Abstract:
:Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather than the "protein world".
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Altman Sdoi
10.1039/b707850csubject
Has Abstractpub_date
2007-09-01 00:00:00pages
604-7issue
9eissn
1742-206Xissn
1742-2051journal_volume
3pub_type
杂志文章,评审abstract::Transient and low-affinity interactions among macromolecules underlie many physiological events. Often, these interactions are difficult to study because they are not maintained when the participating molecules are removed from their cellular context. To circumvent this challenge, crosslinking reagents can be used to ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b803218a
更新日期:2008-06-01 00:00:00
abstract::Diabetic retinopathy (DR) is a serious microvascular syndrome of diabetes, and is one of the most frequent causes of blindness in the world. It has three progressive stages with complex metabolic deregulations in the holistic system of Western medicine. Chinese medicine classifies DR into two different syndrome types;...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00341g
更新日期:2011-07-01 00:00:00
abstract::The crystal structure of the versatile CYP109B1 enzyme from Bacillus subtilis has been solved at 1.8 Å resolution. This is the first structure of an enzyme from this CYP family, whose members are prevalent across diverse species of bacteria. In the crystal structure the enzyme has an open conformation with an access c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00665h
更新日期:2015-03-01 00:00:00
abstract::The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b815445g
更新日期:2009-02-01 00:00:00
abstract::The effect of the glutathione transferase P1-1 (GSTP1-1) targeting has been investigated in both sensitive (U-2OS) and cisplatin-resistant (U-2OS/CDDP4 μg) human osteosarcoma cell lines. Despite the different enzyme's content, inhibition of GSTP1-1 by 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX) causes the...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05295k
更新日期:2012-04-01 00:00:00
abstract::Escherichia coli has evolved an intracellular pathway to regulate its motion termed as chemotaxis so as to move towards a favorable environment such as regions with higher concentration of nutrients. Chemotaxis is a response to temporal and spatial variation of extracellular ligand concentration and randomness in moti...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b924368b
更新日期:2010-06-01 00:00:00
abstract::The investigation of the structure and dynamics of signal transduction systems through data-based mathematical models in ordinary differential equations or other paradigms has proven to be a successful approach in recent times. Extending this concept, we here analysed the use of kinetic models based on power-law terms...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00018c
更新日期:2010-11-01 00:00:00
abstract::A rapid and sensitive gold-nanobioprobe based immunoassay format has been presented for the detection of capsular Vi polysaccharide of Salmonella enterica serovar Typhi (surface antigen) using anti-Vi antibodies. The Vi antigen was extracted from serovar Typhi cells, under the optimised growth conditions for its over-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25048a
更新日期:2012-07-06 00:00:00
abstract::We employed high-temperature classical molecular dynamics (MD) simulations to investigate the unfolding process of the wild-type (WT) and F508del-NBD1 domains of CFTR protein, with and without second-site mutations. To rationalize the in vitro behavior of F508del-NBD1, namely its lower folding yield and higher aggrega...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00193a
更新日期:2016-08-16 00:00:00
abstract::Establishing functional relationships between multi-domain protein sequences is a non-trivial task. Traditionally, delineating functional assignment and relationships of proteins requires domain assignments as a prerequisite. This process is sensitive to alignment quality and domain definitions. In multi-domain protei...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70443b
更新日期:2014-05-01 00:00:00
abstract::Predicting protein subcellular localization is a challenging problem, particularly when query proteins have multi-label features meaning that they may simultaneously exist at, or move between, two or more different subcellular location sites. Most of the existing methods can only be used to deal with the single-label ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25466f
更新日期:2013-04-05 00:00:00
abstract::During the preclinical drug discovery process it remains a challenge to enable early elimination of candidate molecules that may have non-specific, off-target activities. Here, we use whole zebrafish embryo assays coupled with genetic analysis to address this issue. PLK1 (Polo-like kinase 1) is one of the key regulato...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b919743e
更新日期:2010-08-01 00:00:00
abstract::We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00108b
更新日期:2011-02-01 00:00:00
abstract::The induction of fungal metabolites by fungal co-cultures grown on solid media was explored using multi-well co-cultures in 2 cm diameter Petri dishes. Fungi were grown in 12-well plates to easily and rapidly obtain the large number of replicates necessary for employing metabolomic approaches. Fungal culture using suc...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00223g
