Progress and challenges in predicting protein methylation sites.

abstract：:Disordered regions within proteins have increasingly been associated with various cellular functions. Identifying the specific roles played by disorder in these functions has proved difficult. However, the development of reliable prediction algorithms has expanded the study of disorder from a few anecdotal examples to...

journal_title：Molecular bioSystems

authors： Bellay J,Michaut M,Kim T,Han S,Colak R,Myers CL,Kim PM

更新日期：2012-01-01 00:00:00

abstract：:Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies d...

journal_title：Molecular bioSystems

authors： Ven Chang I,Tsutsumi H,Mihara H

更新日期：2017-10-24 00:00:00

abstract：:Over the past decade there has been a growing acknowledgement that a large proportion of proteins within most proteomes contain disordered regions. Disordered regions are segments of the protein chain which do not adopt a stable structure. Recognition of disordered regions in a protein is of great importance for prote...

journal_title：Molecular bioSystems

authors： Deng X,Eickholt J,Cheng J

更新日期：2012-01-01 00:00:00

abstract：:Unbalanced uptake of Omega 6/Omega 3 (ω-6/ω-3) ratios could increase chronic disease occurrences, such as inflammation, atherosclerosis, or tumor proliferation, and methylation methods for measuring the ruminal microbiome fatty acid (FA) composition/distribution play a vital role in discovering the contribution of foo...

journal_title：Molecular bioSystems

authors： Liu Y,Buendía-Rodríguez G,Peñuelas-Rívas CG,Tan Z,Rívas-Guevara M,Tenorio-Borroto E,Munteanu CR,Pazos A,González-Díaz H

更新日期：2015-11-01 00:00:00

abstract：:Cyclic adenosine monophosphate and cyclic guanosine monophosphate are universal intracellular messengers whose concentrations are regulated by molecular networks comprised of different isoforms of the synthases adenylate cyclase or guanylate cyclase and the phosphodiesterases which degrade these compounds. In this pap...

journal_title：Molecular bioSystems

authors： Valeyev NV,Heslop-Harrison P,Postlethwaite I,Gizatullina AN,Kotov NV,Bates DG

更新日期：2009-01-01 00:00:00

abstract：:Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, inc...

journal_title：Molecular bioSystems

authors： Alford SC,O'Sullivan C,Obst J,Christie J,Howard PL

更新日期：2014-04-01 00:00:00

abstract：:During the preclinical drug discovery process it remains a challenge to enable early elimination of candidate molecules that may have non-specific, off-target activities. Here, we use whole zebrafish embryo assays coupled with genetic analysis to address this issue. PLK1 (Polo-like kinase 1) is one of the key regulato...

journal_title：Molecular bioSystems

authors： Zhong H,Xin S,Zhao Y,Lu J,Li S,Gong J,Yang Z,Lin S

更新日期：2010-08-01 00:00:00

abstract：:Cervical cancer is a clinical and pathological heterogeneity disease, which requires different types of treatments and leads to a variety of outcomes. In clinical practice, only some patients benefit from chemotherapy treatment. Identifying patients who will be responsive to chemotherapy could increase their survival ...

journal_title：Molecular bioSystems

authors： Hou Y,Yin M,Sun F,Zhang T,Zhou X,Li H,Zheng J,Chen X,Li C,Ning X,Lou G,Li K

更新日期：2014-08-01 00:00:00

abstract：:Rhein is a natural product purified from herbal plants such as Rheum palmatum, which has been shown to have anti-angiogenesis and anti-tumor metastasis properties. However, the biological effects of rhein on the behavior of breast cancers are not completely elucidated. To evaluate whether rhein might be useful in the ...

journal_title：Molecular bioSystems

authors： Huang HJ,Lin CC,Chou HC,Chen YW,Lin ST,Lin YC,Lin DY,Lyu KW,Chan HL

更新日期：2014-12-01 00:00:00

abstract：:Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initial step of the DNA injection process. Cell-puncturing protein needles are thoug...

