li K,Yu B

更新日期：2013-12-01 00:00:00

abstract：:Cancers frequently develop resistance to paclitaxel but the underlying molecular mechanisms remain to be determined. We have investigated the proteins that are associated with the paclitaxel resistance in human breast cancer MCF-7 cells using proteomic analysis. Paclitaxel resistant human breast cancer MCF-7 cells (MC...

journal_title：Molecular bioSystems

authors： Chen S,Dong Q,Hu S,Cai J,Zhang W,Sun J,Wang T,Xie J,He H,Xing J,Lu J,Dong Y

更新日期：2014-02-01 00:00:00

abstract：:The induction of fungal metabolites by fungal co-cultures grown on solid media was explored using multi-well co-cultures in 2 cm diameter Petri dishes. Fungi were grown in 12-well plates to easily and rapidly obtain the large number of replicates necessary for employing metabolomic approaches. Fungal culture using suc...

journal_title：Molecular bioSystems

authors： Bertrand S,Azzollini A,Schumpp O,Bohni N,Schrenzel J,Monod M,Gindro K,Wolfender JL

更新日期：2014-07-29 00:00:00