更新日期:2014-07-29 00:00:00
abstract::Photo-chemical deamination of cytosine using 3-cyanovinylcarbazole nucleoside (CNVK) mediated photo-cross-linking is a technique for site-directed mutagenesis. Using this technique in vivo requires the elimination of a high-temperature incubation step; instead, incubation should be carried out under physiological cond...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00082k
更新日期:2017-06-01 00:00:00
abstract::The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique am...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b515777c
更新日期:2006-02-01 00:00:00
abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity t...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25516f
更新日期:2013-06-01 00:00:00
abstract::One of the fundamental goals in cellular biochemistry is to identify the functions of proteins in the context of compartments that organize them in the cellular environment. To realize this, it is indispensable to develop an automated method for fast and accurate identification of the subcellular locations of uncharac...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00267j
更新日期:2017-08-22 00:00:00
abstract::In vivo analysis is of paramount importance in monitoring physiological processes that take place in living organisms. Mass spectrometry, an analytical technique with high speed, sensitivity and specificity, is indispensable in biochemical studies nowadays. However, traditional mass spectrometric techniques are of lim...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25428j
更新日期:2013-05-01 00:00:00
abstract::Renal cell carcinoma (RCC), the most common neoplasm affecting the adult kidney, is characterised by heterogeneity of histological subtypes, drug resistance, and absence of molecular markers. Two-dimensional difference gel electrophoresis (2-D DIGE) technology in combination with mass spectrometry (MS) was applied to ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05390j
更新日期:2012-04-01 00:00:00
abstract::In the field of synthetic biology, recent genetic engineering efforts have enabled the construction of novel genetic circuits with diverse functionalities and unique activation mechanisms. Because of these advances, artificial genetic networks are becoming increasingly complex, and are demonstrating more robust behavi...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b700547d
更新日期:2007-12-01 00:00:00
abstract::Within systems biology there is an increasing interest in the stochastic behavior of genetic and biochemical reaction networks. An appropriate stochastic description is provided by the chemical master equation, which represents a continuous time Markov chain (CTMC). In this paper we consider the stochastic properties ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05086a
更新日期:2011-10-01 00:00:00
abstract::Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00214a
更新日期:2015-08-01 00:00:00
abstract::The identification of genetic markers (e.g. genes, pathways and subnetworks) for cancer has been one of the most challenging research areas in recent years. A subset of these studies attempt to analyze genome-wide expression profiles to identify markers with high reliability and reusability across independent whole-tr...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00684h
更新日期:2016-03-01 00:00:00
abstract::Upon entering biological systems, such as the blood stream, nanoparticles form molecular complexes with the proteins encountered called protein coronas, which shield the surface of the exogenous nanoparticle. The most abundant blood proteins, such as albumin, initially occupy the surface of the nanoparticle. Owing to ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00591k
更新日期:2015-02-01 00:00:00
abstract::MiRNAs are a fascinating kind of biomolecule due to their vital functions in gene regulation and potential value as biomarkers for serious diseases including cancers. Exploiting convenient and sensitive methods for miRNA expression assays is imperative. In this study, we employ an exonuclease (RecJf) and a nicking end...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00659k
更新日期:2016-11-15 00:00:00
abstract::Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b716221a
更新日期:2008-04-01 00:00:00
abstract::Cervical cancer is initiated by infection with high-risk human papillomavirus (HPV). The viral E6 and E7 oncogenes are required for the initiation of cervical epithelial cell immortalization, but do not suffice to cause cervical cancer. Human oncogenes and tumor suppressor genes with altered activity in cervical carci...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00284d
更新日期:2011-05-01 00:00:00
abstract::Calcium (Ca(2+)) is used as a signaling molecule to regulate many cellular processes. Calcium signaling generally involves transient elevations of the concentration of free Ca(2+) in the cytosol. More pronounced and sustained elevations of intracellular Ca(2+) concentrations are observed during apoptosis (programmed c...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b920805d
更新日期:2010-05-01 00:00:00