journal_title：Molecular bioSystems

authors： Sanghamitra NJ,Inaba H,Arisaka F,Ohtan Wang D,Kanamaru S,Kitagawa S,Ueno T

更新日期：2014-10-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:Upon entering biological systems, such as the blood stream, nanoparticles form molecular complexes with the proteins encountered called protein coronas, which shield the surface of the exogenous nanoparticle. The most abundant blood proteins, such as albumin, initially occupy the surface of the nanoparticle. Owing to ...

journal_title：Molecular bioSystems

authors： Ramezani F,Rafii-Tabar H

更新日期：2015-02-01 00:00:00

abstract：:A metabolomic method using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to obtain a systematic view of the development and progression of myocardial infarction (MI). By combining with partial least squares discriminant analysis (PLS-DA), 16 biomarkers in ...

journal_title：Molecular bioSystems

authors： Jiang P,Dai W,Yan S,Chen Z,Xu R,Ding J,Xiang L,Wang S,Liu R,Zhang W

更新日期：2011-03-01 00:00:00

abstract：:To identify an agent with specific activity against human lens epithelial cells (HLECs), we confirmed the presence of glucocorticoid receptors (GRs) and GR-α genes and evaluated whether GRs have a relationship with the apoptotic process in cultured HLECs. We also determined whether the inhibitor RU486 could rescue the...

journal_title：Molecular bioSystems

authors： Wang L,Zhao W,Leng F,Ge J,Bu Z,Zhang Y,Liu P

更新日期：2011-06-01 00:00:00

abstract：:Epithelial ovarian cancer (EOC) is the most lethal of gynecologic malignancies due to the high rate of recurrence and poor prognosis. Predicting the prognosis in patients with EOC is clinically challenging, partly because appropriate biomarkers of recurrence have yet to be explored. In this prospective study, pre-trea...

journal_title：Molecular bioSystems

authors： Zhang H,Ge T,Cui X,Hou Y,Ke C,Yang M,Yang K,Wang J,Guo B,Zhang F,Lou G,Li K

更新日期：2015-02-01 00:00:00

abstract：:Understanding the complexity of cancer biology requires extensive information about the cancer proteome over the course of the disease. The recent advances in mass spectrometry-based proteomics technologies have led to the accumulation of an incredible amount of such proteomic information. This information allows us t...

journal_title：Molecular bioSystems

authors： Miah S,Banks CA,Adams MK,Florens L,Lukong KE,Washburn MP

更新日期：2016-12-20 00:00:00

abstract：:The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...

journal_title：Molecular bioSystems

authors： Pirro V,Guffey SC,Sepúlveda MS,Mahapatra CT,Ferreira CR,Jarmusch AK,Cooks RG

更新日期：2016-06-01 00:00:00

abstract：:The recognition and association between the Ca2+/calmodulin-activated protein kinase II-α (CaMKIIα) and the multi-PDZ domain protein 1 (MUPP1) plays an important role in the sperm acrosome reaction and human fertilization. Previously, we have demonstrated that the MUPP1 PDZ11 domain is the primary binding partner of t...

journal_title：Molecular bioSystems

authors： Zhang YL,Han ZF

更新日期：2017-09-26 00:00:00

abstract：:One of the fundamental goals in cellular biochemistry is to identify the functions of proteins in the context of compartments that organize them in the cellular environment. To realize this, it is indispensable to develop an automated method for fast and accurate identification of the subcellular locations of uncharac...

journal_title：Molecular bioSystems

authors： Cheng X,Xiao X,Chou KC

更新日期：2017-08-22 00:00:00

abstract：:The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...

journal_title：Molecular bioSystems

authors： Kabir A,Kumar GS

更新日期：2014-05-01 00:00:00

abstract：:Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...

