Abstract:
:MiRNAs are a fascinating kind of biomolecule due to their vital functions in gene regulation and potential value as biomarkers for serious diseases including cancers. Exploiting convenient and sensitive methods for miRNA expression assays is imperative. In this study, we employ an exonuclease (RecJf) and a nicking endonuclease (Nt.BbvCI) to catalyse isothermal reactions for the amplified detection of miRNA. The degree of cyclical enzymatic amplification depends on the initial target miRNA level, which can determine the density of DNA probes bound on the electrode surface. Since DNA probes with an amino group at the 3' end are able to locate silver nanoparticles on the electrode, which provide intense stripping responses, the sensitive quantification of miRNA can be achieved. The proposed method has a limit of detection as low as 35 aM, with remarkable specificity, which offers a new approach for investigating miRNA networks and for clinical diagnosis applications.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Xu J,Han K,Liu D,Lin L,Miao Pdoi
10.1039/c6mb00659ksubject
Has Abstractpub_date
2016-11-15 00:00:00pages
3550-3555issue
12eissn
1742-206Xissn
1742-2051journal_volume
12pub_type
杂志文章abstract::A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b503302k
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journal_title:Molecular bioSystems
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journal_title:Molecular bioSystems
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journal_title:Molecular bioSystems
pub_type: 杂志文章
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