journal_title：Molecular bioSystems

authors： Lu B,Sun YX,Li YQ,Zhang XZ,Zhuo RX

更新日期：2009-06-01 00:00:00

abstract：:The biosynthesis of the endocannabinoid anandamide (AEA) and related N-acyl ethanolamine (NAE) lipids is complex and appears to involve multiple pathways, including: (1) direct release of NAEs from N-acyl phosphatidyl ethanolamine (NAPE) precursors by the phosphodiesterase NAPE-PLD, and (2) double O-deacylation of NAP...

journal_title：Molecular bioSystems

authors： Simon GM,Cravatt BF

更新日期：2010-08-01 00:00:00

abstract：:Drug combinations have been widely applied to treat complex diseases, like cancer, HIV and cardiovascular diseases. One of the most important characteristics for drug combinations is the synergistic effects among different drugs, that is to say, the combination effects are larger than the sum of individual effects. Al...

journal_title：Molecular bioSystems

authors： Chen D,Zhang H,Lu P,Liu X,Cao H

更新日期：2016-02-01 00:00:00

abstract：:There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in ger...

journal_title：Molecular bioSystems

authors： Rejzek M,Stevenson CE,Southard AM,Stanley D,Denyer K,Smith AM,Naldrett MJ,Lawson DM,Field RA

更新日期：2011-03-01 00:00:00

abstract：:All trans-retinoic acid (ATRA) is widely used to direct the differentiation of cultured stem cells. When exposed to the pluripotent human embryonal carcinoma (EC) stem cell line, TERA2.cl.SP12, ATRA induces ectoderm differentiation and the formation of neuronal cell types. We report in this study that novel polyene ch...

journal_title：Molecular bioSystems

authors： Clemens G,Flower KR,Gardner P,Henderson AP,Knowles JP,Marder TB,Whiting A,Przyborski S

更新日期：2013-12-01 00:00:00

abstract：:Histone deacetylases (HDACs) contribute to regulation of gene expression by mediating higher-order chromatin structures. They assemble into large multiprotein complexes that regulate activity and specificity. We report the development of small molecule probes with class IIa and pan-HDAC activity that contain photoreac...

journal_title：Molecular bioSystems

authors： Albrow VE,Grimley RL,Clulow J,Rose CR,Sun J,Warmus JS,Tate EW,Jones LH,Storer RI

更新日期：2016-05-24 00:00:00

abstract：:Glycodendrimers, such as glycoclusters and glycopolymers, are known to be very useful molecules to probe carbohydrate-lectin interactions. Herein, new second generation glycopeptide dendrimers (G2a-f) presenting a L-lysine-based (Lys) tetraantennary scaffold, four external thiomannosyl residues and, in the case of com...

journal_title：Molecular bioSystems

authors： Euzen R,Reymond JL

更新日期：2011-02-01 00:00:00

abstract：:Genome-wide RNA interference screens have greatly facilitated the identification of essential host factors (EHFs) for viral infections, whose knockdown effects significantly influence virus replication but not host cell viability. However, little has been done to link EHFs with another important host factor type, i.e....

journal_title：Molecular bioSystems

authors： Xie D,Han L,Luo Y,Liu Y,He S,Bai H,Wang S,Bo X

更新日期：2015-09-01 00:00:00

abstract：:Understanding the molecular mechanism that underlies the differentiation of neural stem cells (NSCs) is vital to develop regenerative medicines for neurological disorders. In our previous work, Rho-GDI-γ was found to be able to prompt neuronal differentiation when it was down regulated. However, it is unclear how Rho-...

journal_title：Molecular bioSystems

authors： Wang J,Hu F,Cheng H,Zhao XM,Wen T

更新日期：2012-11-01 00:00:00

abstract：:Endogenous thymidylate synthases, isolated from tissues or cultured cells of the same specific origin, have been reported to show differing slow-binding inhibition patterns. These were reflected by biphasic or linear dependence of the inactivation rate on time and accompanied by differing inhibition parameters. Consid...

journal_title：Molecular bioSystems

authors： Ludwiczak J,Maj P,Wilk P,Frączyk T,Ruman T,Kierdaszuk B,Jarmuła A,Rode W

更新日期：2016-04-01 00:00:00